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(Created claim: summary (P836): THE SET OF MICROORGANISMS THAT HABITUALLY INHABIT THE HUMAN BODY, KNOWN AS “MICROBIOMA”, PERFORMS A SERIES OF BENEFICIAL FUNCTIONS FOR OUR HEALTH, SOME OF WHICH ARE BEGINNING TO UNDERSTAND AND CHARACTERISE. IN THIS PROJECT, WE INTEND TO USE THE TECHNIQUES OF MASS SEQUENCING, METAGENOMICA AND MOLECULAR MICROBIOLOGY TO CHARACTERISE THE ORAL MICROBIOTA IN CONDITIONS OF HEALTH AND DISEASE, AND USE THIS INFORMATION FOR THE DEVELOPMENT OF DIAGNOSTIC A...) |
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THE SET OF MICROORGANISMS THAT HABITUALLY INHABIT THE HUMAN BODY, KNOWN AS “MICROBIOMA”, PERFORMS A SERIES OF BENEFICIAL FUNCTIONS FOR OUR HEALTH, SOME OF WHICH ARE BEGINNING TO UNDERSTAND AND CHARACTERISE. IN THIS PROJECT, WE INTEND TO USE THE TECHNIQUES OF MASS SEQUENCING, METAGENOMICA AND MOLECULAR MICROBIOLOGY TO CHARACTERISE THE ORAL MICROBIOTA IN CONDITIONS OF HEALTH AND DISEASE, AND USE THIS INFORMATION FOR THE DEVELOPMENT OF DIAGNOSTIC AND PREVENTIVE STRATEGIES. THE PROJECT CONSISTS OF THREE MAIN WORK PACKAGES: 1) ORAL MICROBIOTA DEVELOPMENT FROM BIRTH TO 7 YEARS OF AGE IN CHILDREN WHO SUFFER AND DO NOT HAVE CAVITIES. SAMPLES AT 3, 6, 12, 24 AND 84 MONTHS OF AGE WILL BE USED IN A COHORT OF SWEDISH CHILDREN, HALF OF WHOM DEVELOP CARIES, TO STUDY THE DEVELOPMENT OF THE MICROBIOTA ASSOCIATED WITH GOOD ORAL HEALTH AND THAT CARIOGENICA, USING ILLUMINA SEQUENCING OF THE RIBOSOMAL GENE, IN ADDITION TO MEASURING BACTERIAL AND FUNGIC LOAD OVER TIME AND LEVELS OF IMMUNOGLUBULIN TO SALIVA. 2) CHARACTERISATION OF BENEFICIAL FUNCTIONS BY THE ORAL MICROBIOME, IN PARTICULAR THE TAMPON EFFECT ON PH, AND THE REDUCTION OF NITRATE. THE BUFFER EFFECT OF DENTAL PLAQUE IS DUE TO METABOLIC PATHWAYS SUCH AS UREASE OR ARGINOLITICA ACTIVITY, WHICH LEAD TO THE PRODUCTION OF AMMONIUM, WHICH NEUTRALISES THE ACIDS CAUSING THE DEMINERALISATION OF THE ENAMEL. IN ADDITION, PH NOT ONLY AFFECTS THE RISK OF DENTAL CARIES, BUT ALSO THE DIGESTION OF CARBOHYDRATES, DUE TO THE IMPORTANCE OF PREDIGESTION THAT TAKES PLACE IN THE MOUTH, WHERE THE ALPHA-AMYLASE RESPONSIBLE FOR THIS PROCESS HAS AN OPTIMAL PH OF 6.7 TO 7. THE NITRATE REDUCTASE ACTIVITY OF ORAL BACTERIA IS ALSO FUNDAMENTAL FOR THE PRODUCTION OF NITRIC OXIDE, WHICH HAS A CLEAR SYSTEMIC EFFECT ON CARDIOVASCULAR HEALTH, AMONG OTHER IMPORTANT PHYSIOLOGICAL EFFECTS; 3) CHARACTERISATION OF THE MICROBIOTA AND THE METABOLIC PATHWAYS ASSOCIATED WITH HALITOSIS, BY MASS SEQUENCING OF METATRANSCRIPTOMA. THIS DISEASE, WHICH DOES NOT YET HAVE EFFECTIVE THERAPEUTIC MEASURES, IS CAUSED BY