Study of sST2 as a therapeutic target to prevent adverse cardiac remodeling after acute myocardial infarction (Q3179275): Difference between revisions
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(Created claim: summary (P836): Scenario: The identification of the elements involved in sST2 modulation in AMI will allow us to develop strategies to decrease the expression of sST2 and slow progression of adverse myocardiac remodeling after AMI. Objectives and Methods: 1) Determine the tissues and cell types responsible for the synthesis of sST2 in response to AMI, by (a) measuring the transcardiac gradient of sST2 between arterial blood and coronary sinus in patients with A...) |
(Changed label, description and/or aliases in en: translated_label) |
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Study of sST2 as a therapeutic target to prevent adverse cardiac remodeling after acute myocardial infarction | |||
Revision as of 19:02, 12 October 2021
Project Q3179275 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | Study of sST2 as a therapeutic target to prevent adverse cardiac remodeling after acute myocardial infarction |
Project Q3179275 in Spain |
Statements
47,600.0 Euro
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59,500.0 Euro
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80.0 percent
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1 January 2015
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31 March 2019
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FUNDACION PARA LA FORMACION E INVESTIGACION SANITARIAS DE LA REGION DE MURCIA (FFIS)
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37°59'32.57"N, 1°7'49.94"W
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30030
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Hipótesis: La identificación de los elementos que participan en la modulación del sST2 en el IAM nos permitirá desarrollar estrategias para disminuir la expresión de sST2 y frenar la progresión del remodelado miocardiaco adverso tras IAM. Objetivos y Métodos: 1) Determinar los tejidos y tipos celulares responsables de la síntesis de sST2 en respuesta al IAM, mediante (a) la medida del gradiente trascardiaco de sST2 entre sangre arterial y seno coronario en pacientes con IAM y (b) el estudio diferencial de la expresión génica de sST2 en muestras tisulares, obtenidas a partir de un modelo experimental de IAM en ratón, usando técnicas de RT-PCR a tiempo real, transferencia Western así como la identificación y colocalización in situ del sST2 ARNm en el tejido, con diferentes marcadores celulares. 2) Identificar los factores de transcripción y los elementos de regulación de splicing alternativo implicados en la sobre-expresión de sST2, mediante el uso de líneas celulares y cultivos primarios obtenidos a partir de tejido fresco, sometidos a estrés biomecánico simulado. Se emplearán técnicas de RT-PCR a tiempo real, ELISA, así como modelos Knockdown mediante el uso de siRNAs específicos. 3) Evaluar la inhibición de la expresión de sST2 como terapia frente al remodelado miocárdico post-IAM, mediante el uso de un modelo animal de IAM y técnicas de silenciamiento génico con ARN interferente. (Spanish)
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Scenario: The identification of the elements involved in sST2 modulation in AMI will allow us to develop strategies to decrease the expression of sST2 and slow progression of adverse myocardiac remodeling after AMI. Objectives and Methods: 1) Determine the tissues and cell types responsible for the synthesis of sST2 in response to AMI, by (a) measuring the transcardiac gradient of sST2 between arterial blood and coronary sinus in patients with AMI and (b) differential study of the gene expression of sST2 in tissue samples, obtained from an experimental model of mouse AMI, using real-time RT-PCR techniques, Western transfer as well as the identification and placement in situ of sST2 mRNA in the tissue, with different cell markers. 2) Identify the transcription factors and the elements of regulation of alternative splicing involved in the over-expression of sST2, using cell lines and primary cultures obtained from fresh tissue, subjected to simulated biomechanical stress. Real-time RT-PCR techniques, ELISA, as well as Knockdown models will be used using specific siRNAs. 3) Evaluate inhibition of sST2 expression as therapy against post-AMI myocardial remodeling, using an animal model of AMI and gene silencing techniques with interfering RNA. (English)
12 October 2021
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Murcia
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Identifiers
PI14_01637
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