APPLICATION OF ELECTROSPRAY MASS IONISATION SPECTROMETRY FOR RAPID IDENTIFICATION AND QUANTIFICATION OF FOOD PATHOGENS (Q3151710): Difference between revisions

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(‎Removed claim: summary (P836): THE INCREASE IN FOOD-BORNE DISEASES OVER THE LAST FEW YEARS, WITH A SPECIAL EMERGENCE OF PATHOGENIC MICROORGANISMS SUCH AS LISTERIA MONOCYTOGENES, SALMONELLA SPP, CAMPYLOBACTER JEJUNI, OR ESCHERICHIA COLI, HAS INCREASED CONCERNS ABOUT FOOD SAFETY BOTH AMONG AUTHORITIES AND IN THE PRODUCTIVE SECTOR AND AMONG CONSUMERS. FOOD PATHOGENS CANNOT BE COMPLETELY ELIMINATED FROM ALL STAGES OF THE FOOD CHAIN. IN ADDITION, ACCORDING TO THE TREATMENT SUFFE...)
Property / summary
THE INCREASE IN FOOD-BORNE DISEASES OVER THE LAST FEW YEARS, WITH A SPECIAL EMERGENCE OF PATHOGENIC MICROORGANISMS SUCH AS LISTERIA MONOCYTOGENES, SALMONELLA SPP, CAMPYLOBACTER JEJUNI, OR ESCHERICHIA COLI, HAS INCREASED CONCERNS ABOUT FOOD SAFETY BOTH AMONG AUTHORITIES AND IN THE PRODUCTIVE SECTOR AND AMONG CONSUMERS. FOOD PATHOGENS CANNOT BE COMPLETELY ELIMINATED FROM ALL STAGES OF THE FOOD CHAIN. IN ADDITION, ACCORDING TO THE TREATMENT SUFFERED BY THE FOOD, THE MICROBIOTA PRESENT MAY VARY. THERE ARE NUMEROUS PATHOGENS THAT THE PROCESSING COMPANY MAY BE REQUIRED TO ANALYSE TO DETERMINE THE RELATIVE SAFETY OF YOUR PRODUCT. THE DETECTION AND RAPID IDENTIFICATION METHODS CURRENTLY IMPLEMENTED PRESENTLY HAVE THE DISADVANTAGE OF BEING LIMITED TO A FEW SPECIES (REAL-TIME PCR), NEEDING LONG PROCESSING (SEQUENCE, DO), NOT BE QUANTITATIVE (SEQUENCING, MALDI-TOF), DO NOT ALLOW DETECTION IN COMPLEX MIXTURES SUCH AS FOOD (SEQUENCING, MALDI-TOF), AND PRACTICALLY ALL OF THEM NEED PREVIOUSLY PLATE SOWING. THAT IS WHY IT IS NECESSARY TO IMPLEMENT METHODS THAT ALLOW THE RAPID AND ACCURATE DETECTION OF CONTAMINATING SPECIES IN THE FOOD AS WELL AS THE QUANTIFICATION DIRECTLY IN A FOOD SAMPLE. THIS IS THE CASE WITH THE METHODOLOGY PROPOSED IN THIS PROJECT: THE DEFINITION OF BIOMARKING PEPTIDES OF PATHOGENOUS AND BIOGENOUS AMINE-PRODUCING SPECIES IN FOOD AND THE SUBSEQUENT IMPLEMENTATION OF THE APPLICATION OF THESE PEPTIDES FOR DETECTION IN FOOD SAMPLES. The LIQUID CROMATOGRAPHY ACOPLATE TO SPECTROMETRIA OF MASAS IN TANDEM IN an electrospray-TRAMPA IONICA EQUIPMENT (LC-ESI-IT-MS/MS) and POSTERIOR ANALISIS MEDIENT DIFFERENT SOFTWARE AND COMPARATION WITH AMINOACIDIC SECUTIONS deposited in DATA BASES._x000D_ in order to lead to the deposition of bio-marker PEPTIDs, it is necessary to provide for a full range of BACTERIAAN CEPAS