THE PRODUCTION OF VOLATILE COMPOUNDS BY ORAL BACTERIA, AND THE ETHIOLOGIC AGENTS OF IT ARE NOT ELUCIDATED, ALTHOUGH IT IS KNOWN TO HAVE A POLYMICROBIAL ORIGIN. By means of the bio-informatics of GENES EXPRESED IN HEALTH AND HALITOSIS CONDITIONS, we may identify the active MICROORGANISMS AND EXPRESSED GENES RESPONSIBLE OF PATOGENICA ACTIVITY._x000D_ THIS INFORMATION WITH TWO FINES APPLICED: _x000D_ 1) THE DEVELOPMENT OF MOLECULAR DIAGNOSTIC KITS determining the TAMPON capacity of the platform (UREASA AND ARGININOLITIC ACTIVITY) and NITRIT PRODUCTION CAPACITY OF ORAL MICROBIOMA (REDUCTOR NITRATE ACTIVITY); THESE TESTS WERE USED TO RELATE TAMPON CAPACITY TO THE RISK OF CARIES AND DIGESTIVE PROBLEMS, AS WELL AS TO RELATE THE PRODUCTION CAPACITY OF NITRITE TO BLOOD PRESSURE, WHICH COULD EXPLAIN THE EPIDEMIOLOGICAL CORRELATION BETWEEN ORAL DISEASES AND CARDIOVASCULAR RISK. _x000D_ 2) SELECTION OF probiotic SPECIES, WHAT WHAT IS Isolated OF INDIVIDUUS WITHOUT THE PATOLOGY OF CORRESPONDIENT IN THE NICHOUSE WHERE WHERE YOUR ACCOUNT MUST BE YOUR ACCOUNT, AND YOU CAN BE USED AS PREVENTIVE STRATEGIES FROM THE CARIES, THE MALA ABSORCTION OF CARBOHYDRATS, HALITOSIS AND CARDIOVASCULAR DISEASES. (English) | |||||||||||||||
| Property / summary: THE SET OF MICROORGANISMS THAT HABITUALLY INHABIT THE HUMAN BODY, KNOWN AS “MICROBIOMA”, PERFORMS A SERIES OF BENEFICIAL FUNCTIONS FOR OUR HEALTH, SOME OF WHICH ARE BEGINNING TO UNDERSTAND AND CHARACTERISE. IN THIS PROJECT, WE INTEND TO USE THE TECHNIQUES OF MASS SEQUENCING, METAGENOMICA AND MOLECULAR MICROBIOLOGY TO CHARACTERISE THE ORAL MICROBIOTA IN CONDITIONS OF HEALTH AND DISEASE, AND USE THIS INFORMATION FOR THE DEVELOPMENT OF DIAGNOSTIC AND PREVENTIVE STRATEGIES. THE PROJECT CONSISTS OF THREE MAIN WORK PACKAGES: 1) ORAL MICROBIOTA DEVELOPMENT FROM BIRTH TO 7 YEARS OF AGE IN CHILDREN WHO SUFFER AND DO NOT HAVE CAVITIES. SAMPLES AT 3, 6, 12, 24 AND 84 MONTHS OF AGE WILL BE USED IN A COHORT OF SWEDISH CHILDREN, HALF OF WHOM DEVELOP CARIES, TO STUDY THE DEVELOPMENT OF THE MICROBIOTA ASSOCIATED WITH GOOD ORAL HEALTH AND THAT CARIOGENICA, USING ILLUMINA SEQUENCING OF THE RIBOSOMAL GENE, IN ADDITION TO MEASURING BACTERIAL AND FUNGIC LOAD OVER TIME AND LEVELS OF IMMUNOGLUBULIN TO SALIVA. 