FUNCTION OF MICRORNAS IN RESISTANCE TO PATHOGEN INFECTION IN PLANTS (Q3194714): Difference between revisions
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(Created claim: summary (P836): IN NATURE PLANTS ARE EXPOSED TO NUMEROUS ADVERSE ENVIRONMENTAL CONDITIONS INCLUDING BIOTIC AND ABIOTIC STRESS. TO ADAPT AND OVERCOME SUCH CONDITIONS, PLANTS DEVELOP DEFENSE MECHANISMS THAT ALLOW THEM TO RESPOND IN THE MOST APPROPRIATE WAY TO EACH TYPE OF STRESS. THIS REQUIRES SOPHISTICATED COMMUNICATION MECHANISMS BETWEEN SIGNALLING ROUTES INITIATED BY EXTERNAL FACTORS THAT ALLOW A COORDINATED RESPONSE IN THE PLANT. MICRORNAS (MIRNA) ARE KNOWN T...) |
(Changed label, description and/or aliases in en: translated_label) |
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FUNCTION OF MICRORNAS IN RESISTANCE TO PATHOGEN INFECTION IN PLANTS | |||
Revision as of 21:44, 12 October 2021
Project Q3194714 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | FUNCTION OF MICRORNAS IN RESISTANCE TO PATHOGEN INFECTION IN PLANTS |
Project Q3194714 in Spain |
Statements
163,350.0 Euro
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326,700.0 Euro
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50.0 percent
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1 January 2016
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30 June 2019
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CENTRO DE INVESTIGACION EN AGRIGENOMICA
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41°29'27.71"N, 2°8'15.00"E
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08266
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EN LA NATURALEZA LAS PLANTAS ESTAN EXPUESTAS A NUMEROSAS CONDICIONES AMBIENTALES ADVERSAS QUE INCLUYEN ESTRESES DE TIPO BIOTICO Y ABIOTICOS. PARA ADAPTARSE Y SUPERAR TALES CONDICIONES, LAS PLANTAS DESARROLLAN MECANISMOS DE DEFENSA QUE LES PERMITEN RESPONDER DE LA FORMA MAS APROPIADA A CADA TIPO DE ESTRES. ELLO REQUIERE SOFISTICADOS MECANISMOS DE COMUNICACION ENTRE VIAS DE SEÑALIZACION INICIADAS POR FACTORES EXTERNOS QUE PERMITAN UNA RESPUESTA COORDINADA EN LA PLANTA. SE SABE QUE LOS MICRORNA (MIRNA) SON REGULADORES DE LA EXPRESION GENICA EN DIFERENTES PROCESOS DEL DESARROLLO, ASI COMO EN RESPUESTAS ADAPTATIVAS A ESTRESES MEDIOAMBIENTALES. LOS MIRNAS PERMITEN DAR RESPUESTAS RAPIDA Y A VECES MUY ESPECIFICAS, POR LO QUE SON ELEMENTOS CLAVE PARA EL CORRECTO AJUSTE DE LA EXPRESSION GENICA EN PROCESOS DE RESISTENCIA A PATOGENOS, ASI COMO EN PROCESOS DE COMUNICACION EN LA RESPUESTA A DIFERENTES TIPOS DE ESTRES. ESTE PROYECTO SE ABORDA ESTE TEMA PARA DAR RESPUESTA A UNA PREGUNTA NUEVA SOBRE LA INTEGRACION DE PROCESOS DURANTE LA ADAPTACION A DIFERENTES ESTRESES AMBIENTALES: EL PAPEL DE LOS MIRNA EN LA RESPUESTA DE DEFENSA Y EN SU INTERACCION CON ESTRESES NUTRICIONALES EN PLANTAS. ESTOS ESTUDIOS SE LLEVARAN A CABO EN PLANTAS DE ARROZ Y ARABIDOPSIS, LAS