to take over the Analyses. THE BACTERIAL SPECIES INCLUDED IN THE STUDY WILL INCLUDE THOSE THAT ARE MOST FREQUENTLY ISOLATED IN FOOD PRODUCTS, AS DESCRIBED IN THE LITERATURE, AND INCLUDE THE FAMILY ENTEROBACTERIACEAE, SALMONELLA, SHIGELLA, PROTEUS, COAGULASE-POSITIVE STAPHYLOCOCCI; CLOSTRIDIUM PERFRINGENS, L. MONOCYTOGENES, BACILLUS CEREUS, AND C. BOTULINUM TYPES E AND B, BACILLUS CEREUS, B. SUBTILIS AND B. LICHENIFORMIS, VIBRIO PARAHAEMOLYTICUS, CAMPYLOBACTER SPP., CAMPYLOBACTER JEJUNI, LISTERIA MONOCYTOGENES AND YERSINIA ENTEROCOLITICA, STAPHYLOCOCCUS AUREUS, BACILLUS SPP AND PAENIBACILLUS SPP. OUR LABORATORY HAS A WIDE COLLECTION OF BACTERIAL STRAINS ISOLATED FROM VARIOUS FOODS (MEAT, FISH, MINIMALLY PROCESSED AND READY FOR CONSUMPTION, MILK, ETC.). ALL OF THEM HAVE BEEN CHARACTERISED BY DIFFERENT METHODS, BOTH CLASSICAL AND GENOMIC AND PROTEOMIC (MALDI-TOF). Such RELATED SPECIES WITH YOUR SPECIFIC MALDI-TOF SPECTIVES AND GENOMIC SECUCE OF GEN 16 are deposited at the SPECTRABANK DATA BASE (WWW.SPECTRABANK.ORG) as well as in the GENBANK DATA BASE (NCBI) (only the SECUENCE OF GEN 16S rRNA)._x000D_ METODOLOGY AND RESULTS they will allow them to expand the SPECTRABANK DATA BASE (WWW.SPECTRABANK.ORG), developed in our laboratorium and constituting the highest international data base of access (English)
 
Property / summary: THE INCREASE IN FOOD-BORNE DISEASES OVER THE LAST FEW YEARS, WITH A SPECIAL EMERGENCE OF PATHOGENIC MICROORGANISMS SUCH AS LISTERIA MONOCYTOGENES, SALMONELLA SPP, CAMPYLOBACTER JEJUNI, OR ESCHERICHIA COLI, HAS INCREASED CONCERNS ABOUT FOOD SAFETY BOTH AMONG AUTHORITIES AND IN THE PRODUCTIVE SECTOR AND AMONG CONSUMERS. FOOD PATHOGENS CANNOT BE COMPLETELY ELIMINATED FROM ALL STAGES OF THE FOOD CHAIN. IN ADDITION, ACCORDING TO THE TREATMENT SUFFERED BY THE FOOD, THE MICROBIOTA PRESENT MAY VARY. THERE ARE NUMEROUS PATHOGENS THAT THE PROCESSING COMPANY MAY BE REQUIRED TO ANALYSE TO DETERMINE THE RELATIVE SAFETY OF YOUR PRODUCT. THE DETECTION AND RAPID IDENTIFICATION METHODS CURRENTLY IMPLEMENTED PRESENTLY HAVE THE DISADVANTAGE OF BEING LIMITED TO A FEW SPECIES (REAL-TIME PCR), NEEDING LONG PROCESSING (SEQUENCE, DO), NOT BE QUANTITATIVE (SEQUENCING, MALDI-TOF), DO NOT ALLOW DETECTION IN COMPLEX MIXTURES SUCH AS FOOD (SEQUENCING, MALDI-TOF), AND PRACTICALLY ALL OF THEM NEED PREVIOUSLY PLATE SOWING. THAT IS WHY IT IS NECESSARY TO IMPLEMENT METHODS THAT ALLOW THE RAPID AND ACCURATE DETECTION OF CONTAMINATING SPECIES IN THE FOOD AS WELL AS THE QUANTIFICATION DIRECTLY IN A FOOD SAMPLE. THIS IS THE CASE