2) CHARACTERISATION OF BENEFICIAL FUNCTIONS BY THE ORAL MICROBIOME, IN PARTICULAR THE TAMPON EFFECT ON PH, AND THE REDUCTION OF NITRATE. THE BUFFER EFFECT OF DENTAL PLAQUE IS DUE TO METABOLIC PATHWAYS SUCH AS UREASE OR ARGINOLITICA ACTIVITY, WHICH LEAD TO THE PRODUCTION OF AMMONIUM, WHICH NEUTRALISES THE ACIDS CAUSING THE DEMINERALISATION OF THE ENAMEL. IN ADDITION, PH NOT ONLY AFFECTS THE RISK OF DENTAL CARIES, BUT ALSO THE DIGESTION OF CARBOHYDRATES, DUE TO THE IMPORTANCE OF PREDIGESTION THAT TAKES PLACE IN THE MOUTH, WHERE THE ALPHA-AMYLASE RESPONSIBLE FOR THIS PROCESS HAS AN OPTIMAL PH OF 6.7 TO 7. THE NITRATE REDUCTASE ACTIVITY OF ORAL BACTERIA IS ALSO FUNDAMENTAL FOR THE PRODUCTION OF NITRIC OXIDE, WHICH HAS A CLEAR SYSTEMIC EFFECT ON CARDIOVASCULAR HEALTH, AMONG OTHER IMPORTANT PHYSIOLOGICAL EFFECTS; 3) CHARACTERISATION OF THE MICROBIOTA AND THE METABOLIC PATHWAYS ASSOCIATED WITH HALITOSIS, BY MASS SEQUENCING OF METATRANSCRIPTOMA. THIS DISEASE, WHICH DOES NOT YET HAVE EFFECTIVE THERAPEUTIC MEASURES, IS CAUSED BY THE PRODUCTION OF VOLATILE COMPOUNDS BY ORAL BACTERIA, AND THE ETHIOLOGIC AGENTS OF IT ARE NOT ELUCIDATED, ALTHOUGH IT IS KNOWN TO HAVE A POLYMICROBIAL ORIGIN. By means of the bio-informatics of GENES EXPRESED IN HEALTH AND HALITOSIS CONDITIONS, we may identify the active MICROORGANISMS AND EXPRESSED GENES RESPONSIBLE OF PATOGENICA ACTIVITY._x000D_ THIS INFORMATION WITH TWO FINES APPLICED: _x000D_ 1) THE DEVELOPMENT OF MOLECULAR DIAGNOSTIC KITS determining the TAMPON capacity of the platform (UREASA AND ARGININOLITIC ACTIVITY) and NITRIT PRODUCTION CAPACITY OF ORAL MICROBIOMA (REDUCTOR NITRATE ACTIVITY); THESE TESTS WERE USED TO RELATE TAMPON CAPACITY TO THE RISK OF CARIES AND DIGESTIVE PROBLEMS, AS WELL AS TO RELATE THE PRODUCTION CAPACITY OF NITRITE TO BLOOD PRESSURE, WHICH COULD EXPLAIN THE EPIDEMIOLOGICAL CORRELATION BETWEEN ORAL DISEASES AND CARDIOVASCULAR RISK. _x000D_ 2) SELECTION OF probiotic SPECIES, WHAT WHAT IS Isolated OF INDIVIDUUS WITHOUT THE PATOLOGY OF CORRESPONDIENT IN THE NICHOUSE WHERE WHERE YOUR ACCOUNT MUST BE YOUR ACCOUNT, AND YOU CAN BE USED AS PREVENTIVE STRATEGIES FROM THE CARIES, THE MALA ABSORCTION OF CARBOHYDRATS, HALITOSIS AND CARDIOVASCULAR DISEASES. (English) / rank | |||||||||||||||
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| Property / summary: THE SET OF MICROORGANISMS THAT HABITUALLY INHABIT THE HUMAN BODY, KNOWN AS “MICROBIOMA”, PERFORMS A SERIES OF BENEFICIAL FUNCTIONS FOR OUR HEALTH, SOME OF WHICH ARE BEGINNING TO UNDERSTAND AND CHARACTERISE. IN THIS PROJECT, WE INTEND TO USE THE TECHNIQUES OF MASS SEQUENCING, METAGENOMICA AND MOLECULAR MICROBIOLOGY TO CHARACTERISE THE ORAL MICROBIOTA IN CONDITIONS OF HEALTH AND DISEASE, AND USE THIS INFORMATION FOR THE DEVELOPMENT OF DIAGNOSTIC AND PREVENTIVE STRATEGIES. THE PROJECT CONSISTS OF THREE MAIN WORK PACKAGES: 1) ORAL MICROBIOTA DEVELOPMENT FROM BIRTH TO 7 YEARS OF AGE IN CHILDREN WHO SUFFER AND DO NOT HAVE CAVITIES. SAMPLES AT 3, 6, 12, 24 AND 84 MONTHS OF AGE WILL BE USED IN A COHORT OF SWEDISH CHILDREN, HALF OF WHOM DEVELOP CARIES, TO STUDY THE DEVELOPMENT OF THE MICROBIOTA ASSOCIATED WITH GOOD ORAL HEALTH AND THAT CARIOGENICA, USING ILLUMINA SEQUENCING OF THE RIBOSOMAL GENE, IN ADDITION TO MEASURING BACTERIAL AND FUNGIC LOAD OVER TIME AND LEVELS OF IMMUNOGLUBULIN TO SALIVA. 