REVALUATION OF AGRICULTURAL BY-PRODUCTS THROUGH THE USE OF GENETICALLY MODIFIED MICROBIAL BIOTECHNOLOGICAL PLATFORMS FOR THE PRODUCTION OF TERPENES AND TERPENOIDS (Q3147623)
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Project Q3147623 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | REVALUATION OF AGRICULTURAL BY-PRODUCTS THROUGH THE USE OF GENETICALLY MODIFIED MICROBIAL BIOTECHNOLOGICAL PLATFORMS FOR THE PRODUCTION OF TERPENES AND TERPENOIDS |
Project Q3147623 in Spain |
Statements
35,574.0 Euro
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71,148.0 Euro
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50.0 percent
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1 January 2019
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31 December 2021
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ASOCIACION DE INVESTIGACION DEL INSTITUTO DE BIOTECNOLOGIA DE LEON (INBIOTEC)
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42°38'2.94"N, 5°58'17.11"W
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24089
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LA BIOMASA SE POSICIONA COMO UNA FUENTE RENOVABLE DE ENERGIA, PERO TAMBIEN DE CARBONO, CON LA QUE ES POSIBLE PRODUCIR UN AMPLIO ABANICO DE PRODUCTOS DE ALTO VALOR AÑADIDO A TRAVES DE UN PROCESO DE BIORREFINERIA. DE ENTRE LOS COMPUESTOS QUIMICOS CUYO INTERES ESTA CRECIENDO SE ENCUENTRAN LOS TERPENOIDES, METABOLITOS SECUNDARIOS CON GRAN APLICACION INDUSTRIAL COMO AROMAS EN ALIMENTACION, COSMETICA Y FARMACIA, ADEMAS DE QUE ALGUNOS DE ELLOS POSEEN IMPORTANTES ACTIVIDADES BIOLOGICAS. UNO DE LOS TERPENOIDES MAS IMPORTANTES ES EL LIMONENO, QUE ADEMAS SIRVE COMO PRECURSOR PARA LA PRODUCCION DE OTROS MUCHOS DERIVADOS DE INTERES INDUSTRIAL. EL LIMONENO ADEMAS DE TENER GRAN USO COMO AROMA EN ALIMENTACION (SABOR A LIMON) POSEE ACTIVIDAD ANTICANCERIGENA, ANTIINFLAMATORIA E INMUNOMODULADORA. A PARTIR DEL LIMONENO SE PUEDEN PRODUCIR OTROS TERPENOIDES COMO EL LIMONENO 1,2 EPOXIDO QUE TAMBIEN ES AMPLIAMENTE UTILIZADO COMO AROMA Y FRAGANCIA Y PODRIA TENER APLICACIONES FARMACOLOGICAS DEBIDO A SU EFECTO ANSIOLITICO EN RATON. OTROS TERPENOIDES IMPORTANTES SON LAS XANTOFILAS ZEAXANTINA Y ASTAXANTINA. LA ZEAXANTINA ES UN ALCOHOL DE COLOR AMARILLO IMPORTANTE PARA LA VISION, YA QUE EJERCEN UNA ACCION PROTECTORA FRENTE A DIVERSAS ENFERMEDADES OCULARES. POR SU PARTE, LA ASTAXANTINA ES UN PIGMENTO ROJO-ANARANJADO CON FUERTES PROPIEDADES ANTIOXIDANTES Y SE EMPLEA SE USA COMO SUPLEMENTO EN PIENSOS._x000D_ LA CRECIENTE DEMANDA POR PRODUCTOS NATURALES HACE QUE HAYA AUMENTADO CONSIDERABLEMENTE EL INTERES POR LA