ECOLOGY OF MARINE MICROORGANISMS: BIODIVERSITY, GENOMIC AND METAGENOMIC (Q3165618): Difference between revisions
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(Removed claim: summary (P836): THE OCEANS CONSTITUTE THE LARGEST ECOSYSTEM ON EARTH AND ONE OF THE MOST UNKNOWN. THE ACTIVITIES OF MARINE MICROORGANISMS REGULATE BIOGEOCHEMICAL CYCLES AND HAVE A MAJOR INFLUENCE ON CLIMATE CONTROL. MOREOVER, ALMOST HALF OF THE HUMAN POPULATION LIVES NEAR THE SEA AND, THEREFORE, HUMANITY HAS A STRONG IMPACT ON THE OCEANS AND, ON THE CONTRARY, DEPENDS HEAVILY ON THEIR SERVICES. FOR THESE REASONS, MARINE RESEARCH HAS BEEN CONSIDERED ONE OF THE...) |
(Created claim: summary (P836): THE OCEANS CONSTITUTE THE LARGEST ECOSYSTEM ON EARTH AND ONE OF THE MOST UNKNOWN. THE ACTIVITIES OF MARINE MICROORGANISMS REGULATE BIOGEOCHEMICAL CYCLES AND HAVE A MAJOR INFLUENCE ON CLIMATE CONTROL. MOREOVER, ALMOST HALF OF THE HUMAN POPULATION LIVES NEAR THE SEA AND, THEREFORE, HUMANITY HAS A STRONG IMPACT ON THE OCEANS AND, ON THE CONTRARY, DEPENDS HEAVILY ON THEIR SERVICES. FOR THESE REASONS, MARINE RESEARCH HAS BEEN CONSIDERED ONE OF THE SO...) |
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THE OCEANS CONSTITUTE THE LARGEST ECOSYSTEM ON EARTH AND ONE OF THE MOST UNKNOWN. THE ACTIVITIES OF MARINE MICROORGANISMS REGULATE BIOGEOCHEMICAL CYCLES AND HAVE A MAJOR INFLUENCE ON CLIMATE CONTROL. MOREOVER, ALMOST HALF OF THE HUMAN POPULATION LIVES NEAR THE SEA AND, THEREFORE, HUMANITY HAS A STRONG IMPACT ON THE OCEANS AND, ON THE CONTRARY, DEPENDS HEAVILY ON THEIR SERVICES. FOR THESE REASONS, MARINE RESEARCH HAS BEEN CONSIDERED ONE OF THE SOCIAL CHALLENGES IN THE HORIZON 2020 PROGRAMME. IN THE PRESENT PROJECT WE PLAN TO STUDY SOME ASPECTS OF THE DIVERSITY, STRUCTURE AND FUNCTIONAL CAPABILITIES OF MARINE BACTERIAL COMMUNITIES. Our PARTY POINT is that the Availability of an Enormous Quantity of OCEAN SECUTIONS opens a VENTANA OF OPPORTUNITY to resolve questions that could not be considered before the advent of Genome and METAGENOMIC._x000D_ COORDINA PROJECT THREE PROJECT WITH COMMUNITY INTERESTS WITH DIFFERENT approaches. THE FIRST SUB-PROJECT WILL FOCUS ON THE DIVERSITY OF BACTERIAL COMMUNITIES. FOR THIS PURPOSE, LABEL SEQUENCES (TAGS) OF THE 16S RDNA GENE WILL BE USED TO EXPLORE THE BIOSPHERE OF RARE MICROORGANISMS: THE SET OF A VERY HIGH NUMBER OF SPECIES WITH EXTRAORDINARILY LOW ABUNDANCES. WE WILL TRY TO DETERMINE THE TOTAL NUMBER OF BACTERIAL TAXA IN THE OCEANS AND WE WILL DEVELOP A METHOD TO ANALYSE THE RANGE-ABUNDANCE CURVES THAT WE HOPE WILL REVEAL ECOLOGICAL PROPERTIES OF BACTERIAL COMMUNITIES. THE SECOND SUB-PROJECT WILL ANALYSE COMMUNITIES USING METAGENOMICA AND