2) CHARACTERISATION OF BENEFICIAL FUNCTIONS BY THE ORAL MICROBIOME, IN PARTICULAR THE TAMPON EFFECT ON PH, AND THE REDUCTION OF NITRATE. THE BUFFER EFFECT OF DENTAL PLAQUE IS DUE TO METABOLIC PATHWAYS SUCH AS UREASE OR ARGINOLITICA ACTIVITY, WHICH LEAD TO THE PRODUCTION OF AMMONIUM, WHICH NEUTRALISES THE ACIDS CAUSING THE DEMINERALISATION OF THE ENAMEL. IN ADDITION, PH NOT ONLY AFFECTS THE RISK OF DENTAL CARIES, BUT ALSO THE DIGESTION OF CARBOHYDRATES, DUE TO THE IMPORTANCE OF PREDIGESTION THAT TAKES PLACE IN THE MOUTH, WHERE THE ALPHA-AMYLASE RESPONSIBLE FOR THIS PROCESS HAS AN OPTIMAL PH OF 6.7 TO 7. THE NITRATE REDUCTASE ACTIVITY OF ORAL BACTERIA IS ALSO FUNDAMENTAL FOR THE PRODUCTION OF NITRIC OXIDE, WHICH HAS A CLEAR SYSTEMIC EFFECT ON CARDIOVASCULAR HEALTH, AMONG OTHER IMPORTANT PHYSIOLOGICAL EFFECTS; 3) CHARACTERISATION OF THE MICROBIOTA AND THE METABOLIC PATHWAYS ASSOCIATED WITH HALITOSIS, BY MASS SEQUENCING OF METATRANSCRIPTOMA. THIS DISEASE, WHICH DOES NOT YET HAVE EFFECTIVE THERAPEUTIC MEASURES, IS CAUSED BY THE PRODUCTION OF VOLATILE COMPOUNDS BY ORAL BACTERIA, AND THE ETHIOLOGIC AGENTS OF IT ARE NOT ELUCIDATED, ALTHOUGH IT IS KNOWN TO HAVE A POLYMICROBIAL ORIGIN. By means of the bio-informatics of GENES EXPRESED IN HEALTH AND HALITOSIS CONDITIONS, we may identify the active MICROORGANISMS AND EXPRESSED GENES RESPONSIBLE OF PATOGENICA ACTIVITY._x000D_ THIS INFORMATION WITH TWO FINES APPLICED: _x000D_ 1) THE DEVELOPMENT OF MOLECULAR DIAGNOSTIC KITS determining the TAMPON capacity of the platform (UREASA AND ARGININOLITIC ACTIVITY) and NITRIT PRODUCTION CAPACITY OF ORAL MICROBIOMA (REDUCTOR NITRATE ACTIVITY); THESE TESTS WERE USED TO RELATE TAMPON CAPACITY TO THE RISK OF CARIES AND DIGESTIVE PROBLEMS, AS WELL AS TO RELATE THE PRODUCTION CAPACITY OF NITRITE TO BLOOD PRESSURE, WHICH COULD EXPLAIN THE EPIDEMIOLOGICAL CORRELATION BETWEEN ORAL DISEASES AND CARDIOVASCULAR RISK. _x000D_ 2) SELECTION OF probiotic SPECIES, WHAT WHAT IS Isolated OF INDIVIDUUS WITHOUT THE PATOLOGY OF CORRESPONDIENT IN THE NICHOUSE WHERE WHERE YOUR ACCOUNT MUST BE YOUR ACCOUNT, AND YOU CAN BE USED AS PREVENTIVE STRATEGIES FROM THE CARIES, THE MALA ABSORCTION OF CARBOHYDRATS, HALITOSIS AND CARDIOVASCULAR DISEASES. (English) / qualifier | |||||||||||||||
point in time: 12 October 2021
