EFFECT OF EMERGING CONTAMINANTS IN MALE GERMLINE CELLS: PATERNAL CONTRIBUTION TO TRANSGENERATIONAL DEVELOPMENT AND INHERITANCE (Q3204189): Difference between revisions
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(Created claim: summary (P836): SUB-PROJECT 3 WILL AIM TO ANALYSE THE EFFECTS OF BISPHENOL A, EE2 AND CIMETIDINE ON MALE GERMLINE CELLS, USING ZEBRAFISH AS A MODEL, SINCE IT ALLOWS UNVIABLE EXPOSURE PROTOCOLS IN COMMERCIAL SPECIES. PATERNAL CONTRIBUTION IS CRUCIAL TO THE CONTROL OF EARLY EMBRYONIC DEVELOPMENT THROUGH THE EXPRESSION OF CERTAIN GENES, THE TRANSCRIPTION OF CERTAIN MSRNAS AND THE MAINTENANCE OF A CORRECT EPIGENETIC PATTERN, WHICH IS REPRODUCED IN THE EMBRYO 24HPF....) |
(Changed label, description and/or aliases in en: translated_label) |
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EFFECT OF EMERGING CONTAMINANTS IN MALE GERMLINE CELLS: PATERNAL CONTRIBUTION TO TRANSGENERATIONAL DEVELOPMENT AND INHERITANCE | |||
Revision as of 00:42, 13 October 2021
Project Q3204189 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | EFFECT OF EMERGING CONTAMINANTS IN MALE GERMLINE CELLS: PATERNAL CONTRIBUTION TO TRANSGENERATIONAL DEVELOPMENT AND INHERITANCE |
Project Q3204189 in Spain |
Statements
111,925.0 Euro
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223,850.0 Euro
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50.0 percent
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1 January 2015
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31 July 2018
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UNIVERSIDAD DE LEON
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42°38'2.94"N, 5°58'17.11"W
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24089
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EL SUBPROYECTO 3 TENDRA COMO OBJETIVO ANALIZAR LOS EFECTOS DEL BISFENOL A, EE2 Y CIMETIDINA EN LAS CELULAS DE LA LINEA GERMINAL MASCULINA, UTILIZANDO COMO MODELO EL PEZ CEBRA, YA QUE PERMITE LA REALIZACION DE PROTOCOLOS DE EXPOSICION INVIABLES EN ESPECIES COMERCIALES. LA CONTRIBUCION PATERNA ES CRUCIAL PARA EL CONTROL DEL DESARROLLO EMBRIONARIO TEMPRANO A TRAVES DE LA EXPRESION DE DETERMINADOS GENES, LA TRANSCRIPCION DE DETERMINADOS ARNMS Y EL MANTENIMIENTO DE UN CORRECTO PATRON EPIGENETICO, QUE ES REPRODUCIDO EN EL EMBRION 24HPF. LOS CONTAMINANTES EMERGENTES PUEDEN MODIFICAR DICHA INFORMACION Y AFECTAR, COMO YA SE HA COMPROBADO, AL DESARROLLO DE LA F1. NUESTRO ESTUDIO ANALIZARA EN ESPERMATOZOIDES DE ANIMALES EXPUESTOS A LOS TOXICOS DURANTE LA VIDA EMBRIONARIA O ADULTA, LOS POTENCIALES DAÑOS EN EL GENOMA, TRANSCRIPTOMA Y EPIGENOMA, IDENTIFICANDO GENES CANDIDATOS DE DAÑO GENOTOXICO Y EPIGENOTOXICO. PARA ELLO SE EMPLEARAN TECNICAS PUESTAS A PUNTO EN NUESTRO LABORATORIO, COLABORANDO CON OTROS EQUIPOS PARA LA REALIZACION DEL RRBS. ESTOS ESTUDIOS NOS PERMITIRAN IDENTIFICAR LAS FASES MAS SENSIBLES Y LOS ELEMENTOS POTENCIALMENTE AFECTADOS POR LOS AGENTES TOXICOS, IDENTIFICANDO LOS GENES O PROMOTORES MAS VULNERABLES Y ESTABLECIENDO UN PANEL DE INDICADORES DE DAÑO EN LA LINEA GERMINAL. _x000D_ EN LA DESCENDENCIA DE LOS PROGENITORES EXPUESTOS SE EVALUARA LA MORFOGENESIS, CON ESPECIAL ATENCION A LA FORMACION DEL CORAZON, LA CAPACIDAD REPRODUCTORA, RESPUESTA INMUNE, CAPACIDAD DE REGENERACION DE TEJIDOS Y TASA DE ENVEJECIMIENTO, UTILIZANDO ESTUDIOS MORFOLOGICOS, FUNCIONALES Y DE EXPRESION GENICA. LA POSIBLE TRANSMISION DE LA INFORMACION PATERNA A LAS CELULAS DE LA LINEA GERMINAL DE LA F1, QUE PROVOCARIAN UNA HERENCIA TRANSGENERACIONAL A LA F2 SE ANALIZARA MEDIANTE EL ANALISIS DE LAS CELULAS DE LA LINEA GERMINAL DE LOS