Identification of chromium-bearing mechanical crossover formation in plants (Q84329): Difference between revisions
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(Removed claim: summary (P836): Meotitic cross is a fundamental process that shows and is to be imported substrate for crop breding.In this proposal.First, in the view of a new approach in their will allow us is a study selected by the Commission.This will be based on ReCombinant seed sorted byNext, in the will direct test effects of two types of chromatids on cross over distribution:hyphone H3 trimethylation at lysine 4 (H3K4me3), and hisone H4 acetylation.For this purpose,...) |
(Created claim: summary (P836): Meiotic crossover is a fundamental process that unlocks and generates genetic diversity and is therefore an important substrate for crop breeding. In this proposal, we seek to characterise the chromatin factors controlling meiotic crossover in plant genomes. First, we will develop a new approach which will allow us to study selected crossover hotspots in Arabidopsis with high resolution. This will be based on recombinant seed sorting with fluore...) |
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Meiotic crossover is a fundamental process that unlocks and generates genetic diversity and is therefore an important substrate for crop breeding. In this proposal, we seek to characterise the chromatin factors controlling meiotic crossover in plant genomes. First, we will develop a new approach which will allow us to study selected crossover hotspots in Arabidopsis with high resolution. This will be based on recombinant seed sorting with fluorescent Reporters and successive sequencing of crossover-containing intervals. Next, we will directly test effects of two types of chromatin modification on crossover distribution: histone H3 trimethylation at lysine 4 (H3K4me3), and histone H4 acetylation. For this purpose, we will target histone modifying enzymes to crossover hotspots within our intervals by CRISPR/dCas9 system. This project will lead to new discoveries on the crossover control and will pave a way to generate artificial recombination hotspots for modern plant breeding. (English) | |||||||||||||||
| Property / summary: Meiotic crossover is a fundamental process that unlocks and generates genetic diversity and is therefore an important substrate for crop breeding. In this proposal, we seek to characterise the chromatin factors controlling meiotic crossover in plant genomes. First, we will develop a new approach which will allow us to study selected crossover hotspots in Arabidopsis with high resolution. This will be based on recombinant seed sorting with fluorescent Reporters and successive sequencing of crossover-containing intervals. Next, we will directly test effects of two types of chromatin modification on crossover distribution: histone H3 trimethylation at lysine 4 (H3K4me3), and histone H4 acetylation. For this purpose, we will target histone modifying enzymes to crossover hotspots within our intervals by CRISPR/dCas9 system. This project will lead to new discoveries on the crossover control and will pave a way to generate artificial recombination hotspots for modern plant breeding. (English) / rank | |||||||||||||||
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| Property / summary: Meiotic crossover is a fundamental process that unlocks and generates genetic diversity and is therefore an important substrate for crop breeding. In this proposal, we seek to characterise the chromatin factors controlling meiotic crossover in plant genomes. First, we will develop a new approach which will allow us to study selected crossover hotspots in Arabidopsis with high resolution. This will be based on recombinant seed sorting with fluorescent Reporters and successive sequencing of crossover-containing intervals. Next, we will directly test effects of two types of chromatin modification on crossover distribution: histone H3 trimethylation at lysine 4 (H3K4me3), and histone H4 acetylation. For this purpose, we will target histone modifying enzymes to crossover hotspots within our intervals by CRISPR/dCas9 system. This project will lead to new discoveries on the crossover control and will pave a way to generate artificial recombination hotspots for modern plant breeding. (English) / qualifier | |||||||||||||||
point in time: 14 October 2020
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Revision as of 12:36, 14 October 2020
Project in Poland financed by DG Regio
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | Identification of chromium-bearing mechanical crossover formation in plants |
Project in Poland financed by DG Regio |
Statements
3,499,750.0 zloty
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3,499,750.0 zloty
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100.0 percent
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1 December 2018
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30 November 2021
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UNIWERSYTET IM. ADAMA MICKIEWICZA W POZNANIU
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Q2513981 (Deleted Item)
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Meiotic crossover is a fundamental process that unlocks and generates genetic diversity and is therefore an important substrate for crop breeding. In this proposal, we seek to characterize the chromatin factors controlling meiotic crossover in plant genomes. First, we will develop a new approach which will allow us to study selected crossover hotspots in Arabidopsis with high resolution. This will be based on recombinant seed sorting with fluorescent reporters and successive sequencing of crossover-containing intervals. Next, we will directly test effects of two types of chromatin modification on crossover distribution: histone H3 trimethylation at lysine 4 (H3K4me3), and histone H4 acetylation. For this purpose, we will target histone modifying enzymes to crossover hotspots within our intervals by CRISPR/dCas9 system. This project will lead to new discoveries on the crossover control and will pave a way to generate artificial recombination hotspots for modern plant breeding. (Polish)
0 references
Meiotic crossover is a fundamental process that unlocks and generates genetic diversity and is therefore an important substrate for crop breeding. In this proposal, we seek to characterise the chromatin factors controlling meiotic crossover in plant genomes. First, we will develop a new approach which will allow us to study selected crossover hotspots in Arabidopsis with high resolution. This will be based on recombinant seed sorting with fluorescent Reporters and successive sequencing of crossover-containing intervals. Next, we will directly test effects of two types of chromatin modification on crossover distribution: histone H3 trimethylation at lysine 4 (H3K4me3), and histone H4 acetylation. For this purpose, we will target histone modifying enzymes to crossover hotspots within our intervals by CRISPR/dCas9 system. This project will lead to new discoveries on the crossover control and will pave a way to generate artificial recombination hotspots for modern plant breeding. (English)
14 October 2020
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Identifiers
POIR.04.04.00-00-5C0F/17
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