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Revision as of 15:32, 12 October 2021
Project Q3152561 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | No label defined |
Project Q3152561 in Spain |
Statements
102,850.0 Euro
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205,700.0 Euro
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50.0 percent
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1 January 2016
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31 March 2019
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FUNDACION PARA EL FOMENTO DE LA INV. SANITARIA Y BIOMEDICA DE LA COMUNIDAD VALENCIANA (FISABIO)
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39°28'10.96"N, 0°22'34.82"W
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46250
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EL CONJUNTO DE MICROORGANISMOS QUE HABITAN DE FORMA HABITUAL EL CUERPO HUMANO, CONOCIDO COMO ¿MICROBIOMA¿, REALIZA UNA SERIE DE FUNCIONES BENEFICIOSAS PARA NUESTRA SALUD, ALGUNAS DE LAS CUALES SE ESTAN EMPEZANDO A ENTENDER Y CARACTERIZAR. EN ESTE PROYECTO, PRETENDEMOS UTILIZAR LAS TECNICAS DE SECUENCIACION MASIVA, METAGENOMICA Y MICROBIOLOGIA MOLECULAR PARA CARACTERIZAR LA MICROBIOTA ORAL EN CONDICIONES DE SALUD Y ENFERMEDAD, Y UTILIZAR ESTA INFORMACION PARA EL DESARROLLO DE ESTRATEGIAS DIAGNOSTICAS Y PREVENTIVAS. EL PROYECTO CONSTA DE TRES PAQUETES DE TRABAJO PRINCIPALES: 1) DESARROLLO DE LA MICROBIOTA ORAL DESDE EL NACIMIENTO A LOS 7 AÑOS DE EDAD EN NIÑOS QUE PADECEN Y NO PADECEN CARIES. SE UTILIZARAN MUESTRAS A LOS 3, 6, 12, 24 Y 84 MESES DE EDAD EN UNA COHORTE DE NIÑOS SUECOS, LA MITAD DE LOS CUALES DESARROLLO CARIES, PARA ESTUDIAR EL DESARROLLO DE LA MICROBIOTA ASOCIADA A BUENA SALUD BUCODENTAL Y AQUELLA CARIOGENICA, MEDIANTE SECUENCIACION ILLUMINA DEL GEN RIBOSOMAL, ADEMAS DE MEDIR LA CARGA BACTERIANA Y FUNGICA A LO LARGO DEL TIEMPO Y LOS NIVELES DE INMUNOGLUBULINA A EN SALIVA. 2) CARACTERIZACION DE FUNCIONES BENEFICIOSAS POR PARTE DEL MICROBIOMA ORAL, EN CONCRETO EL EFECTO TAMPON SOBRE EL PH, Y LA REDUCCION DEL NITRATO. EL EFECTO TAMPONADOR DE LA PLACA DENTAL SE DEBE A RUTAS METABOLICAS COMO LA UREASA O LA ACTIVIDAD ARGINOLITICA, QUE DESEMBOCAN EN LA PRODUCCION DE AMONIO, EL CUAL NEUTRALIZA LOS ACIDOS CAUSANTES DE LA DESMINERALIZACION DEL ESMALTE. ADEMAS, EL PH NO SOLO AFECTA AL RIESGO DE CARIES DENTAL, SINO TAMBIEN A LA DIGESTION DE LOS CARBOHIDRATOS, DEBIDO A LA IMPORTANCIA DE LA PREDIGESTION QUE TIENE LUGAR EN LA BOCA, DONDE LA ALFA-AMILASA RESPONSABLE DE ESTE PROCESO TIENE UN PH OPTIMO DE 6,7 A 7. LA ACTIVIDAD NITRATO REDUCTASA