WITH THE METHODOLOGY PROPOSED IN THIS PROJECT: THE DEFINITION OF BIOMARKING PEPTIDES OF PATHOGENOUS AND BIOGENOUS AMINE-PRODUCING SPECIES IN FOOD AND THE SUBSEQUENT IMPLEMENTATION OF THE APPLICATION OF THESE PEPTIDES FOR DETECTION IN FOOD SAMPLES. The LIQUID CROMATOGRAPHY ACOPLATE TO SPECTROMETRIA OF MASAS IN TANDEM IN an electrospray-TRAMPA IONICA EQUIPMENT (LC-ESI-IT-MS/MS) and POSTERIOR ANALISIS MEDIENT DIFFERENT SOFTWARE AND COMPARATION WITH AMINOACIDIC SECUTIONS deposited in DATA BASES._x000D_ in order to lead to the deposition of bio-marker PEPTIDs, it is necessary to provide for a full range of BACTERIAAN CEPAS to take over the Analyses. THE BACTERIAL SPECIES INCLUDED IN THE STUDY WILL INCLUDE THOSE THAT ARE MOST FREQUENTLY ISOLATED IN FOOD PRODUCTS, AS DESCRIBED IN THE LITERATURE, AND INCLUDE THE FAMILY ENTEROBACTERIACEAE, SALMONELLA, SHIGELLA, PROTEUS, COAGULASE-POSITIVE STAPHYLOCOCCI; CLOSTRIDIUM PERFRINGENS, L. MONOCYTOGENES, BACILLUS CEREUS, AND C. BOTULINUM TYPES E AND B, BACILLUS CEREUS, B. SUBTILIS AND B. LICHENIFORMIS, VIBRIO PARAHAEMOLYTICUS, CAMPYLOBACTER SPP., CAMPYLOBACTER JEJUNI, LISTERIA MONOCYTOGENES AND YERSINIA ENTEROCOLITICA, STAPHYLOCOCCUS AUREUS, BACILLUS SPP AND PAENIBACILLUS SPP. OUR LABORATORY HAS A WIDE COLLECTION OF BACTERIAL STRAINS ISOLATED FROM VARIOUS FOODS (MEAT, FISH, MINIMALLY PROCESSED AND READY FOR CONSUMPTION, MILK, ETC.). ALL OF THEM HAVE BEEN CHARACTERISED BY DIFFERENT METHODS, BOTH CLASSICAL AND GENOMIC AND PROTEOMIC (MALDI-TOF). Such RELATED SPECIES WITH YOUR SPECIFIC MALDI-TOF SPECTIVES AND GENOMIC SECUCE OF GEN 16 are deposited at the SPECTRABANK DATA BASE (WWW.SPECTRABANK.ORG) as well as in the GENBANK DATA BASE (NCBI) (only the SECUENCE OF GEN 16S rRNA)._x000D_ METODOLOGY AND RESULTS they will allow them to expand the SPECTRABANK DATA BASE (WWW.SPECTRABANK.ORG), developed in our laboratorium and constituting the highest international data base of access (English) / rank
Normal rank
 
Property / summary: THE INCREASE IN FOOD-BORNE DISEASES OVER THE LAST FEW YEARS, WITH A SPECIAL EMERGENCE OF PATHOGENIC MICROORGANISMS SUCH AS LISTERIA MONOCYTOGENES, SALMONELLA SPP, CAMPYLOBACTER JEJUNI, OR ESCHERICHIA COLI, HAS INCREASED CONCERNS ABOUT FOOD SAFETY BOTH AMONG AUTHORITIES AND IN THE PRODUCTIVE SECTOR AND AMONG CONSUMERS. FOOD PATHOGENS CANNOT BE COMPLETELY ELIMINATED FROM ALL STAGES OF THE FOOD CHAIN. IN ADDITION, ACCORDING TO THE TREATMENT SUFFERED BY THE FOOD, THE MICROBIOTA PRESENT MAY VARY. THERE ARE NUMEROUS PATHOGENS THAT THE PROCESSING COMPANY MAY BE