Q3681383 (Q3681383): Difference between revisions
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(Changed label, description and/or aliases in en: Setting new description) |
(Created claim: summary (P836): In the field of material physics transmission electron microscopy, has undergone rapid developments in terms of high resolution and spatially resolved spectroscopy for about ten years. The current electron microscopes are equipped with probe aberration correctors, and efficient spectrometers that allow the use of high resolution scanning modes (STEM), energy loss spectroscopy (EELS), energy dispersion (EDS) and filtered imaging (EFTEM). Thus, th...) |
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In the field of material physics transmission electron microscopy, has undergone rapid developments in terms of high resolution and spatially resolved spectroscopy for about ten years. The current electron microscopes are equipped with probe aberration correctors, and efficient spectrometers that allow the use of high resolution scanning modes (STEM), energy loss spectroscopy (EELS), energy dispersion (EDS) and filtered imaging (EFTEM). Thus, thanks to these technological advances, it is possible to access chemical composition and structural information at atomistic scales and have greatly contributed to increasing the knowledge of the structural-function-properties of inorganic materials. While these high-resolution and chemical imaging approaches are widely applied to the field of material physics, they remain poorly transposed to biological systems, for several reasons: the need for advanced specific instrumentation (high resolution electronic microscopes with analytical configuration) ii) low concentration of elements to be detected, iii) fragility of biological samples subject to electron beam stresses, and finally iv) stability of the elements to be detected during sample preparation processes. Despite these significant technical challenges, the use of chemical imaging modes in biology remains a strong asset in the localisation of identification and visualisation of cellular structures and molecular assemblies, which are key points of many biological questions. By exploiting the different physical properties related to interactions between the electron beam and biological matter, electron microscopy with analytical configurations (META) remains a complementary and unavoidable approach to biochemical, molecular and chemical analyses; they optimise morphological studies at subcellular scales, establish chemical mappings of endogenous or exogenous elements, and thus enhance the contrast of imaging. A true link to the understanding of functional structure relationships, META is an indispensable tool for physio/pathological and toxicological studies. In this context, where chemical imaging remains a strong and innovative asset for biology, the CellSTEM project proposes the implementation of chemical imaging approaches in transmission electron microscopy through scanning modes (STEM), energy loss spectroscopy (EELS/EFTEM) and dispersive energy (EDS) to address as application components an understanding of the cellular mechanisms that lead to the development of cardiovascular and pulmonary pathologies in a dual physiopathological and environmental context. With more than 17,5 million deaths/year, cardiovascular disease is the world’s leading cause of death (WHO, 2012) with 4 out of 5 deaths from myocardial infarction. Encouraged by behavioural risks (smoking, poor diet obesity, installed diseases) they are increased by environmental factors (air pollution, noise stress and constitute a major health concern by the public authorities. The CellSTEM project based on META inputs will focus on assessing ultrastructural changes 1) endothelial and cardiac cells in different pathological contexts, and 2) endothelial and lung cells in a context of exposure to environmental stress. (English) | |||||||||||||||
| Property / summary: In the field of material physics transmission electron microscopy, has undergone rapid developments in terms of high resolution and spatially resolved spectroscopy for about ten years. The current electron microscopes are equipped with probe aberration correctors, and efficient spectrometers that allow the use of high resolution scanning modes (STEM), energy loss spectroscopy (EELS), energy dispersion (EDS) and filtered imaging (EFTEM). Thus, thanks to these technological advances, it is possible to access chemical composition and structural information at atomistic scales and have greatly contributed to increasing the knowledge of the structural-function-properties of inorganic materials. While these high-resolution and chemical imaging approaches are widely applied to the field of material physics, they remain poorly transposed to biological systems, for several reasons: the need for advanced specific instrumentation (high resolution electronic microscopes with analytical configuration) ii) low concentration of elements to be detected, iii) fragility of biological samples subject to electron beam stresses, and finally iv) stability of the elements to be detected during sample preparation processes. Despite these significant technical challenges, the use of chemical imaging modes in biology remains a strong asset in the localisation of identification and visualisation of cellular structures and molecular assemblies, which are key points of many biological questions. By exploiting the different physical properties related to interactions between the electron beam and biological matter, electron microscopy with analytical configurations (META) remains a complementary and unavoidable approach to biochemical, molecular and chemical analyses; they optimise morphological studies at subcellular scales, establish chemical mappings of endogenous or exogenous elements, and thus enhance the contrast of imaging. A true link to the understanding of functional structure relationships, META is an indispensable tool for physio/pathological and toxicological studies. In this context, where chemical imaging remains a strong and innovative asset for biology, the