DOS PLANTAS MODELO PARA ESTUDIOS DE GENOMICA FUNCIONAL EN MONOCOTILEDONEAS Y DICOTILEDONEAS, Y REPRESENTA LA CONTINUACION DEL PROYECTO ACTUAL (BIO2012-32838). CONCRETAMENTE,, SE PROPONE: I) CARACTERIZAR LA RELEVANCIA DE MIR399 Y MIR827 EN LA INTERACCION ENTRE LAS RUTAS DE SEÑALIZACION DE FOSFATO (P) Y LA RESPUESTA INMUNE (ARROZ, ARABIDOPSIS), Y II) AVERIGUAR EL PAPEL QUE TIENE MIR7695, UN MIRNA ESPECIFICO DE ARROZ IDENTIFICADO EN NUESTRO GRUPO, EN EL CONTROL DE LA HOMEOSTASIS DEL HIERRO (FE) Y/O MANGANESO (MN) DURANTE LA RESPUESTA DE DEFENSA (ARROZ). ASIMISMO, SE ESTUDIARA LA INTERACCION ENTRE RUTAS DE SEÑALIZACION DE P Y FE/MN IMPLICADAS EN EL FENOTIPO DE RESISTENCIA/SUSCEPTIBILIDAD DE LAS PLANTAS EN RESPUESTA A LA INFECCION. EN NUESTRO GRUPO SE ESTUDIAN LOS PATOSISTEMAS ADECUADOS PARA ESTOS ESTUDIOS: ARROZ/MAGNAPORTHE ORYZAE Y ARABIDOPSIS /PLECTOSPHAERELLA CUCUMERINA. POR OTRO LADO, EN LA LITERATURA SE HA DESCRITO QUE CIERTOS MIRNAS Y SIRNAS SON CAPACES DE SER TRANSPORTADOS Y ACTUAR EN ORGANISMOS DIFERENTES DEL QUE PROVIENEN (¿TRANS-KINGDOM CROSS-TALK¿). EN ESTE PROYECTO ANALIZAREMOS SI EXISTE UNA REGULACION MEDIADA POR MIRNAS ENTRE ESPECIES EN LOS PROCESOS DE RESISTENCIA A PATOGENOS EN ARROZ. ESTE PROYECTO ABARCA TECNICAS DE GENETICA DIRECTA Y REVERSA, SECUENCIACION MASIVA Y ANALISIS BIOINFORMATICO, ANALISIS CITOLOGICOS E HISTOLOGICOS, E IDENTIFICACION DE COMPLEJOS DE MIRNA-AGO DURANTE LOS PROCESOS DE INTERACCION PLANTA-PATOGENO. LA DISPONIBILIDAD DE PROTOCOLOS AND HERRAMIENTAS DE TRABAJO, NOS COLOCAN EN UN BUEN PUNTO DE PARTIDA PARA ALCANZAR DICHOS OBJETIVOS. EN CASO DE OBTENER RESULTADOS POSITIVOS, SE DEMOSTRARIA QUE LOS MIRNAS ACTUAN COMO ELEMENTOS DE CONEXION REGULADORES DE LA EXPRESION GENICA PARA EL CONTROL DE RESPUESTAS INMUNES Y HOMEOSTASIS DE NUTRIENTES DURANTE LA INFECCION POR PATOGENOS. LOS RESULTADOS OBTENIDOS Y EL CONOCIMIENTO GENERADO EN ESTE PROYECTO CONTRIBUIRAN AL ESTABLECIMIENTO DE NUEVOS CONCEPTOS EN ESTUDIOS DE INTERACCION PLANTA/PATOGENO. ELLO AYUDARA A DEFINIR NUEVAS ESTRATEGIAS DE MEJORA DE LA RESISTENCIA DE LAS PLANTAS A LA INFECCION POR PATOGENOS, QUE EN ULTIMO TERMINO BENEFICIARA A LOS PROGRAMAS DE MEJORA EN ESPECIES DE INTERES AGRONOMICO. (Spanish)
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IN NATURE PLANTS ARE EXPOSED TO NUMEROUS ADVERSE ENVIRONMENTAL CONDITIONS INCLUDING BIOTIC AND ABIOTIC STRESS. TO ADAPT AND OVERCOME SUCH CONDITIONS, PLANTS DEVELOP DEFENSE MECHANISMS THAT ALLOW THEM TO RESPOND IN THE MOST APPROPRIATE WAY TO EACH TYPE OF STRESS. THIS REQUIRES SOPHISTICATED COMMUNICATION MECHANISMS BETWEEN SIGNALLING ROUTES INITIATED BY EXTERNAL FACTORS THAT ALLOW A COORDINATED RESPONSE IN THE PLANT. MICRORNAS (MIRNA) ARE KNOWN TO BE REGULATORS OF GENIC EXPRESSION IN DIFFERENT DEVELOPMENTAL PROCESSES, AS WELL AS IN ADAPTIVE RESPONSES TO ENVIRONMENTAL STRESS. MIRNAS ALLOW RAPID AND SOMETIMES VERY