Development of a diagnostic system for patients with pancreatic adenocarcinoma based on previous clinical results of peripheral blood differential gene expression (Q3176398): Difference between revisions

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(‎Removed claim: summary (P836): Pancreatic ductal adenocarcinoma (PDAC) is the fourth cause of death from cancer. The lack of sensitive and specific biomarkers for early detection is one of the main constraints. In this context, the determination of the peripheral blood gene expression profile of patients has been shown to be useful as a less invasive diagnostic tool than biopsy. On the other hand, mass sequencing technology is allowing thousands of genes to be analysed simu...)
Property / summary
Pancreatic ductal adenocarcinoma (PDAC) is the fourth cause of death from cancer. The lack of sensitive and specific biomarkers for early detection is one of the main constraints. In this context, the determination of the peripheral blood gene expression profile of patients has been shown to be useful as a less invasive diagnostic tool than biopsy. On the other hand, mass sequencing technology is allowing thousands of genes to be analysed simultaneously in a fast, reproducible and less expensive way. Our previous clinical results, which have an international patent (WO 2014/076342), obtained by microarrays, have allowed us to identify a differential gene expression profile based on four genes that, we have shown, are biomarkers of differentials of patients with PDAC versus healthy subjects. The objectives of this project are to: 1. Validate the sensitivity and specificity of the differential expression of our biomarkers already identified in peripheral blood using the innovative RNAseq technology; 2. Determine the existence of new potential biomarkers following the RNAseq study; 3. Develop a diagnostic kit for pancreatic adenocarcinoma based on this panel of peripheral blood biomarkers, which can be used in normal clinical practice. The following methodology shall be used for this purpose: 1. Obtaining total peripheral blood RNA from healthy subjects and patients with PDAC after treatment; 2. Determination of gene expression profile in peripheral blood samples using RNAseq; 3. Data processing using specific software; 4. Validation of results obtained by quantitative PCR in real time; 5. Development of diagnostic kit for commercialisation and exploitation through the company GENÓMICA S.A.U. (English)
 
Property / summary: Pancreatic ductal adenocarcinoma (PDAC) is the fourth cause of death from cancer. The lack of sensitive and specific biomarkers for early detection is one of the main constraints. In this context, the determination of the peripheral blood gene expression profile of patients has been shown to be useful as a less invasive diagnostic tool than biopsy. On the other hand, mass sequencing technology is allowing thousands of genes to be analysed simultaneously in a fast, reproducible and less expensive way. Our previous clinical results, which have an international patent (WO 2014/076342), obtained by microarrays, have allowed us to identify a differential gene expression profile based on four genes that, we have shown, are biomarkers of differentials of patients with PDAC versus healthy subjects. The objectives of this project are to: 1. Validate the sensitivity and specificity of the differential expression of our biomarkers already identified in peripheral blood using the innovative RNAseq technology; 2. Determine the existence of new potential biomarkers following the RNAseq study; 3. Develop a diagnostic kit for pancreatic adenocarcinoma based on this panel of peripheral blood biomarkers, which can be used in normal clinical practice. The following methodology shall be used for this purpose: 1. Obtaining total peripheral blood RNA from healthy subjects and patients with PDAC after treatment; 2. Determination of gene expression profile in peripheral blood samples using RNAseq; 3. Data processing using specific software; 4. Validation of results obtained by quantitative PCR in real time; 5. Development of diagnostic kit for commercialisation and exploitation through the company GENÓMICA S.A.U. (English) / rank
Normal rank
 
