Reactivation of Human Endogenous Retroviruses (HERV) in Multiple Sclerosis: epigenetic control and diagnostic potential. (Q3153997): Difference between revisions

From EU Knowledge Graph
Jump to navigation Jump to search
(‎Changed label, description and/or aliases in en: translated_label)
(‎Removed claim: summary (P836): Multiple Sclerosis (MS) is a chronic inflammatory and degenerative central nervous system disease (CNS). Since the 1990s, expression of the protein (ENV) of EM-associated Endogenous Retrovirus (HERV-W or MSRV) has been linked to pathology. MSRV is an Endogenous Retrovirus (HERV), which persists in multiple copies in the genome. Apart from MSRV, the expression of at least HERV-K and HERV-M has been associated with MS. Like other Human Endogenou...)
Property / summary
Multiple Sclerosis (MS) is a chronic inflammatory and degenerative central nervous system disease (CNS). Since the 1990s, expression of the protein (ENV) of EM-associated Endogenous Retrovirus (HERV-W or MSRV) has been linked to pathology. MSRV is an Endogenous Retrovirus (HERV), which persists in multiple copies in the genome. Apart from MSRV, the expression of at least HERV-K and HERV-M has been associated with MS. Like other Human Endogenous Retroviruses (HERV), expression is supposed to be controlled by epigenetic mechanisms, especially at the level of DNA methylation. Neither the epigenetic status nor the methylation status of the HERVs have been investigated in the context of MS disease. We suggest investigating the degree of methylation of HERVs (and MSRV in particular) both in PBMC of MS patients and their controls, and in oligodendrocytes/neurons grown in vitro from iPS cells generated by reprogramming of MS patients’ (or controls) cells. In all cases we will also determine MSRV/HERV expression levels in order to relate methylation levels to expression. We will use mass sequencing to determine the whole range of HERV expressed in MS patients. Finally, we will try to generate in vitro models of neuronal cells from patients with MS, to determine the effect of EBV, inflammatory cytokines and the absence of Vitamin D, on the methylation of HERVs and expression levels. (English)
 
Property / summary: Multiple Sclerosis (MS) is a chronic inflammatory and degenerative central nervous system disease (CNS). Since the 1990s, expression of the protein (ENV) of EM-associated Endogenous Retrovirus (HERV-W or MSRV) has been linked to pathology. MSRV is an Endogenous Retrovirus (HERV), which persists in multiple copies in the genome. Apart from MSRV, the expression of at least HERV-K and HERV-M has been associated with MS. Like other Human Endogenous Retroviruses (HERV), expression is supposed to be controlled by epigenetic mechanisms, especially at the level of DNA methylation. Neither the epigenetic status nor the methylation status of the HERVs have been investigated in the context of MS disease. We suggest investigating the degree of methylation of HERVs (and MSRV in particular) both in PBMC of MS patients and their controls, and in oligodendrocytes/neurons grown in vitro from iPS cells generated by reprogramming of MS patients’ (or controls) cells. In all cases we will also determine MSRV/HERV expression levels in order to relate methylation levels to expression. We will use mass sequencing to determine the whole range of HERV expressed in MS patients. Finally, we will try to generate in vitro models of neuronal cells from patients with MS, to determine the effect of EBV, inflammatory cytokines and the absence of Vitamin D, on the methylation of HERVs and expression levels. (English) / rank
Normal rank
 
Property / summary: Multiple Sclerosis (MS) is a chronic inflammatory and degenerative central nervous system disease (CNS). Since the 1990s, expression of the protein (ENV) of EM-associated Endogenous Retrovirus (HERV-W or MSRV) has been linked to pathology. MSRV is an Endogenous Retrovirus (HERV), which persists in multiple copies in the genome. Apart from MSRV, the expression of at least HERV-K and HERV-M has been associated with MS. Like other Human Endogenous Retroviruses (HERV), expression is supposed to be controlled by epigenetic mechanisms, especially at the level of DNA methylation. Neither the epigenetic status nor the methylation status of the HERVs have been investigated in the context of MS disease. We suggest investigating the degree of methylation of HERVs (and MSRV in particular) both in PBMC of MS patients and their controls, and in oligodendrocytes/neurons grown in vitro from iPS cells generated by reprogramming of MS patients’ (or controls) cells. In all cases we will also determine MSRV/HERV expression levels in order to relate methylation levels to expression. We will use mass sequencing to determine the whole range of HERV expressed in MS patients. Finally, we will try to generate in vitro models of neuronal cells from patients with MS, to determine the effect of EBV, inflammatory cytokines and the absence of Vitamin D, on the methylation of HERVs and expression levels. (English) / qualifier
point in time: 12 October 2021
Timestamp+2021-10-12T00:00:00Z
Timezone+00:00
CalendarGregorian
Precision1 day
Before0
After0
 

Revision as of 15:46, 12 October 2021

Project Q3153997 in Spain
Language Label Description Also known as
English
Reactivation of Human Endogenous Retroviruses (HERV) in Multiple Sclerosis: epigenetic control and diagnostic potential.
Project Q3153997 in Spain

    Statements

    country
    Spain
    0 references
    EU contribution
    33,000.0 Euro
    0 references
    budget
    66,000.0 Euro
    0 references
    co-financing rate
    50.0 percent
    0 references
    start time
    1 January 2014
    0 references
    end time
    30 March 2018
    0 references
    beneficiary name (string)
    INSTITUTO ARAGONES DE CIENCIAS DE LA SALUD
    0 references
    coordinate location

    41°39'7.67"N, 0°52'51.38"W
    0 references
    postal code
    50297
    0 references
    summary
    La Esclerosis múltiple (EM) es una enfermedad crónica inflamatoria y degenerativa del sistema nervioso central (CNS). Ya desde los años 90 se ha relacionado la expresión de de la proteína (ENV) del Retrovirus Endógeno asociado a EM (HERV-W o MSRV) con la patología. MSRV es un Retrovirus Endógeno (HERV), que persiste en multiple copias en el genóma. Aparte de MSRV, la expresión de por lo menos HERV-K y HERV-M ha sido asociado a EM. Como otros Retrovirus Endógenos Humanos (HERV), la expresión se supone controlado por mecanismos epigenéticos, especialmente al nivel de metilación del ADN. Ni el estado epigenético, ni el estado de la metilación de los HERV han sido investigado en el contexto de la enfermedad EM. Sugerimos investigar el grado de metilación de los HERVs (y de MSRV en particular) tanto en PBMC de pacientes de EM y sus controles, como en oligodendrocitos/neuronas cultivados in vitro a partir de células iPS generadas por reprogramación de células de pacientes con EM (o controles). En todos los casos determinaremos también los niveles de expresión de MSRV/HERV con el objetivo de relacionar niveles de metilación con la expresión. Utilizaremos secuenciación masiva con el fin de determinar el abanico entero de HERV expresados en pacientes con EM. Como último, intentaremos generar modelos in vitro de células neuronales a partir de pacientes con EM, para determinar el efecto de EBV, de citokinas inflamatorias y de la ausencia de Vitamina D, sobre la metilación de los HERV y los niveles de expresión. (Spanish)
    0 references
    location (string)
    Zaragoza
    0 references

    Identifiers

    Spanish Kohesio ID
    PI13_02518
    0 references
     
    Retrieved from "https://linkedopendata.eu/w/index.php?title=Item:Q3153997&oldid=12815538"