No label defined (Q3152667)
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Project Q3152667 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | No label defined |
Project Q3152667 in Spain |
Statements
203,280.0 Euro
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254,100.0 Euro
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80.0 percent
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1 January 2014
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31 December 2017
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AGENCIA CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
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43°28'52.79"N, 5°26'0.46"W
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33076
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LA BILIS ES UN FLUIDO ELABORADO EN EL HIGADO Y ALMACENADO EN LA VESICULA BILIAR, DESDE DONDE SE LIBERA AL DUODENO DURANTE LA DIGESTION DE LOS ALIMENTOS. ENTRE SUS CONSTITUYENTES PRINCIPALES SE ENCUENTRAN LAS SALES BILIARES Y EL COLESTEROL. EN CONDICIONES FISIOLOGICAS NORMALES, LAS SALES BILIARES ACTUAN COMO DETERGENTES BIOLOGICOS Y SOLUBILIZAN LOS COMPUESTOS LIPOFILICOS EN EL INTESTINO, FAVORECIENDO ASI LA DIGESTION DE GRASAS PROCEDENTES DE LA DIETA. SIN EMBARGO, SE HAN DESCRITO ALGUNOS TRASTORNOS BILIARES QUE DIFICULTAN CIERTAS FUNCIONES FISIOLOGICAS DEL ORGANISMO, SIENDO EL MAS HABITUAL LA FORMACION DE CALCULOS BILIARES O LITIASIS._x000D_ LA MICROBIOTA DEL SISTEMA GASTROINTESTINAL HUMANO Y SU RELACION CON DIFERENTES ESTADOS FISIOLOGICOS (DIETA, EDAD, TRASTORNOS METABOLICOS, ETC.) HA SIDO OBJETO DE NUMEROSOS ESTUDIOS DE GRAN IMPACTO CIENTIFICO DURANTE LA ULTIMA DECADA. ASI, LAS POBLACIONES MICROBIANAS DEL ESTOMAGO E INTESTINO HAN SIDO CARACTERIZADAS CON DETALLE. SIN EMBARGO, LA MICROBIOTA DE LA BILIS Y DE LA VESICULA BILIAR APENAS HA SIDO ESTUDIADA HASTA EL MOMENTO, DEBIDO FUNDAMENTALMENTE A LAS DIFICULTADES EXISTENTES PARA ACCEDER A MATERIAL BIOLOGICO Y A LA FALTA DE TECNICAS MOLECULARES ADECUADAS. ADEMAS, LAS FUNCIONES DE LOS MICROORGANISMOS AUTOCTONOS DE LA BILIS Y LAS CARACTERISTICAS QUE LES PERMITEN SOBREVIVIR Y COLONIZAR EL AMBIENTE BILIAR NO HAN SIDO INVESTIGADAS. ESTAS FUNCIONES HAN DE SER, NECESARIAMENTE, EL REFLEJO DE UNA FISIOLOGIA MICROBIANA ADAPTADA A UN AMBIENTE MUY SINGULAR._x000D_ EN ESTE CONTEXTO, CON EL PRESENTE PROYECTO QUEREMOS ABORDAR UN ESTUDIO QUE CONTRIBUYA A ENTENDER LAS FUNCIONES DE LA MICROBIOTA BILIAR HUMANA Y A DETERMINAR SU RELACION CON LA DIETA Y CON ALGUNOS TRASTORNOS BILIARES. PRETENDEMOS CARACTERIZAR NUEVOS BIOMARCADORES DE LITIASIS Y ENZIMAS CON POTENCIAL APLICACION BIOMEDICA Y BIOTECNOLOGICA. PARA ELLO, UTILIZANDO TECNICAS METAPROTEOMICAS Y DE SECUENCIACION MASIVA DE ADN, SE DETERMINARAN LOS PERFILES DE LA MICROBIOTA BILIAR DE INDIVIDUOS CON Y SIN LITIASIS, ASI COMO LOS PERFILES MICROBIANOS DE CALCULOS BILIARES Y DE BIOPSIAS DE LA VESICULA BILIAR. SE ESTABLECERAN CORRELACIONES ENTRE EL METABOLOMA DE LAS MUESTRAS DE BILIS Y LA MICROBIOTA, LA DIETA Y LAS DIFERENTES CONDICIONES FISIOLOGICAS ESTUDIADAS. GENERAREMOS TAMBIEN UNA COLECCION DE MICROORGANISMOS DEL AMBIENTE BILIAR Y LOS MAS REPRESENTATIVOS SERAN CARACTERIZADOS MEDIANTE GENOMICA FUNCIONAL. ADEMAS, PRETENDEMOS DESCUBRIR NUEVAS ACTIVIDADES BACTERIANAS CAPACES DE INTERFERIR EN LA FORMACION DE CALCULOS BILIARES O DE DISGREGARLOS. POR ULTIMO, UNA APROXIMACION TRANSCRIPTOMICA NOS PERMITIRA ESCLARECER LOS MECANISMOS DE CROSS-TALK ENTRE CIERTOS MICROORGANISMOS SELECCIONADOS Y ALGUNOS FACTORES DEL AMBIENTE BILIAR. (Spanish)
