Differential genetic expression analysis in organisms exposed to different types of environmental stress. (Q3177933): Difference between revisions
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(Changed label, description and/or aliases in en: translated_label) |
(Removed claim: summary (P836): A microarrays scan is requested for differential gene expression analysis (transcriptomics). This technique allows detecting the differential expression of thousands of genes or DNA fragments simultaneously in individuals subject to different conditions (environmental or experimental). Alteration of gene expression is an early, rapid and sensitive means of detecting the response to stress. By simultaneously analysing thousands of genes involve...) |
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| Property / summary: A microarrays scan is requested for differential gene expression analysis (transcriptomics). This technique allows detecting the differential expression of thousands of genes or DNA fragments simultaneously in individuals subject to different conditions (environmental or experimental). Alteration of gene expression is an early, rapid and sensitive means of detecting the response to stress. By simultaneously analysing thousands of genes involved in different networks or metabolic pathways of the affected organism, it will be possible to identify modes of action (MOAS) specific to the different contaminants. The subsequent integration of data on concentration levels, bioaccumulation and molecular effects and at a higher level of organisation through bioinformatics will allow the attribution of biological effects and the development of new environmental risk assessment (ERAs) strategies based on the modelling and prediction of toxicities for other compounds of similar structure not studied. Thus, the aim is to reduce practical and ethical conflicts about in vivo experimentation in the future, as proposed in different European and international reports (ECETOC, OECD, US EPA). In addition, the requested equipment can be used for any differential gene expression study, such as molecular effects caused by temperature increase, pH variation or dissolved oxygen related to global change, among others. (English) / rank | |||||||||||||||
| Property / summary: A microarrays scan is requested for differential gene expression analysis (transcriptomics). This technique allows detecting the differential expression of thousands of genes or DNA fragments simultaneously in individuals subject to different conditions (environmental or experimental). Alteration of gene expression is an early, rapid and sensitive means of detecting the response to stress. By simultaneously analysing thousands of genes involved in different networks or metabolic pathways of the affected organism, it will be possible to identify modes of action (MOAS) specific to the different contaminants. The subsequent integration of data on concentration levels, bioaccumulation and molecular effects and at a higher level of organisation through bioinformatics will allow the attribution of biological effects and the development of new environmental risk assessment (ERAs) strategies based on the modelling and prediction of toxicities for other compounds of similar structure not studied. Thus, the aim is to reduce practical and ethical conflicts about in vivo experimentation in the future, as proposed in different European and international reports (ECETOC, OECD, US EPA). In addition, the requested equipment can be used for any differential gene expression study, such as molecular effects caused by temperature increase, pH variation or dissolved oxygen related to global change, among others. (English) / qualifier | |||||||||||||||
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Revision as of 19:04, 12 October 2021
Project Q3177933 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | Differential genetic expression analysis in organisms exposed to different types of environmental stress. |
Project Q3177933 in Spain |
Statements
107,258.4 Euro
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134,073.0 Euro
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80.0 percent
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1 January 2016
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30 June 2018
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UNIVERSIDAD DE CADIZ
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36°31'43.28"N, 6°11'24.79"W
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11028
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Se solicita un escaner de microarrays para el análisis de expresión génica diferencial (transcriptómica). Esta técnica permite detectar la expresión diferencial de miles de genes o fragmentos de ADN simultáneamente en individuos sometidos a distintas condiciones (ambientales o experimentales). La alteración de la expresión génica es un medio temprano, rápido y sensible para la detección de la respuesta al estrés. Al analizar simultáneamente miles de genes involucrados en diferentes redes o rutas metabólicas del organismo afectado, será posible identificar modos de acción (MoAs) específicos de los diferentes contaminantes. La posterior integración de datos de niveles de concentración, bioacumulación y efectos moleculares y a mayor nivel de organización mediante métodos bioinformáticos permitirá atribuir efectos biológicos y el desarrollo de nuevas estrategias de evaluación del riesgo ambiental (ERAs) basadas en la modelización y predicción de toxicidades para otros compuestos de estructura similar no estudiados. Así, se pretende reducir en un futuro conflictos prácticos y éticos acerca de la experimentación in vivo, tal como se propone en diferentes informes europeos e internacionales (ECETOC, OECD, US EPA). Además, el equipo solicitado se puede emplear para cualquier estudio de expresión génica diferencial, como p. ej. los efectos moleculares producidos por el aumento de temperatura, variación de pH o oxígeno disuelto relacionados con el cambio global, entre otros. (Spanish)
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Puerto Real
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Identifiers
UNCA15-CE-3375
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