INNOVATIVE TECHNOLOGIES FOR THE PRODUCTION OF BIOLOGICAL INSECTICIDES WITH GREATER EFFICIENCY AND SPECTRUM OF GUESTS (Q3135674)
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Project Q3135674 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | INNOVATIVE TECHNOLOGIES FOR THE PRODUCTION OF BIOLOGICAL INSECTICIDES WITH GREATER EFFICIENCY AND SPECTRUM OF GUESTS |
Project Q3135674 in Spain |
Statements
175,450.0 Euro
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350,900.0 Euro
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50.0 percent
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1 January 2015
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31 May 2018
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UNIVERSIDAD PUBLICA DE NAVARRA
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42°49'6.42"N, 1°38'39.34"W
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31201
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EL DESARROLLO DE EFECTIVOS AGENTES DE CONTROL BIOLOGICO CONTRA PLAGAS PUEDE REDUCIR NOTABLEMENTE O ELIMINAR LAS NECESIDADES DE PLAGUICIDAS QUIMICOS. POR TANTO, DICHOS AGENTES DE CONTROL DE PLAGAS SON UN OBJETIVO PRIORITARIO PARA PODER DESARROLLAR UNA ACTIVIDAD AGRARIA SOSTENIBLE ASI COMO PARA MEJORAR LA SEGURIDAD Y CALIDAD AGROALIMENTARIAS. LOS BIOINSECTICIDAS BASADOS EN BACULOVIRUS SON UN MEDIO SEGURO Y EFECTIVO PARA EL CONTROL DE MUCHAS DE PLAGAS DE LEPIDOPTEROS DE GRAN IMPORTANCIA ECONOMICA. SIN EMBARGO, LA EXCESIVA ESPECIFICIDAD DE LOS BACULOVIRUS HA LIMITADO SU COMERCIALIZACION Y UTILIZACION POR LOS AGRICULTORES. LOS INTENTOS PREVIOS DE AMPLIAR EL ESPECTRO INSECTICIDA DE ESTOS VIRUS HAN SIDO FALLIDOS. RECIENTEMENTE, HEMOS DEMOSTRADO QUE TRAS LA INFECCION DE UNA CELULA DE INSECTO POR UN BACULOVIRUS, EXISTE UNA VENTANA TEMPORAL DURANTE LA CUAL UNA SEGUNDA ESPECIE DE VIRUS PUEDE INFECTAR DICHA CELULA. APROVECHANDO LA ESTRUCTURA FISICA DE ESTOS VIRUS, HEMOS PRODUCIDO VIRIONES (ODVS) QUE CONTIENEN MEZCLAS DE DIFERENTES ESPECIES DE VIRUS. ESTOS ODVS QUEDAN OCLUIDOS EN UNA MISMA MATRIZ PROTEICA FORMANDO OBS MIXTOS QUE CONTIENEN COMBINACIONES DE LOS VIRUS PARENTALES. LA PROPORCION DE CADA VIRUS EN LOS OBS PUEDE SER CONTROLADA AJUSTANDO LAS CARACTERISTICAS DEL INOCULO Y LA SECUENCIA TEMPORAL DE INOCULACION. ESTA TECNOLOGIA DE CO-OCLUSION DE BACULOVIRUS LA HEMOS VALIDADO TANTO PARA ESPECIES DE BACULOVIRUS CERCANAS COMO DISTANTES (PATENTE EUROPEA PCT/EP2013/069678) POR LO QUE, ADICIONALMENTE, PROPORCIONA UNA HERRAMIENTA UNICA PARA IDENTIFICAR LOS FACTORES MOLECULARES QUE REGULAN EL ESPECTRO DE HUESPEDES EN LOS BACULOVIRUS. EN OTROS ESTUDIOS HEMOS