SURVIVAL CHARACTERS IN THE INTESTINAL NICHE OF BACTERIA FROM THE TAXON LACTOBACILLUS CASEI/PARACASEI/RHAMNOSUS AS CRITERIA FOR THE SELECTION OF PROBIOTICS (Q3167765)
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Project Q3167765 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | SURVIVAL CHARACTERS IN THE INTESTINAL NICHE OF BACTERIA FROM THE TAXON LACTOBACILLUS CASEI/PARACASEI/RHAMNOSUS AS CRITERIA FOR THE SELECTION OF PROBIOTICS |
Project Q3167765 in Spain |
Statements
66,550.0 Euro
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133,100.0 Euro
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50.0 percent
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1 January 2014
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31 December 2015
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AGENCIA CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
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39°30'14.11"N, 0°26'31.56"W
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46190
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LAS BACTERIAS INTESTINALES HAN DESARROLLADO RECURSOS PARA ADHERIRSE A LA MUCOSA Y EVITAR LAS AMENAZAS DEL SISTEMA INMUNE; ALGUNOS DE ELLOS SON COMPARTIDOS POR PATOGENOS Y ESPECIES MUTUALISTAS. POR DEFINICION, LAS BACTERIAS MUTUALISTAS PARTICIPAN EN UNA RELACION DE MUTUO BENEFICIO CON EL HUESPED QUE IMPLICA HOMEOSTASIS DEL PROCESO DE FERMENTACION DE LOS ALIMENTOS, DIGESTIBILIDAD Y PROVISION DE NUTRIENTES O COMPETENCIA CON PATOGENOS Y, RECIENTEMENTE SE HAN RELACIONADO CON EL MANTENIMIENTO DE LA HOMEOSTASIS DEL SISTEMA INMUNE INTESTINAL Y QUIZA CON SU APROPIADA MADURACION DURANTE LA INFANCIA. LOS PROBIOTICOS YA UTILIZADOS, ASI COMO LOS FUTUROS, SON AISLADOS GENERALMENTE DE MUESTRAS FECALES. DEBEN POR TANTO SER TRATADOS COMO CONSTITUYENTES MUTUALISTAS DE LA MICROBIOTA INTESTINAL Y SUS PROPIEDADES ESTUDIADAS EN EL CONTEXTO DEL NICHO ECOLOGICO INTESTINAL. EN UN PROYECTO PREVIO (AGL2010-22211) CARACTERIZAMOS PROTEINAS DE L.CASEI QUE SE UNEN A LA MATRIZ EXTRACELULAR. RESULTARON SER ENZIMAS GLUCLOLITICOS, FACTORES DE TRANSCRIPCION Y OTRAS PROTEINAS DE SUPERFICIE NO CONVENCIONALES (NCSPS), ASI COMO UNA PROTEINA DE UNION A FIBRONECTINA (FBPA), COMO EN OTROS PROBIOTICOS. TAMBIEN CARACTERIZAMOS MURAMINIDASAS DE PARED CELULAR DE L.CASEI, IDENTIFICADAS EN L. RHAMOSUS GG COMO PROTEINAS QUE PREVIENEN EL DAÑO POR ESTRES Y POR CITOQUINAS, Y APOPTOSIS (P40 Y P75). ADEMAS, CONTRIBUIMOS A ESTUDIAR EL PAPEL DE PRTP COMO EL FACTOR QUE RESPONSABLE DEL EFECTO ANTIINFLAMATORIO DEL SOBRENADANTE DE L.CASEI VSL#3. ESTE PROYECTO ESTUDIARA LA INTERACCION ENTRE LAS PROTEINAS P40, P75, FBPA Y ENZIMAS GLUCLITICOS COMO LA ENOLASA (ENO1) Y LA GLICERALDEHIDO-3P DESHIDROGENASA (GAPDH) DEL GRUPO L. CASEI/ RHAMNOSUS, CON LAS CELULAS EPITELIALES. ABORDAREMOS LA CARACTERIZACION DE RECEPTORES Y DE CONEXIONES CON LAS RUTAS DE TRANSDUCCION DE SEÑAL P38 MAPK-EGFR, EN EL CASO DE P40 Y P75, Y TNF-ALPHA/ NF-KB EN LAS NCSPS. FINALMENTE, ESTUDIAREMOS EL EFECTO IN VIVO DE LAS NCSP EN UN MODELO DE RATON EN EL QUE TAMBIEN SE ANALIZARAN LAS PROTEINAS LIBRES EN SOBRENADANTE DE HECES MEDIANTE UNA APROXIMACION PROTEOMICA. MEDIANTE LOS EXPERIMENTOS DISEÑADOS APORTAREMOS EVIDENCIAS DE LOS MECANISMOS MOLECULARES QUE SUBYACEN EN LA ARCAICA COMUNICACION ENTRE LA MUCOSA INTESTINAL DEL HUESPED Y LAS BACTERIAS MUTUALISTAS Y CONSOLIDAREMOS EL MECANISMO DE ACCION DE LOS PROBIOTICOS. NUESTRO OBJETIVO ES REALIZAR UNA APORTACION EN UN CAMPO DE INVESTIGACION (MICROBIOTA-SALUD) CON APLICACIONES INMEDIATAS HACIA PRODUCTOS COMERCIALES, PERO QUE TAMBIEN TIENE GRAN RELEVANCIA A LARGO PLAZO PARA EL MANTENIMIENTO DEL BIENESTAR INDIVIDUAL Y EN SALUD COMUNITARIA, PUES PUEDE CONTRIBUIR A REDUCIR EL CONSUMO DE MEDICAMENTOS Y DEL GASTO EN EL SISTEMA PUBLICO DE SALUD. (Spanish)