METATRANSCRIPTOMICA. A PART OF THIS SP2 OVERLAPS WITH THE SP1 TO COMPARE THE DIVERSITY REVEALED BY THE LABELED SEQUENCES WITH THE DERIVATIVE OF THE METAGENOMES. AN IMPORTANT COMPONENT OF THIS SP2 WILL BE THE COMPARISON OF COMMUNITIES BASED ON THE PHYLOGENY AND THE FUNCTIONS IT CONTAINS. FROM THE COEXISTENCE IN DIFFERENT COMMUNITIES, NETWORKS OF ASSOCIATION BETWEEN BACTERIAL TAXA WILL BE ESTABLISHED IN ORDER, AGAIN, TO DERIVE PROPERTIES OF THE COMMUNITIES. THE THIRD SUBPROJECT WILL PAY ATTENTION TO A MODEL GROUP OF MARINE BACTERIA, POLARIBACTER, A GENUS WITHIN THE LARGE GROUP OF BACTEROIDS. THIS GENUS IS RELATIVELY ABUNDANT IN OCEANS, PARTICULARLY IN POLAR WATERS. SOME OF ITS MEMBERS CONTAIN THE PROTEORRODOPSIN GENE, A PROTEIN THAT ALLOWS BACTERIA TO USE LIGHT ENERGY. SP3 WILL ANALYSE THE REGULATION AND RELATIONSHIP OF THIS GROUP OF BACTERIA TO LIGHT. MEMBERS OF THE GENUS WILL BE ISOLATED IN PURE CULTURE AND THEIR GENOMES WILL BE SEQUENCED. AMPLIFIED GENOMES WILL ALSO BE OBTAINED FROM INDIVIDUAL CELLS (SAGS). THESE GENOMES WILL BE USED TO STUDY POLARIBACTER’S CAPABILITIES AND COMPARED TO BACTEROID SEQUENCES DERIVED FROM METAGENOMAS IN SP2. FINALLY, EXPERIMENTS WILL BE CARRIED OUT TO DETERMINE THE GENIC EXPRESSION OF THESE BACTERIA UNDER DIFFERENT DARK LIGHT CONDITIONS AND THE RESULTS WILL BE COMPARED WITH THOSE OBTAINED FROM METATRANSCRIPTOMS IN THE SP2. The OBJECTIVE OF THIS SP3 IS COMPRENDER AS THE CONTROL OF METABOLISM AND THE MANAGEMENT OF METABOLISM IN PRESENCE OF LIGHT IN A HETEROTROFICAL BACTERIA._x000D_ RESULTS have a SIGNIFICATIVE SCIENTIFIC IMPACT IN THE CHANGE OF MICROBIAN Oceanografia and we hope that they open new paths to understand the ecology of the oceans. (English) | |||||||||||||||
| Property / summary: THE OCEANS CONSTITUTE THE LARGEST ECOSYSTEM ON EARTH AND ONE OF THE MOST UNKNOWN. THE ACTIVITIES OF MARINE MICROORGANISMS REGULATE BIOGEOCHEMICAL CYCLES AND HAVE A MAJOR INFLUENCE ON CLIMATE CONTROL. MOREOVER, ALMOST HALF OF THE HUMAN POPULATION LIVES NEAR THE SEA AND, THEREFORE, HUMANITY HAS A STRONG IMPACT ON THE OCEANS AND, ON THE CONTRARY, DEPENDS HEAVILY ON THEIR SERVICES. FOR THESE REASONS, MARINE RESEARCH HAS BEEN CONSIDERED ONE OF THE SOCIAL CHALLENGES IN THE HORIZON 2020 PROGRAMME. IN THE PRESENT PROJECT WE PLAN TO STUDY SOME ASPECTS OF THE DIVERSITY, STRUCTURE AND FUNCTIONAL CAPABILITIES OF MARINE BACTERIAL COMMUNITIES. Our PARTY POINT is that the Availability of an Enormous Quantity of OCEAN SECUTIONS opens a VENTANA OF OPPORTUNITY to resolve questions that could not be considered before the advent of Genome and METAGENOMIC._x000D_ COORDINA PROJECT THREE PROJECT WITH COMMUNITY INTERESTS WITH DIFFERENT approaches. THE FIRST