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BILE IS A FLUID MADE IN THE LIVER AND STORED IN THE GALL BLADDER, FROM WHICH IT IS RELEASED TO THE DUODENUM DURING FOOD DIGESTION. AMONG ITS MAIN CONSTITUENTS ARE BILE SALTS AND CHOLESTEROL. UNDER NORMAL PHYSIOLOGICAL CONDITIONS, BILE SALTS ACT AS BIOLOGICAL DETERGENTS AND SOLUBILISE LIPOPHILIC COMPOUNDS IN THE INTESTINE, THUS FAVORING THE DIGESTION OF FATS FROM THE DIET. However, there have been some very few BILIARY TRASTORS that divide the physiological functions of the organisation, being the most able to build up of BILIARES or LITIASIS._x000D_ THE MICROBIOTE OF THE HUMAN GASTROINTESTINAL SYSTEM AND RELATION TO DIFFERENT PHYSILOGICAL STATES (DIETA, ADA, METABOLIC TRASTORNOS, ETC.) Has been covered by NUMEROSOS STUDIES OF GREAT SCIENTIFIC IMPACT DURING THE ULTIMAD. THUS, THE MICROBIAL POPULATIONS OF THE STOMACH AND INTESTINE HAVE BEEN CHARACTERISED IN DETAIL. HOWEVER, BILE MICROBIOTA AND GALLBLADDER MICROBIOTA HAVE HARDLY BEEN STUDIED SO FAR, MAINLY DUE TO DIFFICULTIES IN ACCESSING BIOLOGICAL MATERIAL AND LACK OF ADEQUATE MOLECULAR TECHNIQUES. IN ADDITION, THE FUNCTIONS OF AUTOCTONE BILE MICROORGANISMS AND THE CHARACTERISTICS THAT ALLOW THEM TO SURVIVE AND COLONISE THE BILIARY ENVIRONMENT HAVE NOT BEEN INVESTIGATED. These FUNCTIONS HAVE TO BE, NECESARY, THE REFLEMENT OF A MICROBIAN PHYSIOLOGY ADAPTED TO A VERY SINGULAR ENVIRONMENT._x000D_ IN THIS CONTEXT, WITH THE PRESENT PROJECT WOULD ABOUT A STUDY THAT CONTENT TO THE FUNCTIONS OF THE BILIAR MICROBIOTA HUMANA AND DETERMINATE YOUR RELATION TO THE DIET AND WITH BILIARY TRASTORS. WE INTEND TO CHARACTERISE NEW BIOMARKERS OF LITHIASIS AND ENZYMES WITH POTENTIAL APPLICATION BIOMEDICINE AND BIOTECHNOLOGY. FOR THIS PURPOSE, USING METAPROTEOMIC TECHNIQUES AND MASSIVE DNA SEQUENCING, THE PROFILES OF THE BILIARY MICROBIOTA OF INDIVIDUALS WITH AND WITHOUT LITHIASIS, AS WELL AS THE MICROBIAL PROFILES OF BILE CALCULUS AND BILE VESICULA BIOPSIES, WILL BE DETERMINED. CORRELATIONS WILL BE ESTABLISHED BETWEEN THE METABOLOMA OF THE BILE SAMPLES AND THE MICROBIOTA, THE DIET AND THE DIFFERENT PHYSIOLOGICAL CONDITIONS STUDIED. WE WILL ALSO GENERATE A COLLECTION OF MICROORGANISMS FROM THE BILIARY ENVIRONMENT AND THE MOST REPRESENTATIVE ONES WILL BE CHARACTERISED BY FUNCTIONAL GENOMICS. IN ADDITION, WE INTEND TO DISCOVER NEW BACTERIAL ACTIVITIES CAPABLE OF INTERFERING IN THE FORMATION OF BILE CALCULUS OR DISINTEGRATING THEM. FINALLY, A TRANSCRIPTOMIC APPROACH WILL ALLOW US TO CLARIFY THE MECHANISMS OF CROSS-TALK BETWEEN SELECTED MICROORGANISMS AND SOME FACTORS OF THE BILIARY ENVIRONMENT. (English)
12 October 2021
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Villaviciosa
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Identifiers
AGL2013-44761-P
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