REQUIRED TO ANALYSE TO DETERMINE THE RELATIVE SAFETY OF YOUR PRODUCT. THE DETECTION AND RAPID IDENTIFICATION METHODS CURRENTLY IMPLEMENTED PRESENTLY HAVE THE DISADVANTAGE OF BEING LIMITED TO A FEW SPECIES (REAL-TIME PCR), NEEDING LONG PROCESSING (SEQUENCE, DO), NOT BE QUANTITATIVE (SEQUENCING, MALDI-TOF), DO NOT ALLOW DETECTION IN COMPLEX MIXTURES SUCH AS FOOD (SEQUENCING, MALDI-TOF), AND PRACTICALLY ALL OF THEM NEED PREVIOUSLY PLATE SOWING. THAT IS WHY IT IS NECESSARY TO IMPLEMENT METHODS THAT ALLOW THE RAPID AND ACCURATE DETECTION OF CONTAMINATING SPECIES IN THE FOOD AS WELL AS THE QUANTIFICATION DIRECTLY IN A FOOD SAMPLE. THIS IS THE CASE WITH THE METHODOLOGY PROPOSED IN THIS PROJECT: THE DEFINITION OF BIOMARKING PEPTIDES OF PATHOGENOUS AND BIOGENOUS AMINE-PRODUCING SPECIES IN FOOD AND THE SUBSEQUENT IMPLEMENTATION OF THE APPLICATION OF THESE PEPTIDES FOR DETECTION IN FOOD SAMPLES. The LIQUID CROMATOGRAPHY ACOPLATE TO SPECTROMETRIA OF MASAS IN TANDEM IN an electrospray-TRAMPA IONICA EQUIPMENT (LC-ESI-IT-MS/MS) and POSTERIOR ANALISIS MEDIENT DIFFERENT SOFTWARE AND COMPARATION WITH AMINOACIDIC SECUTIONS deposited in DATA BASES._x000D_ in order to lead to the deposition of bio-marker PEPTIDs, it is necessary to provide for a full range of BACTERIAAN CEPAS to take over the Analyses. THE BACTERIAL SPECIES INCLUDED IN THE STUDY WILL INCLUDE THOSE THAT ARE MOST FREQUENTLY ISOLATED IN FOOD PRODUCTS, AS DESCRIBED IN THE LITERATURE, AND INCLUDE THE FAMILY ENTEROBACTERIACEAE, SALMONELLA, SHIGELLA, PROTEUS, COAGULASE-POSITIVE STAPHYLOCOCCI; CLOSTRIDIUM PERFRINGENS, L. MONOCYTOGENES, BACILLUS CEREUS, AND C. BOTULINUM TYPES E AND B, BACILLUS CEREUS, B. SUBTILIS AND B. LICHENIFORMIS, VIBRIO PARAHAEMOLYTICUS, CAMPYLOBACTER SPP., CAMPYLOBACTER JEJUNI, LISTERIA MONOCYTOGENES AND YERSINIA ENTEROCOLITICA, STAPHYLOCOCCUS AUREUS, BACILLUS SPP AND PAENIBACILLUS SPP. OUR LABORATORY HAS A WIDE COLLECTION OF BACTERIAL STRAINS ISOLATED FROM VARIOUS FOODS (MEAT, FISH, MINIMALLY PROCESSED AND READY FOR CONSUMPTION, MILK, ETC.). ALL OF THEM HAVE BEEN CHARACTERISED BY DIFFERENT METHODS, BOTH CLASSICAL AND GENOMIC AND PROTEOMIC (MALDI-TOF). Such RELATED SPECIES WITH YOUR SPECIFIC MALDI-TOF SPECTIVES AND GENOMIC SECUCE OF GEN 16 are deposited at the SPECTRABANK DATA BASE (WWW.SPECTRABANK.ORG) as well as in the GENBANK DATA BASE (NCBI) (only the SECUENCE OF GEN 16S rRNA)._x000D_ METODOLOGY AND RESULTS they will allow them to expand the SPECTRABANK DATA BASE (WWW.SPECTRABANK.ORG), developed in our laboratorium and constituting the highest international data base of access (English) / qualifier
