TRANSMISSION SOURCES OF ESCHERICHIA COLI PANDEMIC CLONAL GROUP ST131. MOLECULAR CHARACTERISATION OF THE PATHOGEN AND ITS VIRULENCE IN VIVO, RISK ASSESSMENT AND CONTROL (Q3136046)
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Project Q3136046 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | TRANSMISSION SOURCES OF ESCHERICHIA COLI PANDEMIC CLONAL GROUP ST131. MOLECULAR CHARACTERISATION OF THE PATHOGEN AND ITS VIRULENCE IN VIVO, RISK ASSESSMENT AND CONTROL |
Project Q3136046 in Spain |
Statements
91,960.0 Euro
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114,950.0 Euro
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80.0 percent
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1 January 2014
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30 June 2017
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UNIVERSIDAD DE SANTIAGO DE COMPOSTELA
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43°2'46.39"N, 7°28'26.36"W
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27028
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EL ESTUDIO DE INVESTIGACION PROPUESTO EN LA PRESENTE SOLICITUD SE ENMARCA DENTRO DEL RETO "SEGURIDAD Y CALIDAD ALIMENTARIA". EL GRUPO CLONAL DE E. COLI ST131 ROMPE EL PARADIGMA DE LA INCOMPATIBILIDAD DE LOS GENES DE RESISTENCIA A ANTIBIOTICOS Y LOS DE VIRULENCIA EN LOS DE E. COLI PATOGENOS EXTRAINTESTINALES (EXPEC). DESDE SU IDENTIFICACION A NIVEL INTERCONTINENTAL, EN EL AÑO 2008, ESTE GRUPO CLONAL HA TENIDO UNA EXPANSION ESPECTACULAR, REPRESENTANDO EN LA ACTUALIDAD MAS DEL 50% DE LAS CEPAS PRODUCTORAS DE BETA LACTAMASAS DE ESPECTRO EXTENDIDO (ESBL) Y MAS DEL 30% DE LAS CEPAS DE EXPEC MULTIRRESISTENTES. EN EL PRESENTE PROYECTO QUEREMOS DEFINIR CLARAMENTE CUALES SON LAS RUTAS Y FUENTES DE TRANSMISION DEL GRUPO CLONAL ST131, SI EXISTE RIESGO, Y QUE GRADO DE RIESGO EN EL CONSUMO DE CARNE DE ANIMALES DE PRODUCCION, Y QUE MEDIDAS DE CONTROL SE PUEDEN APLICAR EN LA CADENA ALIMENTARIA. QUEREMOS ANALIZAR TAMBIEN EL PAPEL DE LA FAUNA SILVESTRE EN EL MANTENIMIENTO Y DISEMINACION DE CEPAS ST131. CONCRETAMENTE ESTUDIAREMOS AVES SILVESTRES, ZORROS Y JABALIES, QUE POR SU EXPLOSION DEMOGRAFICA MANTIENEN ACTUALMENTE UN IMPORTANTE CONTACTO CON ANIMALES DE PRODUCCION Y ASENTAMIENTOS HUMANOS. SEGUN NUESTROS DATOS ES LA PRIMERA VEZ SE INVESTIGAN LOS ZORROS Y LOS JABALIES COMO PORTADORES DE CEPAS ST131, Y AUNQUE SI SE HAN AISLADO CEPAS ST131 DE AVES SILVESTRES EN VARIOS PAISES, NO SE HAN ESTABLECIDO SUS VIROTIPOS NI SE HAN COMPARADO CON LAS CEPAS RESPONSABLES DE INFECCIONES EN SERES HUMANOS. VAMOS A REALIZAR UNA DETALLADA CARACTERIZACION MOLECULAR (MLST, PFGE, SECUENCIACION COMPLETA DEL GENOMA PARA DEFINIR GENES DE VIRULENCIA E ISLAS DE PATOGENICIDAD) DE LAS CEPAS ST131 AISLADAS DE ALIMENTOS Y ANIMALES, Y DETERMINAREMOS SU VIRULENCIA IN VIVO EN UN MODELO DE SEPSIS MURINO. COMPARAREMOS LOS DATOS OBTENIDOS CON LOS DE LAS CEPAS ST131 CAUSANTES DE PATOLOGIA EN SERES HUMANOS. EL OBJETIVO ES QUE LOS RESULTADOS REPERCUTAN EN LA SALUD PUBLICA Y LA