SUB-PROJECT WILL FOCUS ON THE DIVERSITY OF BACTERIAL COMMUNITIES. FOR THIS PURPOSE, LABEL SEQUENCES (TAGS) OF THE 16S RDNA GENE WILL BE USED TO EXPLORE THE BIOSPHERE OF RARE MICROORGANISMS: THE SET OF A VERY HIGH NUMBER OF SPECIES WITH EXTRAORDINARILY LOW ABUNDANCES. WE WILL TRY TO DETERMINE THE TOTAL NUMBER OF BACTERIAL TAXA IN THE OCEANS AND WE WILL DEVELOP A METHOD TO ANALYSE THE RANGE-ABUNDANCE CURVES THAT WE HOPE WILL REVEAL ECOLOGICAL PROPERTIES OF BACTERIAL COMMUNITIES. THE SECOND SUB-PROJECT WILL ANALYSE COMMUNITIES USING METAGENOMICA AND METATRANSCRIPTOMICA. A PART OF THIS SP2 OVERLAPS WITH THE SP1 TO COMPARE THE DIVERSITY REVEALED BY THE LABELED SEQUENCES WITH THE DERIVATIVE OF THE METAGENOMES. AN IMPORTANT COMPONENT OF THIS SP2 WILL BE THE COMPARISON OF COMMUNITIES BASED ON THE PHYLOGENY AND THE FUNCTIONS IT CONTAINS. FROM THE COEXISTENCE IN DIFFERENT COMMUNITIES, NETWORKS OF ASSOCIATION BETWEEN BACTERIAL TAXA WILL BE ESTABLISHED IN ORDER, AGAIN, TO DERIVE PROPERTIES OF THE COMMUNITIES. THE THIRD SUBPROJECT WILL PAY ATTENTION TO A MODEL GROUP OF MARINE BACTERIA, POLARIBACTER, A GENUS WITHIN THE LARGE GROUP OF BACTEROIDS. THIS GENUS IS RELATIVELY ABUNDANT IN OCEANS, PARTICULARLY IN POLAR WATERS. SOME OF ITS MEMBERS CONTAIN THE PROTEORRODOPSIN GENE, A PROTEIN THAT ALLOWS BACTERIA TO USE LIGHT ENERGY. SP3 WILL ANALYSE THE REGULATION AND RELATIONSHIP OF THIS GROUP OF BACTERIA TO LIGHT. MEMBERS OF THE GENUS WILL BE ISOLATED IN PURE CULTURE AND THEIR GENOMES WILL BE SEQUENCED. AMPLIFIED GENOMES WILL ALSO BE OBTAINED FROM INDIVIDUAL CELLS (SAGS). THESE GENOMES WILL BE USED TO STUDY POLARIBACTER’S CAPABILITIES AND COMPARED TO BACTEROID SEQUENCES DERIVED FROM METAGENOMAS IN SP2. FINALLY, EXPERIMENTS WILL BE CARRIED OUT TO DETERMINE THE GENIC EXPRESSION OF THESE BACTERIA UNDER DIFFERENT DARK LIGHT CONDITIONS AND THE RESULTS WILL BE COMPARED WITH THOSE OBTAINED FROM METATRANSCRIPTOMS IN THE SP2. The OBJECTIVE OF THIS SP3 IS COMPRENDER AS THE CONTROL OF METABOLISM AND THE MANAGEMENT OF METABOLISM IN PRESENCE OF LIGHT IN A HETEROTROFICAL BACTERIA._x000D_ RESULTS have a SIGNIFICATIVE SCIENTIFIC IMPACT IN THE CHANGE OF MICROBIAN Oceanografia and we hope that they open new paths to understand the ecology of the oceans. (English) / rank | |||||||||||||||
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| Property / summary: THE OCEANS CONSTITUTE THE LARGEST ECOSYSTEM ON EARTH AND ONE OF THE MOST UNKNOWN. THE ACTIVITIES OF MARINE MICROORGANISMS REGULATE BIOGEOCHEMICAL CYCLES AND HAVE A MAJOR INFLUENCE ON CLIMATE CONTROL. MOREOVER, ALMOST HALF OF THE HUMAN POPULATION LIVES NEAR THE SEA AND, THEREFORE, HUMANITY HAS A STRONG IMPACT ON THE OCEANS AND, ON