CellSTEM project proposes the implementation of chemical imaging approaches in transmission electron microscopy through scanning modes (STEM), energy loss spectroscopy (EELS/EFTEM) and dispersive energy (EDS) to address as application components an understanding of the cellular mechanisms that lead to the development of cardiovascular and pulmonary pathologies in a dual physiopathological and environmental context. With more than 17,5 million deaths/year, cardiovascular disease is the world’s leading cause of death (WHO, 2012) with 4 out of 5 deaths from myocardial infarction. Encouraged by behavioural risks (smoking, poor diet obesity, installed diseases) they are increased by environmental factors (air pollution, noise stress and constitute a major health concern by the public authorities. The CellSTEM project based on META inputs will focus on assessing ultrastructural changes 1) endothelial and cardiac cells in different pathological contexts, and 2) endothelial and lung cells in a context of exposure to environmental stress. (English) / rank | |||||||||||||||
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| Property / summary: In the field of material physics transmission electron microscopy, has undergone rapid developments in terms of high resolution and spatially resolved spectroscopy for about ten years. The current electron microscopes are equipped with probe aberration correctors, and efficient spectrometers that allow the use of high resolution scanning modes (STEM), energy loss spectroscopy (EELS), energy dispersion (EDS) and filtered imaging (EFTEM). Thus, thanks to these technological advances, it is possible to access chemical composition and structural information at atomistic scales and have greatly contributed to increasing the knowledge of the structural-function-properties of inorganic materials. While these high-resolution and chemical imaging approaches are widely applied to the field of material physics, they remain poorly transposed to biological systems, for several reasons: the need for advanced specific instrumentation (high resolution electronic microscopes with analytical configuration) ii) low concentration of elements to be detected, iii) fragility of biological samples subject to electron beam stresses, and finally iv) stability of the elements to be detected during sample preparation processes. Despite these significant technical challenges, the use of chemical imaging modes in biology remains a strong asset in the localisation of identification and visualisation of cellular structures and molecular assemblies, which are key points of many biological questions. By exploiting the different physical properties related to interactions between the electron beam and biological matter, electron microscopy with analytical configurations (META) remains a complementary and unavoidable approach to biochemical, molecular and chemical analyses; they optimise morphological studies at subcellular scales, establish chemical mappings of endogenous or exogenous elements, and thus enhance the contrast of imaging. A true link to the understanding of functional structure relationships, META is an indispensable tool for physio/pathological and toxicological studies. In this context, where chemical imaging remains a strong and innovative asset for biology, the CellSTEM project proposes the implementation of chemical imaging approaches in transmission electron microscopy through scanning modes (STEM), energy loss spectroscopy (EELS/EFTEM) and dispersive energy (EDS) to address as application components an understanding of the cellular mechanisms that lead to the development of cardiovascular and pulmonary pathologies in a dual physiopathological and environmental context. With more than 17,5 million deaths/year, cardiovascular disease is the world’s leading cause of death (WHO, 2012) with 4 out of 5 deaths from myocardial infarction. Encouraged by behavioural risks (smoking, poor diet obesity, installed diseases) they are increased by environmental factors (air pollution, noise stress and constitute a major health concern by the public authorities. The CellSTEM project based on META inputs will focus on assessing ultrastructural changes 1) endothelial and cardiac cells in different pathological contexts, and 2) endothelial and lung cells in a context of exposure to environmental stress. (English) / qualifier | |||||||||||||||
point in time: 18 November 2021
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Revision as of 16:50, 18 November 2021
Project Q3681383 in France
| Language | Label | Description | Also known as |
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| English | No label defined |
Project Q3681383 in France |
Statements
378,000.00 Euro
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756,000.0 Euro
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50.0 percent
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1 January 2022
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UNIVERSITE DE ROUEN-NORMANDIE
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76821
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Dans le domaine de la physique des matériaux la microscopie électronique à transmission, a connu depuis une dizaine d'années, de rapides développements en termes de haute résolution et de spectroscopies résolues spatialement. Les microscopes électroniques actuels sont équipés de correcteurs d'aberration sur la sonde, et de spectromètres performants qui permettent l'utilisation des modes balayages pour la haute résolution (STEM), des spectroscopies de perte d'énergie (EELS), de dispersion d'énergie (EDS) et d'imagerie filtrée (EFTEM). Ainsi grâce à ces avancées techniques, il est possible d'accéder à des informations de composition chimique et de structure aux échelles atomistiques et ont fortement contribué à accroitre les connaissances des liens structures-fonctions-propriétés de matériaux inorganiques. Si ces approches de haute résolution et d'imagerie chimique sont largement appliquées au domaine de la physique des matériaux, elles restent peu transposées aux systèmes biologiques, pour