SPECIFIC RESPONSES, SO THEY ARE KEY ELEMENTS FOR THE CORRECT ADJUSTMENT OF THE GENIC EXPRESSION IN PROCESSES OF RESISTANCE TO PATHOGENS, AS WELL AS IN COMMUNICATION PROCESSES IN THE RESPONSE TO DIFFERENT TYPES OF STRESS. THIS PROJECT ADDRESSES THIS TOPIC TO ANSWER A NEW QUESTION ABOUT THE INTEGRATION OF PROCESSES DURING THE ADAPTATION TO DIFFERENT ENVIRONMENTAL STRESS: THE ROLE OF THE MIRNA IN THE DEFENSE RESPONSE AND IN THEIR INTERACTION WITH NUTRITIONAL STRESS IN PLANTS. THESE STUDIES WILL BE CARRIED OUT IN RICE PLANTS AND ARABIDOPSIS, THE TWO MODEL PLANTS FOR STUDIES OF FUNCTIONAL GENOMIC IN MONOCOTYLEDONES AND DICOTYLEDONES, AND REPRESENTS THE CONTINUATION OF THE CURRENT PROJECT (BIO2012-32838). IN PARTICULAR, IT IS PROPOSED THAT: I) CHARACTERISE THE RELEVANCE OF MIR399 AND MIR827 IN THE INTERACTION BETWEEN THE PHOSPHATE SIGNALLING ROUTES (P) AND THE IMMUNE RESPONSE (RICE, ARABIDOPSIS), AND II) TO FIND OUT THE ROLE OF MIR7695, A SPECIFIC MIRNA OF RICE IDENTIFIED IN OUR GROUP, IN THE CONTROL OF THE HOMEOSTASIS OF IRON (FE) AND/OR MANGANESE (MN) DURING THE DEFENSE RESPONSE (RICE). IN ADDITION, THE INTERACTION BETWEEN SIGNALLING ROUTES OF P AND FAITH/MN INVOLVED IN THE PHENOTYPE OF RESISTANCE/SUSCEPTIBILITY OF PLANTS IN RESPONSE TO INFECTION WILL BE STUDIED. IN OUR GROUP THE APPROPRIATE PATHOSYSTEMS FOR THESE STUDIES ARE STUDIED: RICE/MAGNAPORTHE ORYZAE AND ARABIDOPSIS/PLECTOSPHAERELLA CUCUMERINA. ON THE OTHER HAND, IN THE LITERATURE IT HAS BEEN DESCRIBED THAT CERTAIN MIRNAS AND SIRNAS ARE CAPABLE OF BEING TRANSPORTED AND ACTING IN ORGANISMS OTHER THAN THE ONE THEY COME FROM (TRANS-KINGDOM CROSS-TALK). IN THIS PROJECT WE WILL ANALYSE WHETHER THERE IS A REGULATION MEDIATED BY MIRNAS BETWEEN SPECIES IN THE PROCESSES OF RESISTANCE TO PATHOGENS IN RICE. THIS PROJECT COVERS TECHNIQUES OF DIRECT AND REVERSE GENETIC, MASS SEQUENCING AND BIOINFORMATIC ANALYSIS, CYTOLOGIC AND HISTOLOGIC ANALYSIS, AND IDENTIFICATION OF MIRNA-AGO COMPLEXES DURING PLANT-PATHOGEN INTERACTION PROCESSES. THE AVAILABILITY OF PROTOCOLS AND WORK TOOLS, PUTS US IN A GOOD STARTING POINT TO ACHIEVE THESE GOALS. IF POSITIVE RESULTS ARE OBTAINED, IT WOULD BE DEMONSTRATED THAT MIRNAS ACT AS CONNECTING ELEMENTS REGULATING GENIC EXPRESSION FOR THE CONTROL OF IMMUNE RESPONSES AND NUTRIENT HOMEOSTASIS DURING PATHOGEN INFECTION. THE RESULTS OBTAINED AND THE KNOWLEDGE GENERATED IN THIS PROJECT WILL CONTRIBUTE TO THE ESTABLISHMENT OF NEW CONCEPTS IN PLANT/PATHOGEN INTERACTION STUDIES. THIS WILL HELP DEFINE NEW STRATEGIES TO IMPROVE THE RESISTANCE OF PLANTS TO PATHOGEN INFECTION, WHICH WILL ULTIMATELY BENEFIT IMPROVEMENT PROGRAMS IN SPECIES OF AGRONOMIC INTEREST. (English)
12 October 2021
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Cerdanyola del Vallès
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Identifiers
BIO2015-67212-R
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