THE CONTRARY, DEPENDS HEAVILY ON THEIR SERVICES. FOR THESE REASONS, MARINE RESEARCH HAS BEEN CONSIDERED ONE OF THE SOCIAL CHALLENGES IN THE HORIZON 2020 PROGRAMME. IN THE PRESENT PROJECT WE PLAN TO STUDY SOME ASPECTS OF THE DIVERSITY, STRUCTURE AND FUNCTIONAL CAPABILITIES OF MARINE BACTERIAL COMMUNITIES. Our PARTY POINT is that the Availability of an Enormous Quantity of OCEAN SECUTIONS opens a VENTANA OF OPPORTUNITY to resolve questions that could not be considered before the advent of Genome and METAGENOMIC._x000D_ COORDINA PROJECT THREE PROJECT WITH COMMUNITY INTERESTS WITH DIFFERENT approaches. THE FIRST SUB-PROJECT WILL FOCUS ON THE DIVERSITY OF BACTERIAL COMMUNITIES. FOR THIS PURPOSE, LABEL SEQUENCES (TAGS) OF THE 16S RDNA GENE WILL BE USED TO EXPLORE THE BIOSPHERE OF RARE MICROORGANISMS: THE SET OF A VERY HIGH NUMBER OF SPECIES WITH EXTRAORDINARILY LOW ABUNDANCES. WE WILL TRY TO DETERMINE THE TOTAL NUMBER OF BACTERIAL TAXA IN THE OCEANS AND WE WILL DEVELOP A METHOD TO ANALYSE THE RANGE-ABUNDANCE CURVES THAT WE HOPE WILL REVEAL ECOLOGICAL PROPERTIES OF BACTERIAL COMMUNITIES. THE SECOND SUB-PROJECT WILL ANALYSE COMMUNITIES USING METAGENOMICA AND METATRANSCRIPTOMICA. A PART OF THIS SP2 OVERLAPS WITH THE SP1 TO COMPARE THE DIVERSITY REVEALED BY THE LABELED SEQUENCES WITH THE DERIVATIVE OF THE METAGENOMES. AN IMPORTANT COMPONENT OF THIS SP2 WILL BE THE COMPARISON OF COMMUNITIES BASED ON THE PHYLOGENY AND THE FUNCTIONS IT CONTAINS. FROM THE COEXISTENCE IN DIFFERENT COMMUNITIES, NETWORKS OF ASSOCIATION BETWEEN BACTERIAL TAXA WILL BE ESTABLISHED IN ORDER, AGAIN, TO DERIVE PROPERTIES OF THE COMMUNITIES. THE THIRD SUBPROJECT WILL PAY ATTENTION TO A MODEL GROUP OF MARINE BACTERIA, POLARIBACTER, A GENUS WITHIN THE LARGE GROUP OF BACTEROIDS. THIS GENUS IS RELATIVELY ABUNDANT IN OCEANS, PARTICULARLY IN POLAR WATERS. SOME OF ITS MEMBERS CONTAIN THE PROTEORRODOPSIN GENE, A PROTEIN THAT ALLOWS BACTERIA TO USE LIGHT ENERGY. SP3 WILL ANALYSE THE REGULATION AND RELATIONSHIP OF THIS GROUP OF BACTERIA TO LIGHT. MEMBERS OF THE GENUS WILL BE ISOLATED IN PURE CULTURE AND THEIR GENOMES WILL BE SEQUENCED. AMPLIFIED GENOMES WILL ALSO BE OBTAINED FROM INDIVIDUAL CELLS (SAGS). THESE GENOMES WILL BE USED TO STUDY POLARIBACTER’S CAPABILITIES AND COMPARED TO BACTEROID SEQUENCES DERIVED FROM METAGENOMAS IN SP2. FINALLY, EXPERIMENTS WILL BE CARRIED OUT TO DETERMINE THE GENIC EXPRESSION OF THESE BACTERIA UNDER DIFFERENT DARK LIGHT CONDITIONS AND THE RESULTS WILL BE COMPARED WITH THOSE OBTAINED FROM METATRANSCRIPTOMS IN THE SP2. The OBJECTIVE OF THIS SP3 IS COMPRENDER AS THE CONTROL OF METABOLISM AND THE MANAGEMENT OF METABOLISM IN PRESENCE OF LIGHT IN A HETEROTROFICAL BACTERIA._x000D_ RESULTS have a SIGNIFICATIVE SCIENTIFIC IMPACT IN THE CHANGE OF MICROBIAN Oceanografia and we hope that they open new paths to understand the ecology of the oceans. (English) / qualifier | |||||||||||||||