PRODUCCION DE ESTOS COMPUESTOS MEDIANTE PROCESOS FERMENTATIVOS, PARA LO QUE NORMALMENTE SE REQUIERE DE PROCESOS DE INGENIERIA GENETICA PARA CONSEGUIR RENDIMIENTOS ADECUADOS CON FINES INDUSTRIALES. EL OBJETIVO DE ESTE SUBPROYECTO ES LA REVALORIZACION DE DESECHOS AGRICOLAS (PAJA DE MAIZ Y PULPA DE REMOLACHA) PROVENIENTES DE LA ZONA GEOGRAFICA CON MAYOR PRODUCCION DE ESTOS PRODUCTOS (LEON Y ZONAS LIMITROFES) MEDIANTE SU BIOTRANSFORMACION EN TERPENOIDES NATURALES DE INTERES. PARA ELLO SE PLANTEA EL USO DE PLATAFORMAS BIOTECNOLOGICAS CONSISTENTES EN MICROORGANISMOS MODIFICADOS MEDIANTE INGENIERIA GENETICA. POR UNA PARTE LA BACTERIA STREPTOMYCES LIVIDANS SE ADAPTARA PARA LA PRODUCCION DE LIMONENO Y LIMONENO 1,2 EPOXIDO MEDIANTE LA SOBREEXPRESION SECUENCIAL DEL GEN CODIFICANTE DE LA ENZIMA 4S-LIMONENO_x000D_ SINTASA DE MENTHA SPICATA Y DEL GEN QUE CODIFICA LA ENZIMA LIMONENO 1,2 MONOOXIGENASA (LIMB) DE RHODOCOCCUS ERYTHROPOLIS DCL14. EL HONGO LEVADURIFORME XANTHOPHYLLOMYCES DENDRORHOUS SE MODIFICARA PARA MEJORAR LA PRODUCCION DE ASTAXANTINA MEDIANTE LA SOBREEXPRESION ORTOLOGA DE LOS GENES QUE CODIFICAN LA FITOENO SINTASA (GEN CRTYB) Y LA ASTAXANTINA SINTASA (CRTS). POR OTRO LADO, PARA OBTENER MUTANTES SOBREPRODUCTORES DE ZEAXANTINA, SE PARTIRA DE UNA CEPA MUTANTE DE X. DENDRORHOUS QUE ACUMULA EL PRECURSOR BCAROTENO, Y SOBRE LA QUE SE SOBREEXPRESARAN DIVERSOS GENES ORTOLOGOS DE LA RUTA BIOSINTETICA Y LA B-CAROTENO C3-HIDROXILASA (CODIFICADA POR EL GEN CRTZ) DERIVADA DE BREVUNDIMONAS SP. SD212. UNA VEZ OBTENIDAS LAS PLATAFORMAS BIOTECNOLOGICAS, SE PLANTEA LA OPTIMIZACION DE LA PRODUCCION DE ESTOS COMPUESTOS PARA PROCEDER A SU POSTERIOR RECUPERACION Y PURIFICACION. ESTOS TERPENOIDES NATURALES CONSTITUIRAN LAS UNIDADES BASE (BUILDING BLOCKS) PARA SU POSTERIOR POLIMERIZACION POR EL EQUIPO DE INVESTIGACION DEL SUBPROYECTO 1. (Spanish)
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BIOMASS, APART FROM BEING JUST A RENEWABLE SOURCE OF ENERGY, REPRESENTS AN IMPORTANT CARBON SOURCE FOR THE PRODUCTION OF A WIDE RANGE OF PRODUCTS WITH HIGH ADDED VALUE THROUGH A PROCESS OF BIOREFINERY. AMONG THOSE CHEMICAL COMPOUNDS HAVING A GROWING INTEREST, TERPENOIDS ARE SECONDARY METABOLITES WITH GREAT INDUSTRIAL APPLICATION AS AROMAS IN FOOD, COSMETICS AND PHARMACY, AND SOME OF THEM HAVE IMPORTANT BIOLOGICAL ACTIVITIES. ONE OF THE MOST IMPORTANT TERPENOIDS IS LIMONENE, WHICH ALSO SERVES AS A PRECURSOR FOR THE PRODUCTION OF MANY OTHER_x000D_ DERIVATIVES OF INDUSTRIAL INTEREST. LIMONENE, IN ADDITION TO ITS BROAD USE AS AN AROMA IN FOOD (LEMON FLAVOR), HAS ANTICANCER, ANTIINFLAMMATORY AND