Q2720799 (Q2720799): Difference between revisions
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(Created claim: summary (P836): The formation of fringing cells is a complex process that begins in the cytoplasm and is completed on the top surface of a cell. Fringes are involved in many functions important during fetal development and homeostasis of organisms. Anomalies in their creation and functioning are associated with a class of diseases, many of which occur in the Cypriot population. The identification of genes and mechanisms involved in the creation of fringes is es...) |
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The formation of fringing cells is a complex process that begins in the cytoplasm and is completed on the top surface of a cell. Fringes are involved in many functions important during fetal development and homeostasis of organisms. Anomalies in their creation and functioning are associated with a class of diseases, many of which occur in the Cypriot population. The identification of genes and mechanisms involved in the creation of fringes is essential for understanding the molecular background of these diseases and for the development of diagnostic and therapeutic methods. Previous work of our group led to the discovery of the clutch attachment complex. This complex consists of very well characterised proteins of the focal adhesions of cells. The CPCs are linked to the basic particles of fringes, in the absence of integrins, and interact with various actin networks. These networks are involved in processes of crucial importance for the proper creation and functioning of multicrossing cells, so understanding their interactions with CPCs is crucial. So far four proteins, components of CPCs, focal adhesion kinase (FAK), paxilin, talin and vinculin have been identified, but very little is known about their role in multicrossing cells. This proposal aims to identify all CPC proteins through proteomal analysis, and to determine the role of complexes in the creation and function of fringes. This will be achieved using advanced function loss techniques in combination with imaging techniques. At the same time, key questions will be answered concerning the mechanism by which CPCs are created and the function of various proteins in them. Finally, we propose the development of two innovative systems for the study of the molecular basis of diseases related to (English) | |||||||||||||||
| Property / summary: The formation of fringing cells is a complex process that begins in the cytoplasm and is completed on the top surface of a cell. Fringes are involved in many functions important during fetal development and homeostasis of organisms. Anomalies in their creation and functioning are associated with a class of diseases, many of which occur in the Cypriot population. The identification of genes and mechanisms involved in the creation of fringes is essential for understanding the molecular background of these diseases and for the development of diagnostic and therapeutic methods. Previous work of our group led to the discovery of the clutch attachment complex. This complex consists of very well characterised proteins of the focal adhesions of cells. The CPCs are linked to the basic particles of fringes, in the absence of integrins, and interact with various actin networks. These networks are involved in processes of crucial importance for the proper creation and functioning of multicrossing cells, so understanding their interactions with CPCs is crucial. So far four proteins, components of CPCs, focal adhesion kinase (FAK), paxilin, talin and vinculin have been identified, but very little is known about their role in multicrossing cells. This proposal aims to identify all CPC proteins through proteomal analysis, and to determine the role of complexes in the creation and function of fringes. This will be achieved using advanced function loss techniques in combination with imaging techniques. At the same time, key questions will be answered concerning the mechanism by which CPCs are created and the function of various proteins in them. Finally, we propose the development of two innovative systems for the study of the molecular basis of diseases related to (English) / rank | |||||||||||||||
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| Property / summary: The formation of fringing cells is a complex process that begins in the cytoplasm and is completed on the top surface of a cell. Fringes are involved in many functions important during fetal development and homeostasis of organisms. Anomalies in their creation and functioning are associated with a class of diseases, many of which occur in the Cypriot population. The identification of genes and mechanisms involved in the creation of fringes is essential for understanding the molecular background of these diseases and for the development of diagnostic and therapeutic methods. Previous work of our group led to the discovery of the clutch attachment complex. This complex consists of very well characterised proteins of the focal adhesions of cells. The CPCs are linked to the basic particles of fringes, in the absence of integrins, and interact with various actin networks. These networks are involved in processes of crucial importance for the proper creation and functioning of multicrossing cells, so understanding their interactions with CPCs is crucial. So far four proteins, components of CPCs, focal adhesion kinase (FAK), paxilin, talin and vinculin have been identified, but very little is known about their role in multicrossing cells. This proposal aims to identify all CPC proteins through proteomal analysis, and to determine the role of complexes in the creation and function of fringes. This will be achieved using advanced function loss techniques in combination with imaging techniques. At the same time, key questions will be answered concerning the mechanism by which CPCs are created and the function of various proteins in them. Finally, we propose the development of two innovative systems for the study of the molecular basis of diseases related to (English) / qualifier | |||||||||||||||