point in time: 12 October 2021
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Revision as of 17:13, 12 October 2021
Project Q3165618 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | ECOLOGY OF MARINE MICROORGANISMS: BIODIVERSITY, GENOMIC AND METAGENOMIC |
Project Q3165618 in Spain |
Statements
92,565.0 Euro
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108,900.0 Euro
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85.0 percent
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1 January 2014
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31 December 2017
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UNIVERSIDAD DE LA LAGUNA
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28°29'8.77"N, 16°18'57.38"W
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38023
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LOS OCEANOS CONSTITUYEN EL ECOSISTEMA MAS GRANDE DE LA TIERRA Y UNO DE LOS MAS DESCONOCIDOS. LAS ACTIVIDADES DE LOS MICROORGANISMOS MARINOS REGULAN LOS CICLOS BIOGEOQUIMICOS Y TIENEN UNA GRAN INFLUENCIA EN EL CONTROL DE CLIMA. ADEMAS, CASI LA MITAD DE LA POBLACION HUMANA VIVE CERCA DEL MAR Y, POR TANTO, LA HUMANIDAD EJERCE UN FUERTE IMPACTO SOBRE LOS OCEANOS Y, AL CONTRARIO, DEPENDE EN GRAN MEDIDA DE SUS SERVICIOS. POR ESTAS RAZONES LA INVESTIGACION MARINA HA SIDO CONSIDERADA UNO DE LOS RETOS SOCIALES EN EL PROGRAMA HORIZONTE 2020. EN EL PROYECTO PRESENTE PLANEAMOS ESTUDIAR ALGUNOS ASPECTOS DE LA DIVERSIDAD, LA ESTRUCTURA Y LAS CAPACIDADES FUNCIONALES DE LAS COMUNIDADES BACTERIANAS MARINAS. NUESTRO PUNTO DE PARTIDA ES QUE LA DISPONIBILIDAD DE UNA CANTIDAD ENORME DE SECUENCIAS DE LOS OCEANOS ABRE UNA VENTANA DE OPORTUNIDAD PARA RESOLVER CUESTIONES QUE NO PODRIA SER ANALIZADAS ANTES DEL ADVENIMIENTO DE LA GENOMICA Y LA METAGENOMICA._x000D_ EL PROYECTO COORDINA TRES SUBPROYECTOS CON INTERESES COMUNES PERO CON APROXIMACIONES DIFERENTES. EL PRIMER SUBPROYECTO SE DEDICARA A LA DIVERSIDAD DE LAS COMUNIDADES BACTERIANAS. PARA ESTE FIN, SE UTILIZARAN SECUENCIAS ETIQUETA (TAGS) DEL GEN 16S RDNA PARA EXPLORAR LA BIOSFERA DE LOS MICROORGANISMOS RAROS: EL CONJUNTO DE UN NUMERO MUY ELEVADO DE ESPECIES CON ABUNDANCIAS EXTRAORDINARIAMENTE BAJAS. INTENTAREMOS DETERMINAR EL NUMERO TOTAL DE TAXONES BACTERIANOS EN LOS OCEANOS Y PONDREMOS A PUNTO UN METODO PARA ANALIZAR LAS CURVAS RANGO-ABUNDANCIA QUE ESPERAMOS REVELARA PROPIEDADES ECOLOGICAS DE LAS COMUNIDADES BACTERIANAS. EL SEGUNDO SUBPROYECTO ANALIZARA LAS