plusieurs raisons : i) la nécessité d'avoir une instrumentation spécifique de pointe (microscopes électroniques haute résolution à configuration analytique) ii) la faible concentration des éléments à détecter, iii) la fragilité des échantillons biologiques soumis aux contraintes du faisceau d'électrons et enfin iv) la stabilité des éléments à détecter durant les processus de préparation des échantillons. En dépit de ces challenges techniques de taille, l'utilisation des modes d'imagerie chimique en biologie reste un atout fort dans la localisation détection identification et visualisation de structures cellulaires et assemblages moléculaires, qui sont des points clés de nombreuses questions biologiques. En exploitant les différentes propriétés physiques liées aux interactions entre le faisceau d'électrons et la matière biologique, la microscopie électronique en transmission à configurations analytique (META) reste une approche complémentaire et incontournable aux analyses biochimiques, moléculaires et chimiques ; elles permettent d'optimiser les études morphologiques aux échelles subcellulaires, d'établir des cartographies chimiques d'éléments endogènes ou exogènes, de renforcer le contraste donc l'imagerie. Véritable maillon pour la compréhension des relations structure fonction, la META est un outil indispensable aux études physio/pathologiques et toxicologiques. Dans ce contexte où l'imagerie chimique reste un atout fort et novateur pour la biologie, le projet CellSTEM propose la mise en oeuvre des approches d'imagerie chimique en microscopie électronique en transmission à travers les modes de balayage (STEM), les spectroscopies de perte d'énergie (EELS/EFTEM) et d'énergie dispersive (EDS) afin d'adresser comme volets applicatifs, la compréhension des mécanismes cellulaires qui conduisent au développement de pathologies cardiovasculaires et pulmonaires dans un contexte double physiopathologique d'une part et environnemental d'autre part. Avec plus de 17.5 millions de morts /an, les maladies cardio-vasculaires sont la première cause de mortalité dans le monde (WHO, 2012) avec 4 décès sur 5 par infarctus du myocarde. Favorisées par des risques comportementaux (tabagisme, mauvaise alimentation obésité, maladies installées) elles sont accrues par les facteurs environnementaux (pollution atmosphérique, stress sonore et constituent une préoccupation sanitaire majeure par les pouvoirs publics. Le projet CellSTEM basé sur les apports de la META s'attachera, à évaluer des modifications ultrastructurales 1) de cellules endothéliales et cardiaques dans différents contextes pathologiques et 2) de cellules endothéliales et pulmonaires dans un contexte d'exposition à des stress environnementaux. (French)
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In the field of material physics transmission electron microscopy, has undergone rapid developments in terms of high resolution and spatially resolved spectroscopy for about ten years. The current electron microscopes are equipped with probe aberration correctors, and efficient spectrometers that allow the use of high resolution scanning modes (STEM), energy loss spectroscopy (EELS), energy dispersion (EDS) and filtered imaging (EFTEM). Thus, thanks to these technological advances, it is possible to access chemical composition and structural information at atomistic scales and have greatly contributed to increasing the knowledge of the structural-function-properties of inorganic materials. While these high-resolution and chemical imaging approaches are widely applied to the field of material physics, they remain poorly transposed to biological systems, for several reasons: the need for advanced specific instrumentation (high resolution electronic microscopes with analytical configuration) ii) low concentration of elements to be detected, iii) fragility of biological samples subject to electron beam stresses, and finally iv) stability of the elements to be detected during sample preparation processes. Despite these significant technical challenges, the use of chemical imaging modes in biology remains a strong asset in the localisation of identification and visualisation of cellular structures and molecular assemblies, which are key points of many biological questions. By exploiting the different physical properties related to interactions between the electron beam and biological matter, electron microscopy with analytical configurations (META) remains a complementary and unavoidable approach to biochemical, molecular and chemical analyses; they optimise morphological studies at subcellular scales, establish chemical mappings of endogenous or exogenous elements, and thus enhance the contrast of imaging. A true link to the understanding of functional structure relationships, META is an indispensable tool for physio/pathological and toxicological studies. In this context, where chemical imaging remains a strong and innovative asset for biology, the CellSTEM project proposes the implementation of chemical imaging approaches in transmission electron microscopy through scanning modes (STEM), energy loss spectroscopy (EELS/EFTEM) and dispersive energy (EDS) to address as application components an understanding of the cellular mechanisms that lead to the development of cardiovascular and pulmonary pathologies in a dual physiopathological and environmental context. With more than 17,5 million deaths/year, cardiovascular disease is the world’s leading cause of death (WHO, 2012) with 4 out of 5 deaths from myocardial infarction. Encouraged by behavioural risks (smoking, poor diet obesity, installed diseases) they are increased by environmental factors (air pollution, noise stress and constitute a major health concern by the public authorities. The CellSTEM project based on META inputs will focus on assessing ultrastructural changes 1) endothelial and cardiac cells in different pathological contexts, and 2) endothelial and lung cells in a context of exposure to environmental stress. (English)
18 November 2021
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Identifiers
18P02425
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