point in time: 12 October 2021
Timestamp+2021-10-12T00:00:00Z
Timezone+00:00
CalendarGregorian
Precision1 day
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Revision as of 15:41, 12 October 2021

Project Q3151710 in Spain
Language Label Description Also known as
English
APPLICATION OF ELECTROSPRAY MASS IONISATION SPECTROMETRY FOR RAPID IDENTIFICATION AND QUANTIFICATION OF FOOD PATHOGENS
Project Q3151710 in Spain

    Statements

    country
    Spain
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    EU contribution
    106,480.0 Euro
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    budget
    133,100.0 Euro
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    co-financing rate
    80.0 percent
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    start time
    1 January 2014
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    end time
    30 June 2017
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    beneficiary name (string)
    UNIVERSIDAD DE SANTIAGO DE COMPOSTELA
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    coordinate location

    43°2'46.39"N, 7°28'26.36"W
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    postal code
    27028
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    summary
    EL INCREMENTO DE LAS ENFERMEDADES TRANSMITIDAS POR LOS ALIMENTOS EN LOS ULTIMOS AÑOS, CON UNA ESPECIAL EMERGENCIA DE MICROORGANISMOS PATOGENOS COMO LISTERIA MONOCYTOGENES, SALMONELLA SPP, CAMPYLOBACTER JEJUNI, O ESCHERICHIA COLI, HA AUMENTADO LA PREOCUPACION SOBRE LA SEGURIDAD ALIMENTARIA TANTO ENTRE LAS AUTORIDADES COMO EN EL SECTOR PRODUCTIVO Y ENTRE LOS CONSUMIDORES. LOS PATOGENOS ALIMENTARIOS NO PUEDEN SER ELIMINADOS COMPLETAMENTE DE TODOS LOS ESTADIOS DE LA CADENA ALIMENTARIA. ADEMAS, SEGUN EL TRATAMIENTO SUFRIDO POR EL ALIMENTO, LA MICROBIOTA PRESENTE PUEDE VARIAR. EXISTEN NUMEROSOS PATOGENOS QUE SE PUEDE REQUERIR A LA EMPRESA PROCESADORA QUE ANALICE PARA DETERMINAR LA RELATIVA SEGURIDAD DE SU PRODUCTO. LOS METODOS DE DETECCION E IDENTIFICACION RAPIDA IMPLEMENTADOS EN LA ACTUALIDAD PRESENTAN LA DESVENTAJA DE ESTAR LIMITADOS A UNAS CUANTAS ESPECIES (PCR EN TIEMPO REAL), NECESITAR UN PROCESADO LARGO (SECUENCIACION,¿), NO SER CUANTITATIVOS (SECUENCIACION, MALDI-TOF), NO PERMITIR LA DETECCION EN MEZCLAS COMPLEJAS COMO LOS ALIMENTOS (SECUENCIACION, MALDI-TOF), Y PRACTICAMENTE TODOS ELLOS NECESITAR PREVIAMENTE LA SIEMBRA EN PLACA. ES POR ELLO QUE SE HACE NECESARIA LA IMPLEMENTACION DE METODOLOGIAS QUE