SEGURIDAD ALIMENTARIA A TRAVES DE UN MAYOR CONOCIMIENTO DE LOS MECANISMOS QUE HACEN EXITOSO A ESTE GRUPO CLONAL DESDE EL PUNTO DE VISTA DE LA VIRULENCIA Y LA DISEMINACION. EL IMPACTO CIENTIFICO DE LOS RESULTADOS ESTA TOTALMENTE ASEGURADO Y ACREDITADO POR EL ELEVADO INDICE DE IMPACTO DE LAS PUBLICACIONES QUE VENIMOS REALIZADO DESDE QUE EN EL AÑO 2008 FORMASEMOS PARTE DEL CONSORCIO INTERNACIONAL QUE EVIDENCIO LA DISTRIBUCION INTERCONTINENTAL DEL GRUPO CLONAL ST131. (Spanish)
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THE RESEARCH STUDY PROPOSED IN THIS APPLICATION FALLS WITHIN THE “FOOD SAFETY AND QUALITY” CHALLENGE. THE CLONAL GROUP OF E. COLI ST131 BREAKS THE PARADIGM OF THE INCOMPATIBILITY OF ANTIBIOTIC RESISTANCE GENES AND THOSE OF VIRULENCE IN THOSE OF E. COLI EXTRAINTESTINAL PATHOGENS (EXPEC). SINCE ITS IDENTIFICATION AT THE INTERCONTINENTAL LEVEL, IN 2008, THIS CLONAL GROUP HAS HAD A SPECTACULAR EXPANSION, CURRENTLY ACCOUNTING FOR MORE THAN 50 % OF THE BETA-PRODUCING STRAINS OF EXTENDED SPECTRUM LACTAMASES (ESBL) AND MORE THAN 30 % OF MULTIDRUG RESISTANT EXPEC STRAINS. IN THIS PROJECT WE WANT TO CLEARLY DEFINE THE ROUTES AND SOURCES OF TRANSMISSION OF THE CLONAL GROUP ST131, IF THERE IS A RISK, AND WHAT DEGREE OF RISK IN THE CONSUMPTION OF MEAT FROM PRODUCTION ANIMALS, AND WHICH CONTROL MEASURES CAN BE APPLIED IN THE FOOD CHAIN. WE ALSO WANT TO ANALYSE THE ROLE OF WILDLIFE IN THE MAINTENANCE AND DISSEMINATION OF ST131 STRAINS. SPECIFICALLY WE WILL STUDY WILD BIRDS, FOXES AND JABALIES, WHICH DUE TO THEIR DEMOGRAPHIC EXPLOSION CURRENTLY MAINTAIN IMPORTANT CONTACT WITH ANIMALS OF PRODUCTION AND HUMAN SETTLEMENTS. ACCORDING TO OUR DATA THIS IS THE FIRST TIME FOXES AND JABALIES ARE INVESTIGATED AS CARRIERS OF STRAINS ST131, AND ALTHOUGH IF ST131 STRAINS OF WILD BIRDS HAVE BEEN ISOLATED IN SEVERAL COUNTRIES, THEIR VIROTIPOS HAVE NOT BEEN ESTABLISHED NOR COMPARED TO THE STRAINS RESPONSIBLE FOR INFECTIONS IN HUMANS. WE WILL PERFORM A DETAILED MOLECULAR CHARACTERISATION (MLST, PFGE, COMPLETE GENOME SEQUENCING TO DEFINE VIRULENCE GENES AND ISLANDS OF PATHOGENICITY) OF ST131 STRAINS ISOLATED FROM FOOD AND ANIMALS, AND WE WILL DETERMINE THEIR VIRULENCE IN VIVO IN A MODEL OF MURINE SEPSIS. WE WILL COMPARE THE DATA OBTAINED WITH THOSE OF THE ST131 STRAINS CAUSING PATHOLOGY IN HUMANS. THE OBJECTIVE IS THAT THE RESULTS HAVE AN IMPACT ON PUBLIC HEALTH AND FOOD SAFETY THROUGH GREATER KNOWLEDGE OF THE MECHANISMS THAT MAKE THIS CLONAL GROUP SUCCESSFUL FROM THE POINT OF VIEW OF VIRULENCE AND DISSEMINATION. THE SCIENTIFIC IMPACT OF THE RESULTS IS FULLY ASSURED AND ACCREDITED BY THE HIGH IMPACT INDEX OF THE PUBLICATIONS THAT WE HAVE PRODUCED SINCE 2008 FORMASEMOS PART OF THE INTERNATIONAL CONSORTIUM THAT DEMONSTRATED THE INTERCONTINENTAL DISTRIBUTION OF THE CLONAL GROUP ST131. (English)
12 October 2021
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Lugo
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Identifiers
AGL2013-47852-R
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