Q3136959 (Q3136959): Difference between revisions
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(Created claim: summary (P836): Background: The mechanisms of action underlying the improvement/resolution of comorbidities after bariatric surgery (CB) are not well known. Scenario: The present draft assumes that the effects of CB are not only due to weight loss, but some comorbidities depend directly on the reduction of dysfunctional adiposity, which does not decrease in parallel to body weight. Thus, alterations in the expression profile of inflammatory genes and extracellu...) |
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Background: The mechanisms of action underlying the improvement/resolution of comorbidities after bariatric surgery (CB) are not well known. Scenario: The present draft assumes that the effects of CB are not only due to weight loss, but some comorbidities depend directly on the reduction of dysfunctional adiposity, which does not decrease in parallel to body weight. Thus, alterations in the expression profile of inflammatory genes and extracellular matrix underlying changes in plasticity and biomechanical properties of white adipose tissue (BTA) could be determinants. Objectives and methodology: Body composition studies (Bod-Pod and Viscan) will be conducted in a prospective study of obese patients (n=120) undergoing CB and 20 normoponderal controls undergoing Nissen funduplication. Pre-surgical data shall be compared with those obtained at 6, 12, 24 and 36 months after CB; the clinical study will be complemented by the mechanistic deepening in an experimental model consisting of 120 rats distributed in 12 groups (control vs. diet-induced obesity), surgical manipulation (sham vs CB), form of caloric restriction (surgical vs pair-fed) and temporal pattern (short/medium/long term). These analyses in patients and rats will be accompanied by histologic, immunohistochemical, biomechanical, gene and protein expression studies of subcutaneous and visceral TAB of inflammatory markers (CCL2, CXCL1, CXCL8, IL-1b, IL-1a, IL-1RN, IL-4, IL-6, IL-18 and TNF-a). as well as hypoxia, angiogenesis and remodeling of the extracellular matrix (elastine, CTGF, TNMD, MMP2, MMP9, TGF-b, TNC, VEGFA) in order to show that changes in the plasticity and functionality of TAB are related to the temporal pattern of resolution of inflammation and insulin. (English) | |||||||||||||||
| Property / summary: Background: The mechanisms of action underlying the improvement/resolution of comorbidities after bariatric surgery (CB) are not well known. Scenario: The present draft assumes that the effects of CB are not only due to weight loss, but some comorbidities depend directly on the reduction of dysfunctional adiposity, which does not decrease in parallel to body weight. Thus, alterations in the expression profile of inflammatory genes and extracellular matrix underlying changes in plasticity and biomechanical properties of white adipose tissue (BTA) could be determinants. Objectives and methodology: Body composition studies (Bod-Pod and Viscan) will be conducted in a prospective study of obese patients (n=120) undergoing CB and 20 normoponderal controls undergoing Nissen funduplication. Pre-surgical data shall be compared with those obtained at 6, 12, 24 and 36 months after CB; the clinical study will be complemented by the mechanistic deepening in an experimental model consisting of 120 rats distributed in 12 groups (control vs. diet-induced obesity), surgical manipulation (sham vs CB), form of caloric restriction (surgical vs pair-fed) and temporal pattern (short/medium/long term). These analyses in patients and rats will be accompanied by histologic, immunohistochemical, biomechanical, gene and protein expression studies of subcutaneous and visceral TAB of inflammatory markers (CCL2, CXCL1, CXCL8, IL-1b, IL-1a, IL-1RN, IL-4, IL-6, IL-18 and TNF-a). as well as hypoxia, angiogenesis and remodeling of the extracellular matrix (elastine, CTGF, TNMD, MMP2, MMP9, TGF-b, TNC, VEGFA) in order to show that changes in the plasticity and functionality of TAB are related to the temporal pattern of resolution of inflammation and insulin. (English) / rank | |||||||||||||||
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| Property / summary: Background: The mechanisms of action underlying the improvement/resolution of comorbidities after bariatric surgery (CB) are not well known. Scenario: The present draft assumes that the effects of CB are not only due to weight loss, but some comorbidities depend directly on the reduction of dysfunctional adiposity, which does not decrease in parallel to body weight. Thus, alterations in the expression profile of inflammatory genes and extracellular matrix underlying changes in plasticity and biomechanical properties of white adipose tissue (BTA) could be determinants. Objectives and methodology: Body composition studies (Bod-Pod and Viscan) will be conducted in a prospective study of obese patients (n=120) undergoing CB and 20 normoponderal controls undergoing Nissen funduplication. Pre-surgical data shall be compared with those obtained at 6, 12, 24 and 36 months after CB; the clinical study will be complemented by the mechanistic deepening in an experimental model consisting of 120 rats distributed in 12 groups (control vs. diet-induced obesity), surgical manipulation (sham vs CB), form of caloric restriction (surgical vs pair-fed) and temporal pattern (short/medium/long term). These analyses in patients and rats will be accompanied by histologic, immunohistochemical, biomechanical, gene and protein expression studies of subcutaneous and visceral TAB of inflammatory markers (CCL2, CXCL1, CXCL8, IL-1b, IL-1a, IL-1RN, IL-4, IL-6, IL-18 and TNF-a). as well as hypoxia, angiogenesis and remodeling of the extracellular matrix (elastine, CTGF, TNMD, MMP2, MMP9, TGF-b, TNC, VEGFA) in order to show that changes in the plasticity and functionality of TAB are related to the temporal pattern of resolution of inflammation and insulin. (English) / qualifier | |||||||||||||||