IDENTIFICADO MOLECULAS NATURALES QUE INTERFIEREN CON EL SISTEMA INMUNE DE LOS INSECTOS. POR EJ., CIERTOS METABOLITOS SECUNDARIOS DE PLANTAS INHIBEN LA MELANIZACION Y DETERMINADOS AZUCARES PUEDEN AUMENTAR LA POTENCIA DE LOS BACULOVIRUS AL BLOQUEAR DE FORMA ESPECIFICA ALGUNAS LECTINAS INTESTINALES QUE INTERFIEREN CON LA CAPACIDAD INFECTIVA DEL VIRUS. POR TANTO, NUESTRA HIPOTESIS DE TRABAJO ES QUE LA TECNOLOGIA DE LA CO-OCLUSION DE BACULOVIRUS REPRESENTA UNA ALTERNATIVA NO RECOMBINANTE QUE PERMITIRA OBTENER NUEVOS BIOINSECTICIDAS BASADOS EN BACULOVIRUS, CON MAYOR ESPECTRO DE HUESPEDES Y MEJORES PROPIEDADES INSECTICIDAS. ESTOS NUEVOS BACULOVIRUS, SOLOS O EN COMBINACION CON METABOLITOS ADYUVANTES, PUEDEN SER MUCHO MAS EFECTIVOS PARA COMBATIR COMPLEJOS DE ESPECIES EN LOS PRINCIPALES CULTIVOS DE CAMPO E INVERNADERO. ESTO SE CONSEGUIRA A TRAVES DE UNA CUIDADA SELECCION DE COMBINACIONES DE GENOTIPOS ESPECIFICOS DE DIFERENTES ESPECIES DE VIRUS Y LA IDENTIFICACION DE LAS CELULAS O LARVAS DE INSECTOS DONDE DICHOS VIRUS PUEDAN REPLICARSE SIMULTANEAMENTE PARA GENERAR OBS Y ODVS MIXTOS CON LAS DESEADAS PROPIEDADES INSECTICIDAS Y MEDIANTE EL ESTUDIO Y DESCUBRIMIENTO DE NUEVOS ELEMENTOS A INCLUIR EN LA FORMULACION QUE POTENCIEN LA ACCION DE BACULOVIRUS. ESTOS OBS SERAN EVALUADOS EN CONDICIONES DE CAMPO PARA DETERMINAR SU EFICACIA A DIFERENTES CONCENTRACIONES EN ALGUNOS CULTIVOS IMPORTANTES (SOJA, TOMATE, FRESA) QUE SON DAÑADOS POR COMPLEJOS DE ESPECIES DE LEPIDOPTEROS DEFOLIADORES. DADA LA ALTA PROBABILIDAD DE QUE ESTE PROYECTO GENERE INNOVACIONES APLICABLES AL DESARROLLO COMERCIAL DE NUEVOS BIOINSECTICIDAS BASADOS EN ESTA TECNOLOGIA, ALGUNOS DE ESTOS RESULTADOS DEBERAN SER TRATADOS CONFIDENCIALMENTE HASTA QUE LOS DERECHOS DE PROPIEDAD INTELECTUAL HAYAN SIDO ADECUADAMENTE PROTEGIDOS. (Spanish)
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THE DEVELOPMENT OF EFFECTIVE BIOLOGICAL PEST CONTROL AGENTS CAN SIGNIFICANTLY REDUCE OR ELIMINATE THE NEEDS FOR CHEMICAL PESTICIDES. SUCH PEST CONTROL AGENTS ARE THEREFORE A PRIORITY OBJECTIVE IN ORDER TO BE ABLE TO DEVELOP A SUSTAINABLE AGRICULTURAL ACTIVITY AS WELL AS TO IMPROVE AGRI-FOOD SAFETY AND QUALITY. BACULOVIRUS-BASED BIOINSECTICIDES ARE A SAFE AND EFFECTIVE MEANS OF CONTROLLING MANY OF LEPIDOPTERAN PESTS OF GREAT ECONOMIC IMPORTANCE. HOWEVER, THE EXCESSIVE SPECIFICITY OF BACULOVIRUSES HAS LIMITED THEIR MARKETING AND USE BY FARMERS. PREVIOUS ATTEMPTS TO EXPAND THE INSECTICIDE SPECTRUM OF THESE VIRUSES HAVE BEEN FAILED. RECENTLY, WE HAVE SHOWN THAT AFTER INFECTION OF AN INSECT CELL BY A BACULOVIRUS, THERE IS A TEMPORARY WINDOW DURING WHICH A SECOND SPECIES OF