PERMITAN LA DETECCION RAPIDA Y PRECISA DE LAS ESPECIES CONTAMINANTES EN EL ALIMENTO ASI COMO LA CUANTIFICACION DIRECTAMENTE EN UNA MUESTRA DE ALIMENTO. ESTE ES EL CASO DE LA METODOLOGIA QUE SE PROPONE EN EL PRESENTE PROYECTO: LA DEFINICION DE PEPTIDOS BIOMARCADORES DE ESPECIES PATOGENAS Y PRODUCTORAS DE AMINAS BIOGENAS EN ALIMENTOS Y LA POSTERIOR IMPLEMENTACION DE LA APLICACION DE DICHOS PEPTIDOS PARA LA DETECCION EN MUESTRAS ALIMENTARIAS. PARA ELLO SE UTILIZARA LA CROMATOGRAFIA LIQUIDA CAPILAR ACOPLADA A ESPECTROMETRIA DE MASAS EN TANDEM EN UN EQUIPO ELECTROSPRAY-TRAMPA IONICA (LC-ESI-IT-MS/MS) Y POSTERIOR ANALISIS MEDIANTE DIFERENTES SOFTWARE Y COMPARACION CON LAS SECUENCIAS AMINOACIDICAS DEPOSITADAS EN BASES DE DATOS._x000D_ PARA LLEVAR A CABO LA DEFINICION DE PEPTIDOS BIOMARCADORES ES NECESARIO DISPONER DE UN AMPLIO NUMERO DE CEPAS BACTERIANAS PARA LLEVAR A CABO EL ANALISIS. LAS ESPECIES BACTERIANAS QUE SE INCLUIRAN EN EL ESTUDIO COMPRENDERAN AQUELLAS QUE SE AISLAN MAS FRECUENTEMENTE EN PRODUCTOS ALIMENTARIOS, SEGUN LO DESCRITO EN LA LITERATURA, E INCLUYEN LA FAMILIA ENTEROBACTERIACEAE, SALMONELLA, SHIGELLA, PROTEUS, ESTAFILOCOCOS COAGULASA-POSITIVOS; CLOSTRIDIUM PERFRINGENS, L. MONOCYTOGENES, BACILLUS CEREUS, Y C. BOTULINUM TIPOS E Y B, BACILLUS CEREUS, B. SUBTILIS Y B. LICHENIFORMIS, VIBRIO PARAHAEMOLYTICUS, CAMPYLOBACTER SPP., CAMPYLOBACTER JEJUNI, LISTERIA MONOCYTOGENES Y YERSINIA ENTEROCOLITICA, STAPHYLOCOCCUS AUREUS, BACILLUS SPP Y PAENIBACILLUS SPP. NUESTRO LABORATORIO DISPONE DE UNA AMPLIA COLECCION DE CEPAS BACTERIANAS AISLADAS DE DIVERSOS ALIMENTOS (CARNICOS, PESCADO, MINIMAMENTE PROCESADO Y LISTOS PARA EL CONSUMO, LACTEOS,¿). TODAS ELLAS HAN SIDO CARACTERIZADAS MEDIANTE DIFERENTES METODOLOGIAS TANTO CLASICAS COMO GENOMICAS Y PROTEOMICAS (MALDI-TOF). DICHA RELACION DE ESPECIES CON SUS CORRESPONDIENTES ESPECTROS DE MALDI-TOF Y SECUENCIA GENOMICA DEL GEN 16S RRNA ESTAN DEPOSITADAS EN LA BASE DE DATOS SPECTRABANK (WWW.SPECTRABANK.ORG) ASI COMO EN LA BASE DE DATOS DEL GENBANK (NCBI) (SOLO LA SECUENCIA DEL GEN 16S RRNA)._x000D_ LA METODOLOGIA Y RESULTADOS OBTENIDOS PERMITIRAN ASIMISMO AMPLIAR LA BASE DE DATOS SPECTRABANK (WWW.SPECTRABANK.ORG), DESARROLLADA EN NUESTRO LABORATORIO Y QUE CONSTITUIRA LA MAYOR BASE DE DATOS INTERNACIONAL DE ACCESO (Spanish)
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    location (string)
    Lugo
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    Identifiers

    Spanish Kohesio ID
    AGL2013-48244-R
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