MARCADORES DE TOXICIDAD PREVIAMENTE DEFINIDOS. EN SU CASO, TAMBIEN SERA EVALUADO EL DESARROLLO DE LA F2 UTILIZANDO LOS MISMOS PARAMETROS QUE CON LA F1. SE EVALUARA ADEMAS EL POSIBLE RESCATE NUTRICIONAL DE LOS DAÑOS EPIGENOTOXICOS, QUE PODRIAN DEBERSE A UNA MENOR ACTIVIDAD DE LAS METIL TRANSFERASAS O A UNA MENOR DISPONIBILIDAD DE GRUPOS METILO, MEDIANTE LA ADMINISTRACION DE DIETAS RICAS EN FOLATOS. LA INFLUENCIA DEL MICROAMBIENTE SOMATICO EN LA RECUPERACION DE LOS EFECTOS TOXICOS SOBRE LAS CELULAS DE LA LINEA GERMINAL SERA ESTUDIADA MEDIANTE EL TRASPLANTE DE CELULAS GERMINALES ENTRE ANIMALES EXPUESTOS Y ANIMALES CONTROL PREVIAMENTE ESTERILIZADOS POR ELIMINACION DE SUS CELULAS GERMINALES CON MORFOLINO DND._x000D_ PARA LA REALIZACION DEL ESTUDIO SE UTILIZARAN LINEAS WILD TYPE (AB) Y TRANSGENICAS (VASA::EGFP), QUE PERMITIRAN EL AISLAMIENTO, TRANSPLANTE Y ESTUDIO DE LA EVOLUCION DE LAS CELULAS DE LA LINEA GERMINAL (Spanish)
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SUB-PROJECT 3 WILL AIM TO ANALYSE THE EFFECTS OF BISPHENOL A, EE2 AND CIMETIDINE ON MALE GERMLINE CELLS, USING ZEBRAFISH AS A MODEL, SINCE IT ALLOWS UNVIABLE EXPOSURE PROTOCOLS IN COMMERCIAL SPECIES. PATERNAL CONTRIBUTION IS CRUCIAL TO THE CONTROL OF EARLY EMBRYONIC DEVELOPMENT THROUGH THE EXPRESSION OF CERTAIN GENES, THE TRANSCRIPTION OF CERTAIN MSRNAS AND THE MAINTENANCE OF A CORRECT EPIGENETIC PATTERN, WHICH IS REPRODUCED IN THE EMBRYO 24HPF. EMERGING POLLUTANTS CAN MODIFY THIS INFORMATION AND AFFECT, AS HAS ALREADY BEEN SHOWN, THE DEVELOPMENT OF F1. OUR STUDY WILL ANALYSE POTENTIAL DAMAGE TO THE GENOME, TRANSCRIPTOME, AND EPIGENOME IN ANIMALS EXPOSED TO TOXICS DURING EMBRYONIC OR ADULT LIFE, IDENTIFYING GENES FOR GENOTOXIC AND EPIGENOTOXIC DAMAGE. FOR THIS PURPOSE TECHNIQUES WILL BE USED DEVELOPED IN OUR LABORATORY, COLLABORATING WITH OTHER TEAMS FOR THE REALISATION OF THE RRBS. THESE STUDIES WILL ALLOW US TO IDENTIFY THE MOST SENSITIVE PHASES AND THE ELEMENTS POTENTIALLY AFFECTED BY TOXIC AGENTS, IDENTIFYING THE MOST VULNERABLE GENES OR PROMOTERS AND ESTABLISHING A PANEL OF DAMAGE INDICATORS IN THE GERM LINE. _x000D_ in the DESCENDENCE OF EXPUEST PROGENITORS will be evaluated morphogenesis, with special attention to HEART FORMATION, reproductive capacity, IMMUNE RESPERTY, TEJID REGENERATION CAPACITY AND Aging Tax, using MORFOLOGICAL, FUNCIONAL and GENIC EXPRESSION STUDIES. THE POSSIBLE TRANSMISSION OF PATERNAL INFORMATION TO THE CELLS OF THE GERMINAL LINE OF THE F1, WHICH PROVOCARIAN A TRANSGENERATIONAL INHERITANCE TO F2 WAS ANALYSED BY ANALYSING THE GERMLINE CELLS OF THE PREVIOUSLY DEFINED TOXICITY MARKERS. IF APPLICABLE, THE DEVELOPMENT OF F2 WILL ALSO BE EVALUATED USING THE SAME PARAMETERS AS WITH F1. THE POSSIBLE NUTRITIONAL RESCUE OF EPIGENOTOXIC DAMAGE, WHICH MAY BE DUE TO LOWER METHYL TRANSFERASE ACTIVITY OR REDUCED AVAILABILITY OF METHYL GROUPS, WILL ALSO BE EVALUATED THROUGH THE ADMINISTRATION OF FOLATE-RICH DIETS. The INFLUENCE of the somatic microenvironment IN THE RECOVERY OF TOXIC EFFECTS ON THE GERMINAL LINE CELLS IS STUDIATED FOR THE TRANSPLANT OF GERMINAL CELULS EXPUESED AND ANIMAL CONTROL PREVIENTLY STERILISED by ELIMINATION OF YOUR GERMINAL CELLS WITH morfolin DND._x000D_ for the realisation of the state, WILD TYPE (AB) and TRANSGENICAS (VASA:EGFP) will be used, which will allow the isolation, transPLANT AND STUDY OF THE EVOLUTION OF THE GERMINAL CELLS (English)
13 October 2021
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León
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Identifiers
AGL2014-53167-C3-3-R
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