DE LAS BACTERIAS ORALES ES TAMBIEN FUNDAMENTAL PARA LA PRODUCCION DE OXIDO NITRICO, EL CUAL TIENE UN CLARO EFECTO SISTEMICO SOBRE LA SALUD CARDIOVASCULAR, ENTRE OTROS IMPORTANTES EFECTOS FISIOLOGICOS; 3) CARACTERIZACION DE LA MICROBIOTA Y LAS RUTAS METABOLICAS ASOCIADAS A HALITOSIS, MEDIANTE SECUENCIACION MASIVA DEL METATRANSCRIPTOMA. ESTA ENFERMEDAD, QUE NO TIENE TODAVIA MEDIDAS TERAPEUTICAS EFECTIVAS, ESTA CAUSADA POR LA PRODUCCION DE COMPUESTOS VOLATILES POR PARTE DE BACTERIAS ORALES, Y LOS AGENTES ETIOLOGICOS DE LA MISMA NO ESTAN DILUCIDADOS, SI BIEN SE SABE QUE TIENE UN ORIGEN POLIMICROBIANO. MEDIANTE EL ESTUDIO BIOINFORMATICO DE LOS GENES EXPRESADOS EN CONDICIONES DE SALUD Y DE HALITOSIS, PODREMOS IDENTIFICAR LOS MICROORGANISMOS ACTIVOS Y LOS GENES EXPRESADOS RESPONSABLES DE LA ACTIVIDAD PATOGENICA._x000D_ ESTA INFORMACION SE UTILIZARA CON DOS FINES APLICADOS: _x000D_ 1) EL DESARROLLO DE KITS DE DIAGNOSTICO MOLECULAR QUE DETERMINEN LA CAPACIDAD TAMPON DE LA PLACA (ACTIVIDAD UREASA Y ARGININOLITICA) Y LA CAPACIDAD DE PRODUCCION DE NITRITO DEL MICROBIOMA ORAL (ACTIVIDAD NITRATO REDUCTORA); ESTOS TESTS SE UTILIZARAN PARA RELACIONAR LA CAPACIDAD TAMPON CON EL RIESGO DE CARIES Y CON PROBLEMAS DIGESTIVOS, ASI COMO PARA RELACIONAR LA CAPACIDAD DE PRODUCCION DE NITRITO CON LA PRESION SANGUINEA, QUE PODRIA EXPLICAR LA CORRELACION EPIDEMIOLOGICA ENTRE ENFERMEDADES ORALES Y RIESGO CARDIOVASCULAR. _x000D_ 2) LA SELECCION DE ESPECIES PROBIOTICAS, LAS CUALES SE AISLARAN DE INDIVIDUOS SIN LA PATOLOGIA CORRESPONDIENTE EN EL NICHO DONDE DEBEN EJERCER SU ACCION, Y QUE PODRAN SER UTILIZADOS COMO ESTRATEGIAS PREVENTIVAS FRENTE A LA CARIES, LA MALA ABSORCION DE CARBOHIDRATOS, LA HALITOSIS Y LAS ENFERMEDADES CARDIOVASCULARES. (Spanish)
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THE SET OF MICROORGANISMS THAT HABITUALLY INHABIT THE HUMAN BODY, KNOWN AS “MICROBIOMA”, PERFORMS A SERIES OF BENEFICIAL FUNCTIONS FOR OUR HEALTH, SOME OF WHICH ARE BEGINNING TO UNDERSTAND AND CHARACTERISE. IN THIS PROJECT, WE INTEND TO USE THE TECHNIQUES OF MASS SEQUENCING, METAGENOMICA AND MOLECULAR MICROBIOLOGY TO CHARACTERISE THE ORAL MICROBIOTA IN CONDITIONS OF HEALTH AND DISEASE, AND USE THIS INFORMATION FOR THE DEVELOPMENT OF DIAGNOSTIC AND PREVENTIVE STRATEGIES. THE PROJECT CONSISTS OF THREE MAIN WORK PACKAGES: 1) ORAL MICROBIOTA DEVELOPMENT FROM BIRTH TO 7 YEARS OF AGE IN CHILDREN WHO SUFFER AND DO NOT HAVE CAVITIES. SAMPLES AT 3, 6, 12, 24 AND 84 MONTHS OF AGE WILL BE USED IN A COHORT OF SWEDISH CHILDREN, HALF OF WHOM DEVELOP CARIES, TO STUDY THE DEVELOPMENT OF THE MICROBIOTA ASSOCIATED WITH GOOD ORAL HEALTH AND THAT CARIOGENICA, USING ILLUMINA SEQUENCING OF THE RIBOSOMAL GENE, IN ADDITION TO MEASURING BACTERIAL AND FUNGIC LOAD OVER TIME AND LEVELS OF IMMUNOGLUBULIN TO SALIVA. 