Development of nanosensory photonic systems for rapid detection of viruses using controlled evolution methods of protein platforms: case SARS-CoV-2 (Q3107859): Difference between revisions
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(Created claim: summary (P836): The current coronovirus pandemic around the world has highlighted the urgent need for rapid and economically efficient virus detection. P. J. Šafárik University in Košice, in cooperation with SAFTRA Photonics s.r.o. and AUSYS s.r.o., proposes to develop a methodology for rapid, selective and highly sensitive detection of SARS-CoV-2 viruses, which will be captured specifically on the surface of photonic nanostructure chips. Within the project, th...) |
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The current coronovirus pandemic around the world has highlighted the urgent need for rapid and economically efficient virus detection. P. J. Šafárik University in Košice, in cooperation with SAFTRA Photonics s.r.o. and AUSYS s.r.o., proposes to develop a methodology for rapid, selective and highly sensitive detection of SARS-CoV-2 viruses, which will be captured specifically on the surface of photonic nanostructure chips. Within the project, the chip surface will be functionalised by nanolayering of molecules of specific proteins developed by the method of controlled evolution of selected protein platforms. The formation of nanolayers of proteins on the detection chip will be carried out by means of controlled specific chemical conjugation to achieve optimal binding efficiency and reproducibility of detection properties. Once a sample of the inactivated virus has been applied to the functionalised chip, the presence of the virus will be detected on the basis of the increased ramanovsky spectrum by surface plasmamones. BioRAMASCOPE will be developed to detect the signal from functionalised chips. BioRAMASCOPE will meet the specific requirements regarding the required detection limits and operational reliability required for the detection of SARS-CoV-2 virus. In parallel lines, using advanced selection methods of protein engineering, we will develop artificial proteins as new biological entities with a new binding function that specialises in the specific binding of inactivated viruses. Based on the combination of binding different epitopes, we plan that this molecular platform will achieve high sensitivity and specificity to various types of viruses, including SARS-CoV-2. This project, which will be implemented in Košice, will lead to scientific publications, “proof of concept” and patent, namely: I) “proof of concept” methodology of selective, rapid, inexpensive and in-situ detection of SARS-CoV-2 with immediate transmission of the result to the relevant database, (ii) functional laboratory prototype of detection system of various types of viruses, including SARS-CoV-2 virus. (English) | |||||||||||||||
| Property / summary: The current coronovirus pandemic around the world has highlighted the urgent need for rapid and economically efficient virus detection. P. J. Šafárik University in Košice, in cooperation with SAFTRA Photonics s.r.o. and AUSYS s.r.o., proposes to develop a methodology for rapid, selective and highly sensitive detection of SARS-CoV-2 viruses, which will be captured specifically on the surface of photonic nanostructure chips. Within the project, the chip surface will be functionalised by nanolayering of molecules of specific proteins developed by the method of controlled evolution of selected protein platforms. The formation of nanolayers of proteins on the detection chip will be carried out by means of controlled specific chemical conjugation to achieve optimal binding efficiency and reproducibility of detection properties. Once a sample of the inactivated virus has been applied to the functionalised chip, the presence of the virus will be detected on the basis of the increased ramanovsky spectrum by surface plasmamones. BioRAMASCOPE will be developed to detect the signal from functionalised chips. BioRAMASCOPE will meet the specific requirements regarding the required detection limits and operational reliability required for the detection of SARS-CoV-2 virus. In parallel lines, using advanced selection methods of protein engineering, we will develop artificial proteins as new biological entities with a new binding function that specialises in the specific binding of inactivated viruses. Based on the combination of binding different epitopes, we plan that this molecular platform will achieve high sensitivity and specificity to various types of viruses, including SARS-CoV-2. This project, which will be implemented in Košice, will lead to scientific publications, “proof of concept” and patent, namely: I) “proof of concept” methodology of selective, rapid, inexpensive and in-situ detection of SARS-CoV-2 with immediate transmission of the result to the relevant database, (ii) functional laboratory prototype of detection system of various types of viruses, including SARS-CoV-2 virus. (English) / rank | |||||||||||||||
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| Property / summary: The current coronovirus pandemic around the world has highlighted the urgent need for rapid and economically efficient virus detection. P. J. Šafárik University in Košice, in cooperation with SAFTRA Photonics s.r.o. and AUSYS s.r.o., proposes to develop a methodology for rapid, selective and highly sensitive detection of SARS-CoV-2 viruses, which will be captured specifically on the surface of photonic nanostructure chips. Within the project, the chip surface will be functionalised by nanolayering of molecules of specific proteins developed by the method of controlled evolution of selected protein platforms. The formation of nanolayers of proteins on the detection chip will be carried out by means of controlled specific chemical conjugation to achieve optimal binding efficiency and reproducibility of detection properties. Once a sample of the inactivated virus has been applied to the functionalised chip, the presence of the virus will be detected on the basis of the increased ramanovsky spectrum by surface plasmamones. BioRAMASCOPE will be developed to detect the signal from functionalised chips. BioRAMASCOPE