VIRUS CAN INFECT THE CELL. TAKING ADVANTAGE OF THE PHYSICAL STRUCTURE OF THESE VIRUSES, WE HAVE PRODUCED VIRIONS (ODVS) THAT CONTAIN MIXTURES OF DIFFERENT SPECIES OF VIRUSES. THESE ODVS ARE OCCLUDED IN THE SAME PROTEIN MATRIX FORMING MIXED OBSS CONTAINING COMBINATIONS OF PARENTAL VIRUSES. THE PROPORTION OF EACH VIRUS IN THE OBS CAN BE CONTROLLED BY ADJUSTING THE CHARACTERISTICS OF THE INOCULUM AND THE TEMPORAL SEQUENCE OF INOCULATION. THIS BACULOVIRUS CO-OCLUSION TECHNOLOGY HAS BEEN VALIDATED FOR BOTH NEARBY AND DISTANT BACULOVIRUS SPECIES (EUROPEAN PATENT PCT/EP2013/069678) SO IT ADDITIONALLY PROVIDES A UNIQUE TOOL TO IDENTIFY THE MOLECULAR FACTORS THAT REGULATE THE SPECTRUM OF GUESTS IN BACULOVIRUSES. IN OTHER STUDIES WE HAVE IDENTIFIED NATURAL MOLECULES THAT INTERFERE WITH THE IMMUNE SYSTEM OF INSECTS. FOR EXAMPLE, CERTAIN SECONDARY PLANT METABOLITES INHIBIT MELANISATION AND CERTAIN SUGARS MAY INCREASE THE POTENCY OF BACULOVIRUSES BY SPECIFICALLY BLOCKING SOME INTESTINAL LECTINS THAT INTERFERE WITH THE VIRUS’S INFECTIVE CAPACITY. THEREFORE, OUR WORKING HYPOTHESIS IS THAT THE CO-OCLUSION TECHNOLOGY OF BACULOVIRUS REPRESENTS A NON-RECOMBINANT ALTERNATIVE THAT WOULD ALLOW TO OBTAIN NEW BIOINSECTICIDES BASED ON BACULOVIRUS, WITH A GREATER SPECTRUM OF GUESTS AND BETTER INSECTICIDE PROPERTIES. THESE NEW BACULOVIRUSES, ALONE OR IN COMBINATION WITH ADJUVANT METABOLITES, CAN BE MUCH MORE EFFECTIVE IN FIGHTING SPECIES COMPLEXES IN THE MAIN FIELD AND GREENHOUSE CROPS. THIS WILL BE ACHIEVED THROUGH A CAREFUL SELECTION OF COMBINATIONS OF SPECIFIC GENOTYPES OF DIFFERENT SPECIES OF VIRUSES AND THE IDENTIFICATION OF INSECT CELLS OR LARVAE WHERE THESE VIRUSES CAN BE REPLICATED SIMULTANEOUSLY TO GENERATE MIXED OBS AND ODVS WITH THE DESIRED INSECTICIDE PROPERTIES AND BY STUDYING AND DISCOVERING NEW ELEMENTS TO BE INCLUDED IN THE FORMULATION THAT ENHANCE THE ACTION OF BACULOVIRUS. THESE OBS WILL BE EVALUATED UNDER FIELD CONDITIONS TO DETERMINE THEIR EFFICACY AT DIFFERENT CONCENTRATIONS IN SOME IMPORTANT CULTURES (SOYBEAN, TOMATO, STRAWBERRY) THAT ARE DAMAGED BY COMPLEXES OF DEFOLIATING LEPIDOPTERA SPECIES. GIVEN THE HIGH PROBABILITY THAT THIS PROJECT WILL GENERATE INNOVATIONS APPLICABLE TO THE COMMERCIAL DEVELOPMENT OF NEW BIOINSECTICIDES BASED ON THIS TECHNOLOGY, SOME OF THESE RESULTS SHOULD BE TREATED CONFIDENTIALLY UNTIL INTELLECTUAL PROPERTY RIGHTS HAVE BEEN ADEQUATELY PROTECTED. (English)
12 October 2021
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Pamplona/Iruña
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Identifiers
AGL2014-57752-C2-1-R
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