Identification of chromatin factors affecting meiotic crossover formation in plants (Q84329)
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Project Q84329 in Poland
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | Identification of chromatin factors affecting meiotic crossover formation in plants |
Project Q84329 in Poland |
Statements
3,499,750.0 zloty
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3,499,750.0 zloty
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100.0 percent
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1 December 2018
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30 November 2021
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UNIWERSYTET IM. ADAMA MICKIEWICZA W POZNANIU
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Q2513981 (Deleted Item)
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Meiotic crossover is a fundamental process that unlocks and generates genetic diversity and is therefore an important substrate for crop breeding. In this proposal, we seek to characterize the chromatin factors controlling meiotic crossover in plant genomes. First, we will develop a new approach which will allow us to study selected crossover hotspots in Arabidopsis with high resolution. This will be based on recombinant seed sorting with fluorescent reporters and successive sequencing of crossover-containing intervals. Next, we will directly test effects of two types of chromatin modification on crossover distribution: histone H3 trimethylation at lysine 4 (H3K4me3), and histone H4 acetylation. For this purpose, we will target histone modifying enzymes to crossover hotspots within our intervals by CRISPR/dCas9 system. This project will lead to new discoveries on the crossover control and will pave a way to generate artificial recombination hotspots for modern plant breeding. (Polish)
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Meiotic crossover is a fundamental process that unlocks and generates genetic diversity and is therefore an important substrate for crop breeding. In this proposal, we seek to characterise the chromatin factors controlling meiotic crossover in plant genomes. First, we will develop a new approach which will allow us to study selected crossover hotspots in Arabidopsis with high resolution. This will be based on recombinant seed sorting with fluorescent Reporters and successive sequencing of crossover-containing intervals. Next, we will directly test effects of two types of chromatin modification on crossover distribution: histone H3 trimethylation at lysine 4 (H3K4me3), and histone H4 acetylation. For this purpose, we will target histone modifying enzymes to crossover hotspots within our intervals by CRISPR/dCas9 system. This project will lead to new discoveries on the crossover control and will pave a way to generate artificial recombination hotspots for modern plant breeding. (English)
14 October 2020
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Le croisement méiotique est un processus fondamental qui débloque et génère la diversité génétique et constitue donc un substrat important pour la sélection des cultures. Dans cette proposition, nous cherchons à caractériser les facteurs de chromatine contrôlant le croisement méiotique dans les génomes végétaux. Tout d’abord, nous allons développer une nouvelle approche qui nous permettra d’étudier des points chauds de croisement sélectionnés dans Arabidopsis à haute résolution. Ceci sera basé sur le tri des graines recombinantes avec des reporters fluorescents et le séquençage successif des intervalles de croisement. Ensuite, nous testerons directement les effets de deux types de modification de la chromatine sur la distribution croisée: triméthylation de l’histone H3 à la lysine 4 (H3K4me3) et acétylation de l’histone H4. À cette fin, nous allons cibler les enzymes de modification de l’histone vers des points chauds croisés à l’intérieur de nos intervalles par le système CRISPR/dCas9. Ce projet mènera à de nouvelles découvertes sur le contrôle croisé et ouvrira la voie à la recombinaison artificielle de points chauds pour la sélection végétale moderne. (French)
30 November 2021
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Identifiers
POIR.04.04.00-00-5C0F/17
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