Study of H2S biosynthesis in human adipose tissue and its possible effect on adipogenesis and insulin sensitivity (Q3136840): Difference between revisions
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(Created claim: summary (P836): Experimental studies in mice and nutritional studies suggest a hydrogen sulfide (H2S) role in glucose metabolism and adipocyte physiology. In the best of our knowledge, there are no previous studies investigating the effect of H2S on the physiology of human adipose tissue and adipocyte. Preliminary data obtained in our laboratory demonstrate significant levels of key enzymes in endogenous biosynthesis of H2S in human adipose tissue, and specific...) |
(Changed label, description and/or aliases in en: translated_label) |
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Study of H2S biosynthesis in human adipose tissue and its possible effect on adipogenesis and insulin sensitivity | |||
Revision as of 13:40, 12 October 2021
Project Q3136840 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | Study of H2S biosynthesis in human adipose tissue and its possible effect on adipogenesis and insulin sensitivity |
Project Q3136840 in Spain |
Statements
35,750.0 Euro
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71,500.0 Euro
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50.0 percent
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1 January 2017
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31 March 2020
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CENTRO DE INVESTIGACION BIOMEDICA EN RED (CIBER)
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41°58'45.48"N, 2°49'11.78"E
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17079
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Estudios experimentales en ratones y estudios nutricionales sugieren un papel sulfuro de hidrogeno (H2S) en el metabolismo de la glucosa y en la fisiología del adipocito. En el mejor de nuestro conocimiento, no hay estudios previos que investiguen el efecto del H2S en la fisiología del tejido adiposo y adipocito humano. Datos preliminares obtenidos en nuestro laboratorio, demuestran niveles significativos de enzimas clave en la biosíntesis endógena de H2S en tejido adiposo humano, y específicamente en adipocitos (datos no publicados), cosa que sugiere un posible rol de la producción endógena de H2S en la fisiología del tejido adiposo humano, y en consecuencia en el desarrollo de los trastornos metabólicos asociados a obesidad. Con el objetivo de investigar esta hipótesis, este proyecto plantea estudiar: i) los niveles de H2S producidos en el tejido adiposo y presentes en suero de pacientes con diferentes grados de obesidad y sensibilidad a la insulina, y en células humanas durante la diferenciación adipocitaria; ii) la expresión de las proteínas implicadas en la producción de H2S (incluyendo CTH, CBS, MPST, TST y NFS1) en el tejido adiposo y durante la diferenciación adipocitaria; iii) funcionalmente el efecto del H2S en la adipogenesis, inflamación, sensibilidad a la insulina y captación de glucosa, y función mitocondrial en adipocitos humanos. Primero, se procederá a la medición de H2S en el tejido adiposo y suero, y al procesamiento del tejido adiposo y, lo que incluye la purificación y análisis de ARN y proteínas. Seguidamente, se examinará in vitro, la biosíntesis de H2S durante la diferenciación adipocitaria, y diferentes condiciones de inflamación y resistencia a la insulina. Posteriormente, se realizarán experimentos funcionales in vitro, testando los efectos de la administración de H2S e inhibidores de la biosíntesis de H2S. (Spanish)
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Experimental studies in mice and nutritional studies suggest a hydrogen sulfide (H2S) role in glucose metabolism and adipocyte physiology. In the best of our knowledge, there are no previous studies investigating the effect of H2S on the physiology of human adipose tissue and adipocyte. Preliminary data obtained in our laboratory demonstrate significant levels of key enzymes in endogenous biosynthesis of H2S in human adipose tissue, and specifically in adipocytes (unpublished data), suggesting a possible role of endogenous H2S production in human adipose tissue physiology, and consequently in the development of metabolic disorders associated with obesity. In order to investigate this hypothesis, this project proposes to study: the levels of H2S produced in adipose tissue and present in serum in patients with varying degrees of obesity and insulin sensitivity, and in human cells during adipocity differentiation; the expression of proteins involved in the production of H2S (including CTH, CBS, MPST, TST and NFS1) in adipose tissue and during adipocity differentiation; III) functionally the effect of H2S on adipogenesis, inflammation, insulin sensitivity and glucose uptake, and mitochondrial function in human adipocytes. First, the measurement of H2S in the adipose tissue and serum, and the processing of adipose tissue and, including purification and analysis of RNA and proteins, will be carried out. The biosynthesis of H2S during adipocity differentiation and different conditions of inflammation and insulin resistance will then be examined in vitro. Subsequently, in vitro functional experiments will be performed, testing the effects of H2S administration and H2S biosynthesis inhibitors. (English)
12 October 2021
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Girona
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Identifiers
PI16_01173
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