Understanding Bacterial Nucleotide precision Repair at the Level of the Single Window Inside Living Cells. (Q84178)
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Project in Poland financed by DG Regio
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | Understanding Bacterial Nucleotide precision Repair at the Level of the Single Window Inside Living Cells. |
Project in Poland financed by DG Regio |
Statements
2,949,970.0 zloty
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2,949,970.0 zloty
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100.0 percent
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1 November 2016
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30 April 2020
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UNIWERSYTET IM. ADAMA MICKIEWICZA W POZNANIU
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Malfunctioning DNA repair lead to an accumulation of mutations, which frequently results in cancer. The Nucleotide Excision Repair (NER) pathway removes a DNA lesions caused by UV light, cigarette smoke and chemical mutagens. NER is highly conserved, and studying the simpler NER in bacteria provides key insight into human NER. I propose an interdisciplinary approach to understand the mechanistic details of bacterial NER in living cells. I will use a combination of cutting-edge single-molecule methods to elucidate how DNA is repaired inside living cells. I will use super-resolution microscopy combined to study the behaviour of individual NER proteins. To complement this, conventional biochemistry, cell biology, genetics, smFRET assays, FCS and TIRF microscopy will be used. Together, this will provide a comprehensive understanding of the bacterial NER pathway, and constitute the first steps toward my ultimate goal, which is to understand how human cells repair DNA. (Polish)
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Malfuntioning DNA repairs extended to an accumulation of walls, which have their direct results in cancer.The Nucleotide precision Repairs (NER) path response to the DNA caused by UV light, smoke suppression and chemical mutagens.NER is hargly collected, and studying the simpler NER in shepherd with human right into human NER.And the proposal for an Interdisciplinary approach to understane the mechanistic details of shepherd’s bacterial in living cells.And will use a combination of methods.And will use super-resolution microcopes combined.This is to be completed this, vertical biochemical cell, cell biotics, smPRET Assays, FCS and TIRF mix will be used.Together, this will help a comprehensive view of the bacterial, and constitute the first steps towards ultimate steppe DNA. (English)
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Identifiers
POIR.04.04.00-00-1CA9/16
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