COMUNIDADES UTILIZANDO LA METAGENOMICA Y LA METATRANSCRIPTOMICA. UNA PARTE DE ESTE SP2 SE SOLAPARA CON EL SP1 PARA COMPARAR LA DIVERSIDAD REVELADA POR LAS SECUENCIAS ETIQUETA CON LA DERIVADA DE LOS METAGENOMAS. UN COMPONENTE IMPORTANTE DE ESTE SP2 SERA LA COMPARACION DE COMUNIDADES BASANDOSE EN LA FILOGENIA Y EN LAS FUNCIONES QUE CONTIENE. A PARTIR DE LA COEXISTENCIA EN DISTINTAS COMUNIDADES, SE ESTABLECERAN REDES DE ASOCIACION ENTRE TAXONES BACTERIANOS CON EL FIN, NUEVAMENTE, DE DERIVAR PROPIEDADES DE LAS COMUNIDADES. EL TERCER SUBPROYECTO PRESTARA ATENCION A UN GRUPO MODELO DE BACTERIAS MARINAS, POLARIBACTER, UN GENERO DENTRO DEL GRAN GRUPO DE LOS BACTEROIDETES. ESTE GENERO ES RELATIVAMENTE ABUNDANTE EN LOS OCEANOS, PARTICULARMENTE EN AGUAS POLARES. ALGUNOS DE SUS MIEMBROS CONTIENEN EL GEN DE LA PROTEORRODOPSINA, UNA PROTEINA QUE PERMITE A LAS BACTERIAS UTILIZAR ENERGIA DE LA LUZ. SP3 ANALIZARA LA REGULACION Y LA RELACION DE ESTE GRUPO DE BACTERIAS CON LA LUZ. SE AISLARAN MIEMBROS DEL GENERO EN CULTIVO PURO Y SE SECUENCIARAN SUS GENOMAS. TAMBIEN SE OBTENDRAN GENOMAS AMPLIFICADOS A PARTIR DE CELULAS INDIVIDUALES (SAGS). ESTOS GENOMAS SE UTILIZARAN PARA ESTUDIAR LAS CAPACIDADES DE POLARIBACTER Y SE COMPARARAN CON LAS SECUENCIAS DE BACTEROIDETES DERIVADAS DE METAGENOMAS EN EL SP2. FINALMENTE, SE LLEVARAN A CABO EXPERIMENTOS PARA DETERMINAR LA EXPRESION GENICA DE ESTAS BACTERIAS BAJO DIFERENTES CONDICIONES DE LUZ OSCURIDAD Y LOS RESULTADOS SE COMPARARAN CON LOS OBTENIDOS A PARTIR DE METATRANSCRIPTOMAS EN EL SP2. EL OBJETIVO DE ESTE SP3 ES COMPRENDER COMO FUNCIONA EL CONTROL DEL METABOLISMO Y LA GESTION DEL METABOLISMO EN PRESENCIA DE LA LUZ EN UNA BACTERIA HETEROTROFICA._x000D_ LOS RESULTADOS TENDRAN UN IMPACTO CIENTIFICO SIGNIFICATIVO EN EL CAMPO DE LA OCEANOGRAFIA MICROBIANA Y ESPERAMOS QUE ABRAN NUEVAS VIAS PARA COMPRENDER LA ECOLOGIA DE LOS OCEANOS. (Spanish)
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THE OCEANS CONSTITUTE THE LARGEST ECOSYSTEM ON EARTH AND ONE OF THE MOST UNKNOWN. THE ACTIVITIES OF MARINE MICROORGANISMS REGULATE BIOGEOCHEMICAL CYCLES AND HAVE A MAJOR INFLUENCE ON CLIMATE CONTROL. MOREOVER, ALMOST HALF OF THE HUMAN POPULATION LIVES NEAR THE SEA AND, THEREFORE, HUMANITY HAS A STRONG IMPACT ON THE OCEANS AND, ON THE CONTRARY, DEPENDS HEAVILY ON THEIR SERVICES. FOR THESE REASONS, MARINE RESEARCH HAS BEEN CONSIDERED ONE OF THE SOCIAL CHALLENGES IN THE HORIZON 2020 PROGRAMME. IN THE PRESENT PROJECT WE PLAN TO STUDY SOME ASPECTS OF THE DIVERSITY, STRUCTURE AND FUNCTIONAL CAPABILITIES OF MARINE BACTERIAL COMMUNITIES. Our PARTY POINT is that the Availability of an Enormous Quantity of OCEAN SECUTIONS opens a VENTANA OF OPPORTUNITY