Property / summary: Pancreatic ductal adenocarcinoma (PDAC) is the fourth cause of death from cancer. The lack of sensitive and specific biomarkers for early detection is one of the main constraints. In this context, the determination of the peripheral blood gene expression profile of patients has been shown to be useful as a less invasive diagnostic tool than biopsy. On the other hand, mass sequencing technology is allowing thousands of genes to be analysed simultaneously in a fast, reproducible and less expensive way. Our previous clinical results, which have an international patent (WO 2014/076342), obtained by microarrays, have allowed us to identify a differential gene expression profile based on four genes that, we have shown, are biomarkers of differentials of patients with PDAC versus healthy subjects. The objectives of this project are to: 1. Validate the sensitivity and specificity of the differential expression of our biomarkers already identified in peripheral blood using the innovative RNAseq technology; 2. Determine the existence of new potential biomarkers following the RNAseq study; 3. Develop a diagnostic kit for pancreatic adenocarcinoma based on this panel of peripheral blood biomarkers, which can be used in normal clinical practice. The following methodology shall be used for this purpose: 1. Obtaining total peripheral blood RNA from healthy subjects and patients with PDAC after treatment; 2. Determination of gene expression profile in peripheral blood samples using RNAseq; 3. Data processing using specific software; 4. Validation of results obtained by quantitative PCR in real time; 5. Development of diagnostic kit for commercialisation and exploitation through the company GENÓMICA S.A.U. (English) / qualifier
point in time: 12 October 2021
Timestamp+2021-10-12T00:00:00Z
Timezone+00:00
CalendarGregorian
Precision1 day
Before0
After0
 

Revision as of 18:47, 12 October 2021

Project Q3176398 in Spain
Language Label Description Also known as
English
Development of a diagnostic system for patients with pancreatic adenocarcinoma based on previous clinical results of peripheral blood differential gene expression
Project Q3176398 in Spain

    Statements

    country
    Spain
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    EU contribution
    24,800.0 Euro
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    budget
    31,000.0 Euro
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    co-financing rate
    80.0 percent
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    start time
    1 January 2016
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    end time
    16 December 2017
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    beneficiary name (string)
    UNIVERSIDAD DE JAEN
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    coordinate location

    37°57'20.63"N, 3°29'31.42"W
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    postal code
    23050
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    summary
    El adenocarcinoma ductal pancreático (PDAC), es la cuarta causa de muerte por cáncer. La falta de biomarcadores sensibles y específicos para su detección temprana constituye una de las principales limitaciones. En este contexto, la determinación del perfil de expresión génica en sangre periférica de pacientes ha demostrado ser útil como herramienta diagnóstica menos invasiva que la biopsia. Por otro lado, la tecnología de secuenciación masiva está permitiendo analizar simultáneamente miles de genes de manera rápida, reproducible y cada vez menos costosa. Nuestros resultados clínicos previos, que poseen patente internacional (WO 2014/076342), obtenidos mediante microarrays, nos han permitido identificar un perfil de expresión génica diferencial basado en cuatro genes que, hemos demostrado, son biomarcadores de diferenciales de pacientes con PDAC frente a sujetos sanos. Los objetivos del presente proyecto son: 1. Validar la sensibilidad y especificidad de la expresión diferencial de nuestros biomarcadores ya identificados en sangre periférica usando la novedosa tecnología de RNAseq; 2. Determinar la existencia de nuevos posibles biomarcadores tras el estudio de RNAseq; 3. Desarrollar un kit diagnóstico para el adenocarcinoma de páncreas basado en dicho panel de biomarcadores de sangre periférica, que pueda ser usado en la práctica clínica habitual. Para ello se usará la siguiente metodología: 1. Obtención del ARN total de sangre periférica de sujetos sanos y pacientes con PDAC previo tratamiento; 2. Determinación del perfil de expresión génica en muestras de sangre periférica mediante RNAseq; 3. Procesamiento de datos mediante software específico; 4. Validación de resultados obtenidos por PCR cuantitativa a tiempo real; 5. Desarrollo de kit diagnóstico para su comercialización y explotación a través de la empresa GENÓMICA S.A.U. (Spanish)
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    location (string)
    Jaén
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    Identifiers

    Spanish Kohesio ID
    DTS15_00201
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