point in time: 12 October 2021
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Revision as of 13:41, 12 October 2021
Project Q3136959 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | No label defined |
Project Q3136959 in Spain |
Statements
107,625.0 Euro
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215,250.0 Euro
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50.0 percent
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1 January 2017
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31 March 2020
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UNIVERSIDAD DE NAVARRA
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42°49'6.42"N, 1°38'39.34"W
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31201
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Antecedentes: Los mecanismos de acción subyacentes a la mejoría/resolución de comorbilidades tras cirugía bariátrica (CB) no son bien conocidos. Hipótesis: El presente proyecto plantea la hipótesis de que los efectos de la CB no se deben sólo a la pérdida ponderal, sino que algunas comorbilidades dependen directamente de la reducción de la adiposidad disfuncional, que no disminuye en paralelo al peso corporal. Así, alteraciones en el perfil de expresión de genes inflamatorios y de la matriz extracelular subyacentes a cambios en la plasticidad y las propiedades biomecánicas del tejido adiposo blanco (TAB) podrían ser determinantes. Objetivos y metodología: Se realizarán estudios de composición corporal (Bod-Pod y ViScan) en un estudio prospectivo de pacientes obesos (n=120) sometidos a CB y 20 controles normoponderales sometidos a funduplicatura de Nissen. Se compararán los datos prequirúrgicos y los obtenidos a los 6, 12, 24 y 36 meses de la CB; se correlacionarán con la evolución ponderal y los niveles circulantes de adipoquinas y biomarcadores indicativos de inflamación y fibrosis del TAB. El estudio clínico se complementará con la profundización mecanística en un modelo experimental constituido por 120 ratas distribuidas en 12 grupos (control vs obesidad inducida por dieta), manipulación quirúrgica (sham vs CB), forma de restricción calórica (quirúrgica vs pair-fed) y patrón temporal (corto/medio/largo plazo). Estos análisis en pacientes y ratas se acompañarán de estudios histológicos, immunohistoquímicos, biomecánicos, de expresión génica y proteica del TAB subcutáneo y visceral de marcadores inflamatorios (CCL2, CXCL1, CXCL8, IL-1b, IL-1a, IL-1RN, IL-4, IL-6, IL-18 y TNF-a), así como de hipoxia, angiogénesis y remodelado de la matriz extracelular (elastina, CTGF, TNMD, MMP2, MMP9, TGF-b, TNC, VEGFA) con objeto de evidenciar que los cambios en la plasticidad y funcionalidad del TAB se relacionan con el patrón temporal de resolución de inflamación e insulino- (Spanish)
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Background: The mechanisms of action underlying the improvement/resolution of comorbidities after bariatric surgery (CB) are not well known. Scenario: The present draft assumes that the effects of CB are not only due to weight loss, but some comorbidities depend directly on the reduction of dysfunctional adiposity, which does not decrease in parallel to body weight. Thus, alterations in the expression profile of inflammatory genes and extracellular matrix underlying changes in plasticity and biomechanical properties of white adipose tissue (BTA) could be determinants. Objectives and methodology: Body composition studies (Bod-Pod and Viscan) will be conducted in a prospective study of obese patients (n=120) undergoing CB and 20 normoponderal controls undergoing Nissen funduplication. Pre-surgical data shall be compared with those obtained at 6, 12, 24 and 36 months after CB; the clinical study will be complemented by the mechanistic deepening in an experimental model consisting of 120 rats distributed in 12 groups (control vs. diet-induced obesity), surgical manipulation (sham vs CB), form of caloric restriction (surgical vs pair-fed) and temporal pattern (short/medium/long term). These analyses in patients and rats will be accompanied by histologic, immunohistochemical, biomechanical, gene and protein expression studies of subcutaneous and visceral TAB of inflammatory markers (CCL2, CXCL1, CXCL8, IL-1b, IL-1a, IL-1RN, IL-4, IL-6, IL-18 and TNF-a). as well as hypoxia, angiogenesis and remodeling of the extracellular matrix (elastine, CTGF, TNMD, MMP2, MMP9, TGF-b, TNC, VEGFA) in order to show that changes in the plasticity and functionality of TAB are related to the temporal pattern of resolution of inflammation and insulin. (English)
12 October 2021
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Pamplona/Iruña
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Identifiers
PI16_01217
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