2) CHARACTERISATION OF BENEFICIAL FUNCTIONS BY THE ORAL MICROBIOME, IN PARTICULAR THE TAMPON EFFECT ON PH, AND THE REDUCTION OF NITRATE. THE BUFFER EFFECT OF DENTAL PLAQUE IS DUE TO METABOLIC PATHWAYS SUCH AS UREASE OR ARGINOLITICA ACTIVITY, WHICH LEAD TO THE PRODUCTION OF AMMONIUM, WHICH NEUTRALISES THE ACIDS CAUSING THE DEMINERALISATION OF THE ENAMEL. IN ADDITION, PH NOT ONLY AFFECTS THE RISK OF DENTAL CARIES, BUT ALSO THE DIGESTION OF CARBOHYDRATES, DUE TO THE IMPORTANCE OF PREDIGESTION THAT TAKES PLACE IN THE MOUTH, WHERE THE ALPHA-AMYLASE RESPONSIBLE FOR THIS PROCESS HAS AN OPTIMAL PH OF 6.7 TO 7. THE NITRATE REDUCTASE ACTIVITY OF ORAL BACTERIA IS ALSO FUNDAMENTAL FOR THE PRODUCTION OF NITRIC OXIDE, WHICH HAS A CLEAR SYSTEMIC EFFECT ON CARDIOVASCULAR HEALTH, AMONG OTHER IMPORTANT PHYSIOLOGICAL EFFECTS; 3) CHARACTERISATION OF THE MICROBIOTA AND THE METABOLIC PATHWAYS ASSOCIATED WITH HALITOSIS, BY MASS SEQUENCING OF METATRANSCRIPTOMA. THIS DISEASE, WHICH DOES NOT YET HAVE EFFECTIVE THERAPEUTIC MEASURES, IS CAUSED BY THE PRODUCTION OF VOLATILE COMPOUNDS BY ORAL BACTERIA, AND THE ETHIOLOGIC AGENTS OF IT ARE NOT ELUCIDATED, ALTHOUGH IT IS KNOWN TO HAVE A POLYMICROBIAL ORIGIN. By means of the bio-informatics of GENES EXPRESED IN HEALTH AND HALITOSIS CONDITIONS, we may identify the active MICROORGANISMS AND EXPRESSED GENES RESPONSIBLE OF PATOGENICA ACTIVITY._x000D_ THIS INFORMATION WITH TWO FINES APPLICED: _x000D_ 1) THE DEVELOPMENT OF MOLECULAR DIAGNOSTIC KITS determining the TAMPON capacity of the platform (UREASA AND ARGININOLITIC ACTIVITY) and NITRIT PRODUCTION CAPACITY OF ORAL MICROBIOMA (REDUCTOR NITRATE ACTIVITY); THESE TESTS WERE USED TO RELATE TAMPON CAPACITY TO THE RISK OF CARIES AND DIGESTIVE PROBLEMS, AS WELL AS TO RELATE THE PRODUCTION CAPACITY OF NITRITE TO BLOOD PRESSURE, WHICH COULD EXPLAIN THE EPIDEMIOLOGICAL CORRELATION BETWEEN ORAL DISEASES AND CARDIOVASCULAR RISK. _x000D_ 2) SELECTION OF probiotic SPECIES, WHAT WHAT IS Isolated OF INDIVIDUUS WITHOUT THE PATOLOGY OF CORRESPONDIENT IN THE NICHOUSE WHERE WHERE YOUR ACCOUNT MUST BE YOUR ACCOUNT, AND YOU CAN BE USED AS PREVENTIVE STRATEGIES FROM THE CARIES, THE MALA ABSORCTION OF CARBOHYDRATS, HALITOSIS AND CARDIOVASCULAR DISEASES. (English)
12 October 2021
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Valencia
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Identifiers
BIO2015-68711-R
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