point in time: 31 May 2021
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Revision as of 19:26, 31 May 2021
Project Q2720799 in Cyprus
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | No label defined |
Project Q2720799 in Cyprus |
Statements
212,228.0 Euro
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249,680.0 Euro
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85.0 percent
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10 March 2017
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30 June 2022
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University of Cyprus
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Η δημιουργία των κροσσωτών κυττάρων είναι μια πολύπλοκη διαδικασία που ξεκινάει στο κυτταρόπλασμα και ολοκληρώνεται στην κορυφαία επιφάνεια ενός κυττάρου. Οι κροσσοί εμπλέκονται σε πολλές λειτουργίες σημαντικές κατά τη διάρκεια της εμβρυική ανάπτυξης και την ομοιόσταση των οργανισμών. Ανωμαλίες στη δημιουργία και λειτουργία τους συνδέονται με μια κατηγορία ασθενειών, πολλές από τις οποίες εμφανίζονται στον κυπριακό πληθυσμό. Η ταυτοποίηση γονιδίων και μηχανισμών που εμπλέκονται στη δημιουργία των κροσσών είναι απαραίτητη, για την κατανόηση του μοριακού υποβάθρου των ασθενειών αυτών και για την ανάπτυξη διαγνωστικών και θεραπευτικών μεθόδων. Προηγούμενη δουλεία της ομάδας μας οδήγησε στην ανακάλυψη του συμπλόκου προσκολλήσεως των κροσσών (ΣΠΚ). Αυτό το σύμπλοκο αποτελείται από πολύ καλά χαρακτηρισμένες πρωτεΐνες των εστιακών προσκολλήσεων των κυττάρων. Τα ΣΠΚ συνδέονται με τα βασικά σωμάτια των κροσσών, στην απουσία ιντεγκρινών, και αλληλεπιδρούν με διάφορα δίκτυα ακτίνης. Τα δίκτυα αυτά εμπλέκονται σε διαδικασίες καθοριστικής σημασίας για τη σωστή δημιουργία και λειτουργία των πολυκροσσωτών κυττάρων, γι’ αυτό η κατανόηση των αλληλεπιδράσεων τους με τα ΣΠΚ είναι καίριας σημασίας. Μέχρι στιγμής έχουν ταυτοποιηθεί τέσσερεις πρωτεΐνες, συστατικά των ΣΠΚ, η κινάση των εστιακών προσκολλήσεων (FAK), η παξιλίνη, η ταλίνη και η βινκουλίνη αλλά πολύ λίγα είναι γνωστά για τον ρόλο τους στα πολύκροσσωτά κύτταρα. Αυτή η πρόταση αποσκοπεί στην ταυτοποίηση του συνόλου των πρωτεϊνών των ΣΠΚ μέσω πρωτεωμικής ανάλυσης, και στoκαθορισμό του ρόλου των συμπλόκων στη δημιουργία και λειτουργία των κροσσωτών. Αυτό θα επιτευχθεί χρησιμοποιώντας προχωρημένες τεχνικές απώλειας λειτουργίας σε συνδυασμό με τεχνικές απεικόνισης. Ταυτόχρονα θα απαντηθούν βασικά ερωτήματα που αφορούν τον μηχανισμό με τον οποίο δημιουργούνται τα ΣΠΚ καθώς και τη λειτουργία διαφόρων πρωτεϊνών σε αυτά. Τέλος προτείνουμε την ανάπτυξη δύο καινοτόμων συστημάτων για τη μελέτη της μοριακής βάσης ασθενειών που σχετίζοντα (Greek)
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The formation of fringing cells is a complex process that begins in the cytoplasm and is completed on the top surface of a cell. Fringes are involved in many functions important during fetal development and homeostasis of organisms. Anomalies in their creation and functioning are associated with a class of diseases, many of which occur in the Cypriot population. The identification of genes and mechanisms involved in the creation of fringes is essential for understanding the molecular background of these diseases and for the development of diagnostic and therapeutic methods. Previous work of our group led to the discovery of the clutch attachment complex. This complex consists of very well characterised proteins of the focal adhesions of cells. The CPCs are linked to the basic particles of fringes, in the absence of integrins, and interact with various actin networks. These networks are involved in processes of crucial importance for the proper creation and functioning of multicrossing cells, so understanding their interactions with CPCs is crucial. So far four proteins, components of CPCs, focal adhesion kinase (FAK), paxilin, talin and vinculin have been identified, but very little is known about their role in multicrossing cells. This proposal aims to identify all CPC proteins through proteomal analysis, and to determine the role of complexes in the creation and function of fringes. This will be achieved using advanced function loss techniques in combination with imaging techniques. At the same time, key questions will be answered concerning the mechanism by which CPCs are created and the function of various proteins in them. Finally, we propose the development of two innovative systems for the study of the molecular basis of diseases related to (English)
31 May 2021
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*Δεν έχει γεωγραφική διάσταση*
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Identifiers
34491
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