will meet the specific requirements regarding the required detection limits and operational reliability required for the detection of SARS-CoV-2 virus. In parallel lines, using advanced selection methods of protein engineering, we will develop artificial proteins as new biological entities with a new binding function that specialises in the specific binding of inactivated viruses. Based on the combination of binding different epitopes, we plan that this molecular platform will achieve high sensitivity and specificity to various types of viruses, including SARS-CoV-2. This project, which will be implemented in Košice, will lead to scientific publications, “proof of concept” and patent, namely: I) “proof of concept” methodology of selective, rapid, inexpensive and in-situ detection of SARS-CoV-2 with immediate transmission of the result to the relevant database, (ii) functional laboratory prototype of detection system of various types of viruses, including SARS-CoV-2 virus. (English) / qualifier | |||||||||||||||
point in time: 28 September 2021
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Revision as of 14:41, 28 September 2021
Project Q3107859 in Slovakia
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | Development of nanosensory photonic systems for rapid detection of viruses using controlled evolution methods of protein platforms: case SARS-CoV-2 |
Project Q3107859 in Slovakia |
Statements
1,935,624.64 Euro
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3,060,199.77 Euro
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63.25 percent
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1 January 2021
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6 January 2023
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Univerzita Pavla Jozefa Šafárika v Košiciach
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48°43'20.53"N, 21°15'25.20"E
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Súčasná pandémia koronovírusu vo svete poukázala na naliehavú potrebu rýchlej a ekonomicky efektívnej detekcie vírusov. Univerzita P. J. Šafárika v Košiciach v spolupráci so spoločnosťami SAFTRA Photonics s.r.o. a AUSYS s.r.o, navrhuje vyvinúť metodiku na rýchlu, selektívnu a vysoko citlivú detekciu vírusov SARS-CoV-2, ktoré budú zachytené špecificky na povrch fotonických nanoštruktúrnych čipov. V rámci projektu bude povrch čipu funkcionalizovaný nanovrstvou molekúl špecifických proteínov vyvinutých metódou riadenej evolúcie vybraných proteínových platforiem. Tvorba nanovrstiev proteínov na detekčnom čipe bude prebiehať pomocou kontrolovanej špecifickej chemickej konjugácie, čim dosiahneme optimálnu efektivitu viazania a reprodukovateľnosť detekčných vlastností. Po nanesení vzorky inaktivovaného vírusu na funkcionalizovaný čip bude prítomnosť vírusu detegovaná na základe zosilneného ramanovského spektra povrchovými plazmónmi. Na detekciu signálu z funkcionalizovaných čipov bude vyvinutý detekčný systém bioRAMASCOPE. bioRAMASCOPE bude spĺňať špecifické požiadavky týkajúce sa požadovaných detekčných limitov a spoľahlivosti prevádzky vyžadovaných pre detekciu vírusu SARS-CoV-2. V paralelnej línií budeme pomocou moderných selekčných metód proteínového inžinierstva vyvíjať artificiálne proteíny ako nové biologické entity s novou väzobnou funkciou, ktorá sa špecializuje na špecifické viazanie inaktivovaných vírusov. Na základe kombinácie viazania rôznych epitopov plánujeme, že táto molekulová platforma dosiahne vysokú citlivosť a špecificitu voči rôznym typom vírusov, vrátane SARS-CoV-2. Tento projekt, ktorý bude realizovaný v Košiciach, povedie k vedeckým publikáciám, “proof of concept” a patentu, konkrétne: i) „proof of concept“ metodiky selektívnej, rýchlej, lacnej a in situ vykonateľnej detekcie SARS-CoV-2 s okamžitým zaslaním výsledku do príslušnej databázy, ii) funkčný laboratórny prototyp detekčného systému rôznych typov vírusov, vrátane vírusu SARS-CoV-2. (Slovak)
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The current coronovirus pandemic around the world has highlighted the urgent need for rapid and economically efficient virus detection. P. J. Šafárik University in Košice, in cooperation with SAFTRA Photonics s.r.o. and AUSYS s.r.o., proposes to develop a methodology for rapid, selective and highly sensitive detection of SARS-CoV-2 viruses, which will be captured specifically on the surface of photonic nanostructure chips. Within the project, the chip surface will be functionalised by nanolayering of molecules of specific proteins developed by the method of controlled evolution of selected protein platforms. The formation of nanolayers of proteins on the detection chip will be carried out by means of controlled specific chemical conjugation to achieve optimal binding efficiency and reproducibility of detection properties. Once a sample of the inactivated virus has been applied to the functionalised chip, the presence of the virus will be detected on the basis of the increased ramanovsky spectrum by surface plasmamones. BioRAMASCOPE will be developed to detect the signal from functionalised chips. BioRAMASCOPE will meet the specific requirements regarding the required detection limits and operational reliability required for the detection of SARS-CoV-2 virus. In parallel lines, using advanced selection methods of protein engineering, we will develop artificial proteins as new biological entities with a new binding function that specialises in the specific binding of inactivated viruses. Based on the combination of binding different epitopes, we plan that this molecular platform will achieve high sensitivity and specificity to various types of viruses, including SARS-CoV-2. This project, which will be implemented in Košice, will lead to scientific publications, “proof of concept” and patent, namely: I) “proof of concept” methodology of selective, rapid, inexpensive and in-situ detection of SARS-CoV-2 with immediate transmission of the result to the relevant database, (ii) functional laboratory prototype of detection system of various types of viruses, including SARS-CoV-2 virus. (English)
28 September 2021
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Identifiers
313011AUW6
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