Q3180817 (Q3180817): Difference between revisions
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(Created claim: summary (P836): Multiple myeloma (MM) is a haematological neoplasia with a very low healing rate. Along with genetic alterations, it is increasingly evident that both epigenetic alterations and non-coding RNAs can play an important role in the pathogenesis of MM. The overall objective of this project is to analyse the (hydroxy)methyloma of the complete DNA and to know its role in the regulation of transcriptome, especially of long non-coding RNAs (lncRNAs), det...) |
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Multiple myeloma (MM) is a haematological neoplasia with a very low healing rate. Along with genetic alterations, it is increasingly evident that both epigenetic alterations and non-coding RNAs can play an important role in the pathogenesis of MM. The overall objective of this project is to analyse the (hydroxy)methyloma of the complete DNA and to know its role in the regulation of transcriptome, especially of long non-coding RNAs (lncRNAs), determine whether lncRNAs are involved in the pathogenesis and prognosis of MM, and design new molecules against these lncRNAs that improve the treatment of MM patients. The (hydroxy)methyloma shall be analysed by Bis-seq, TET-seq and methylation arrays and transcriptoma by RNA-seq. The implication of altered expression or methylation in the prognosis of MM will be studied by Q-RT-PCR and pyrossequencing or MSP in a series of 200 samples of MM patients. Of the most altered lncRNAs, their functionality will be analysed in terms of their involvement in the pathogenesis of MM (proliferation, cell cycle, apoptosis) both in vitro and in vivo. Finally we will design ifAptameros or small molecules against the two most relevant lncRNAs in the MM. Through this study in this project we will decipher the (hydroxy)methyloma of the MM, the deregulated lncRNAs and with a clear functionality in the development and prognosis of the MM and finally we will design new strategies aimed at lncRNAs to improve the treatment and quality of life of these patients. (English) | |||||||||||||||
| Property / summary: Multiple myeloma (MM) is a haematological neoplasia with a very low healing rate. Along with genetic alterations, it is increasingly evident that both epigenetic alterations and non-coding RNAs can play an important role in the pathogenesis of MM. The overall objective of this project is to analyse the (hydroxy)methyloma of the complete DNA and to know its role in the regulation of transcriptome, especially of long non-coding RNAs (lncRNAs), determine whether lncRNAs are involved in the pathogenesis and prognosis of MM, and design new molecules against these lncRNAs that improve the treatment of MM patients. The (hydroxy)methyloma shall be analysed by Bis-seq, TET-seq and methylation arrays and transcriptoma by RNA-seq. The implication of altered expression or methylation in the prognosis of MM will be studied by Q-RT-PCR and pyrossequencing or MSP in a series of 200 samples of MM patients. Of the most altered lncRNAs, their functionality will be analysed in terms of their involvement in the pathogenesis of MM (proliferation, cell cycle, apoptosis) both in vitro and in vivo. Finally we will design ifAptameros or small molecules against the two most relevant lncRNAs in the MM. Through this study in this project we will decipher the (hydroxy)methyloma of the MM, the deregulated lncRNAs and with a clear functionality in the development and prognosis of the MM and finally we will design new strategies aimed at lncRNAs to improve the treatment and quality of life of these patients. (English) / rank | |||||||||||||||
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| Property / summary: Multiple myeloma (MM) is a haematological neoplasia with a very low healing rate. Along with genetic alterations, it is increasingly evident that both epigenetic alterations and non-coding RNAs can play an important role in the pathogenesis of MM. The overall objective of this project is to analyse the (hydroxy)methyloma of the complete DNA and to know its role in the regulation of transcriptome, especially of long non-coding RNAs (lncRNAs), determine whether lncRNAs are involved in the pathogenesis and prognosis of MM, and design new molecules against these lncRNAs that improve the treatment of MM patients. The (hydroxy)methyloma shall be analysed by Bis-seq, TET-seq and methylation arrays and transcriptoma by RNA-seq. The implication of altered expression or methylation in the prognosis of MM will be studied by Q-RT-PCR and pyrossequencing or MSP in a series of 200 samples of MM patients. Of the most altered lncRNAs, their functionality will be analysed in terms of their involvement in the pathogenesis of MM (proliferation, cell cycle, apoptosis) both in vitro and in vivo. Finally we will design ifAptameros or small molecules against the two most relevant lncRNAs in the MM. Through this study in this project we will decipher the (hydroxy)methyloma of the MM, the deregulated lncRNAs and with a clear functionality in the development and prognosis of the MM and finally we will design new strategies aimed at lncRNAs to improve the treatment and quality of life of these patients. (English) / qualifier | |||||||||||||||