to resolve questions that could not be considered before the advent of Genome and METAGENOMIC._x000D_ COORDINA PROJECT THREE PROJECT WITH COMMUNITY INTERESTS WITH DIFFERENT approaches. THE FIRST SUB-PROJECT WILL FOCUS ON THE DIVERSITY OF BACTERIAL COMMUNITIES. FOR THIS PURPOSE, LABEL SEQUENCES (TAGS) OF THE 16S RDNA GENE WILL BE USED TO EXPLORE THE BIOSPHERE OF RARE MICROORGANISMS: THE SET OF A VERY HIGH NUMBER OF SPECIES WITH EXTRAORDINARILY LOW ABUNDANCES. WE WILL TRY TO DETERMINE THE TOTAL NUMBER OF BACTERIAL TAXA IN THE OCEANS AND WE WILL DEVELOP A METHOD TO ANALYSE THE RANGE-ABUNDANCE CURVES THAT WE HOPE WILL REVEAL ECOLOGICAL PROPERTIES OF BACTERIAL COMMUNITIES. THE SECOND SUB-PROJECT WILL ANALYSE COMMUNITIES USING METAGENOMICA AND METATRANSCRIPTOMICA. A PART OF THIS SP2 OVERLAPS WITH THE SP1 TO COMPARE THE DIVERSITY REVEALED BY THE LABELED SEQUENCES WITH THE DERIVATIVE OF THE METAGENOMES. AN IMPORTANT COMPONENT OF THIS SP2 WILL BE THE COMPARISON OF COMMUNITIES BASED ON THE PHYLOGENY AND THE FUNCTIONS IT CONTAINS. FROM THE COEXISTENCE IN DIFFERENT COMMUNITIES, NETWORKS OF ASSOCIATION BETWEEN BACTERIAL TAXA WILL BE ESTABLISHED IN ORDER, AGAIN, TO DERIVE PROPERTIES OF THE COMMUNITIES. THE THIRD SUBPROJECT WILL PAY ATTENTION TO A MODEL GROUP OF MARINE BACTERIA, POLARIBACTER, A GENUS WITHIN THE LARGE GROUP OF BACTEROIDS. THIS GENUS IS RELATIVELY ABUNDANT IN OCEANS, PARTICULARLY IN POLAR WATERS. SOME OF ITS MEMBERS CONTAIN THE PROTEORRODOPSIN GENE, A PROTEIN THAT ALLOWS BACTERIA TO USE LIGHT ENERGY. SP3 WILL ANALYSE THE REGULATION AND RELATIONSHIP OF THIS GROUP OF BACTERIA TO LIGHT. MEMBERS OF THE GENUS WILL BE ISOLATED IN PURE CULTURE AND THEIR GENOMES WILL BE SEQUENCED. AMPLIFIED GENOMES WILL ALSO BE OBTAINED FROM INDIVIDUAL CELLS (SAGS). THESE GENOMES WILL BE USED TO STUDY POLARIBACTER’S CAPABILITIES AND COMPARED TO BACTEROID SEQUENCES DERIVED FROM METAGENOMAS IN SP2. FINALLY, EXPERIMENTS WILL BE CARRIED OUT TO DETERMINE THE GENIC EXPRESSION OF THESE BACTERIA UNDER DIFFERENT DARK LIGHT CONDITIONS AND THE RESULTS WILL BE COMPARED WITH THOSE OBTAINED FROM METATRANSCRIPTOMS IN THE SP2. The OBJECTIVE OF THIS SP3 IS COMPRENDER AS THE CONTROL OF METABOLISM AND THE MANAGEMENT OF METABOLISM IN PRESENCE OF LIGHT IN A HETEROTROFICAL BACTERIA._x000D_ RESULTS have a SIGNIFICATIVE SCIENTIFIC IMPACT IN THE CHANGE OF MICROBIAN Oceanografia and we hope that they open new paths to understand the ecology of the oceans. (English)
12 October 2021
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San Cristóbal de La Laguna
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Identifiers
CTM2013-48292-C3-3-R
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