IMMUNOMODULATORY ACTIVITY. OTHER TERPENOIDS, SUCH AS LIMONENE 1,2 EPOXIDE, CAN BE PRODUCED FROM LIMONENE, WHICH IS ALSO WIDELY USED AS AROMA AND FRAGRANCE AND COULD HAVE PHARMACOLOGICAL APPLICATIONS DUE TO ITS ANXIOLYTIC EFFECT IN MICE. OTHER IMPORTANT TERPENOIDS ARE THE XANTHOPHYLLS ZEAXANTHIN AND ASTAXANTHIN. ZEAXANTHIN IS A YELLOW ALCOHOL IMPORTANT FOR VISION, AS IT EXERTS A PROTECTIVE ACTION AGAINST VARIOUS EYE DISEASES. FOR ITS PART, ASTAXANTHIN IS A RED-ORANGE PIGMENT WITH STRONG ANTIOXIDANT PROPERTIES AND IS USED AS A SUPPLEMENT IN FEED._x000D_ THE GROWING DEMAND FOR NATURAL PRODUCTS HAS CONSIDERABLY INCREASED THE INTEREST IN THE PRODUCTION OF THESE COMPOUNDS THROUGH FERMENTATION PROCESSES, WHICH USUALLY REQUIRES GENETIC ENGINEERING PROCESSES TO ACHIEVE ADEQUATE YIELDS FOR INDUSTRIAL PURPOSES. THE OBJECTIVE OF THIS SUBPROJECT IS THE VALORIZATION OF AGRICULTURAL WASTES (MAIZE STRAW AND BEET PULP) OBTAINED FROM THE LEADING MAIZE AND BEET PRODUCER GEOGRAPHIC AREA (LEON AND NEIGHBORING AREAS) BY MEANS OF THEIR BIOTRANSFORMATION IN NATURAL TERPENOIDS OF INTEREST. TO THIS END, THE USE OF BIOTECHNOLOGICAL PLATFORMS CONSISTING OF GENETICALLY ENGINEERED MICROORGANISMS IS PROPOSED. THE BACTERIA STREPTOMYCES LIVIDANS WILL BE MODIFIED FOR THE PRODUCTION OF LIMONENE AND LIMONENE 1,2 EPOXIDE BY MEANS OF PROGRESSIVE OVEREXPRESSION OF THE GENE ENCODING 4S-LIMONENE SYNTHASE FROM MENTHA SPICATA AND THE GENE ENCODING LIMONENE 1,2 MONOOXYGENASE (LIMB) FROM RHODOCOCCUS ERYTHROPOLIS DCL14. THE YEAST XANTHOPHYLLOMYCES DENDRORHOUS WILL BE ENGINEERED TO IMPROVE ASTAXANTHIN PRODUCTION THROUGH THE OVEREXPRESSION OF THE ORTHOLOG GENES ENCODING PHYTOENE SYNTHASE (CRTYB GENE) AND ASTAXANTHIN SYNTHASE (CRTS GENE). ON THE OTHER HAND, IN ORDER TO OBTAIN ZEAXANTHIN-OVERPRODUCING MUTANTS, A MODIFIED STRAIN OF X. DENDRORHOUS, WHICH ACCUMULATES THE PRECURSOR TERPENOID B-CAROTENE, WILL BE ENGINEERED BY MEANS OF THE OVEREXPRESSION OF SEVERAL ORTHOLOG BIOSYNTHETIC GENES AND THE GENE ENCODING B-CAROTENE C3-HYDROXYLASE (CRTZ GENE) FROM BREVUNDIMONAS SP. SD212. ONCE THESE BIOTECHNOLOGICAL PLATFORMS HAVE BEEN OBTAINED, OPTIMIZATION OF THE PRODUCTION PROCESS IS PROPOSED SO AS TO OBTAIN ENOUGH TITERS FOR FURTHER RECOVERY AND PURIFICATION STEPS. THESE NATURAL TERPENOIDS WILL REPRESENT THE BUILDING BLOCKS FOR THE SUBSEQUENT POLYMERIZATION, A PROCESS THAT WILL BE CARRIED OUT BY THE RESEARCH TEAM OF SUBPROJECT 1. (English)
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León
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Identifiers
RTI2018-094840-B-C33
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