point in time: 12 October 2021
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Revision as of 19:24, 12 October 2021
Project Q3180817 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | No label defined |
Project Q3180817 in Spain |
Statements
28,750.0 Euro
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57,500.0 Euro
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50.0 percent
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1 January 2014
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31 March 2017
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FUNDACION PARA LA INVESTIGACION MEDICA APLICADA
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42°49'6.42"N, 1°38'39.34"W
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31201
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El Mieloma múltiple (MM) es una neoplasia hematológica con una muy baja tasa de curación. Junto a las alteraciones genéticas, cada vez es más evidente que tanto las alteraciones epigenéticas como los RNAs no-codificantes pueden jugar un papel importante en la patogénesis del MM. El objetivo general de este proyecto es analizar el (hidroxi)metiloma del DNA completo y conocer su papel en la regulación del transcriptoma, especialmente de los RNAs largos no-codificantes (lncRNAs), determinar si los lncRNAs están involucrados en la patogénesis y el pronóstico del MM y diseñar nuevas moléculas frente a estos lncRNAs que mejoren el tratamiento de los pacientes con MM. El (hidroxi)metiloma se analizará mediante Bis-seq, TET-seq y arrays de metilación y el transcriptoma mediante RNA-seq. La implicación de la expresión alterada o la metilación en el pronóstico del MM se estudiará mediante Q-RT-PCR y pirosecuenciación o MSP en una serie de 200 muestras de pacientes MM. De los lncRNAs que presenten una mayor alteración se analizará su funcionalidad en cuanto a su implicación en la patogénesis del MM (proliferación, ciclo celular, apoptosis) tanto in vitro como in vivo. Por último diseñaremos siAptameros o pequeñas moléculas frente a los dos lncRNAs más relevantes en el MM. Mediante este estudio en este proyecto descifraremos el (hidroxi)metiloma del MM, los lncRNAs desregulados y con una clara funcionalidad en el desarrollo y pronóstico del MM y por último diseñaremos nuevas estrategias dirigidas a los lncRNAs para mejorar el tratamiento y la calidad de vida de estos pacientes. (Spanish)
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Multiple myeloma (MM) is a haematological neoplasia with a very low healing rate. Along with genetic alterations, it is increasingly evident that both epigenetic alterations and non-coding RNAs can play an important role in the pathogenesis of MM. The overall objective of this project is to analyse the (hydroxy)methyloma of the complete DNA and to know its role in the regulation of transcriptome, especially of long non-coding RNAs (lncRNAs), determine whether lncRNAs are involved in the pathogenesis and prognosis of MM, and design new molecules against these lncRNAs that improve the treatment of MM patients. The (hydroxy)methyloma shall be analysed by Bis-seq, TET-seq and methylation arrays and transcriptoma by RNA-seq. The implication of altered expression or methylation in the prognosis of MM will be studied by Q-RT-PCR and pyrossequencing or MSP in a series of 200 samples of MM patients. Of the most altered lncRNAs, their functionality will be analysed in terms of their involvement in the pathogenesis of MM (proliferation, cell cycle, apoptosis) both in vitro and in vivo. Finally we will design ifAptameros or small molecules against the two most relevant lncRNAs in the MM. Through this study in this project we will decipher the (hydroxy)methyloma of the MM, the deregulated lncRNAs and with a clear functionality in the development and prognosis of the MM and finally we will design new strategies aimed at lncRNAs to improve the treatment and quality of life of these patients. (English)
12 October 2021
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Pamplona/Iruña
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Identifiers
PI13_01469
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