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INTESTINAL BACTERIA HAVE DEVELOPED RESOURCES TO ADHERE TO THE MUCOSA AND AVOID THREATS FROM THE IMMUNE SYSTEM; SOME OF THEM ARE SHARED BY PATHOGENS AND MUTUALISTIC SPECIES. BY DEFINITION, THE MUTUALISTIC BACTERIA PARTICIPATE IN A MUTUALLY BENEFICIAL RELATIONSHIP WITH THE HOST THAT INVOLVES HOMEOSTASIS OF THE PROCESS OF FERMENTATION OF FOOD, DIGESTIBILITY AND NUTRIENT PROVISION OR COMPETITION WITH PATHOGENS AND, RECENTLY, HAVE BEEN RELATED TO THE MAINTENANCE OF THE HOMEOSTASIS OF THE INTESTINAL IMMUNE SYSTEM AND PERHAPS WITH ITS PROPER MATURATION DURING CHILDHOOD. PROBIOTICS ALREADY USED, AS WELL AS FUTURE ONES, ARE USUALLY ISOLATED FROM FAECAL SAMPLES. THEY MUST THEREFORE BE TREATED AS MUTUAL CONSTITUENTS OF THE INTESTINAL MICROBIOTA AND ITS PROPERTIES STUDIED IN THE CONTEXT OF THE INTESTINAL ECOLOGICAL NICHE. IN A PREVIOUS PROJECT (AGL2010-22211) WE CHARACTERISED L.CASEI PROTEINS THAT BIND TO THE EXTRACELLULAR MATRIX. THEY TURNED OUT TO BE GLUCLOLITIC ENZYMES, TRANSCRIPTION FACTORS AND OTHER UNCONVENTIONAL SURFACE PROTEINS (NCSPS), AS WELL AS A UNION-TO-FIBRONECTIN PROTEIN (FBPA), AS IN OTHER PROBIOTICS. WE ALSO CHARACTERISE L.CASEI CELL WALL MURAMINIDAS, IDENTIFIED IN L. RHAMOSUS GG AS PROTEINS THAT PREVENT DAMAGE BY STRESS AND CYTOKINES, AND APOPTOSIS (P40 AND P75). IN ADDITION, WE CONTRIBUTE TO STUDYING THE ROLE OF PRTP AS THE FACTOR RESPONSIBLE FOR THE ANTI-INFLAMMATORY EFFECT OF L.CASEI VSL#3 SUPERNATANT. THIS PROJECT WILL STUDY THE INTERACTION BETWEEN PROTEINS P40, P75, FBPA AND GLUCLITIC ENZYMES SUCH AS ENOLASE (ENO1) AND GLYCERALDEHYDE-3P DEHYDROGENASE (GAPDH) OF THE L. CASEI/RHAMNOSUS GROUP WITH EPITHELIAL CELLS. WE WILL ADDRESS THE CHARACTERISATION OF RECEIVERS AND CONNECTIONS WITH THE SIGNAL TRANSDUCTION ROUTES P38 MAPK-EGFR, IN THE CASE OF P40 AND P75, AND TNF-ALPHA/NF-KB IN NCSPS. FINALLY, WE WILL STUDY THE IN VIVO EFFECT OF NCSPS IN A RATON MODEL IN WHICH FREE PROTEINS IN FECES SUPERNATANT WILL ALSO BE ANALYSED USING A PROTEOMIC APPROACH. THROUGH THE EXPERIMENTS DESIGNED WE WILL PROVIDE EVIDENCE OF THE MOLECULAR MECHANISMS THAT UNDERLIE THE ARCHAIC COMMUNICATION BETWEEN THE INTESTINAL MUCOSA OF THE HOST AND THE MUTUALISTIC BACTERIA AND WE WILL CONSOLIDATE THE MECHANISM OF ACTION OF THE PROBIOTICS. OUR OBJECTIVE IS TO MAKE A CONTRIBUTION IN A FIELD OF RESEARCH (MICROBIOTA-HEALTH) WITH IMMEDIATE APPLICATIONS TO COMMERCIAL PRODUCTS, BUT WHICH IS ALSO OF GREAT LONG-TERM RELEVANCE FOR THE MAINTENANCE OF INDIVIDUAL WELL-BEING AND IN COMMUNITY HEALTH, AS IT CAN CONTRIBUTE TO REDUCING THE CONSUMPTION OF MEDICINES AND SPENDING ON THE PUBLIC HEALTH SYSTEM. (English)
12 October 2021
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Paterna
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Identifiers
AGL2013-47420-R
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