Q3135694 (Q3135694): Difference between revisions
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(Created claim: summary (P836): The application includes two equipment for the study of toxic microalgae at the cellular and molecular level, such as the analysis of images using confocal microscopy and the bioanalyser of nucleic acids with accessories for electrophoresis and flow cytometry. The applicant group has used a confocal microscope from the University of Vigo for the observation of dinoflagellates whose results have been used in the description of new toxic species....) |
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The application includes two equipment for the study of toxic microalgae at the cellular and molecular level, such as the analysis of images using confocal microscopy and the bioanalyser of nucleic acids with accessories for electrophoresis and flow cytometry. The applicant group has used a confocal microscope from the University of Vigo for the observation of dinoflagellates whose results have been used in the description of new toxic species. In recent years the cytogenetics line is being developed with the use of image cytometry and FISH techniques with epifluorescence microscopy that can be completed with the study of the chromosomes and their three-dimensional structure thanks to the high resolution confocal microscope. Conventional fluorescence microscopy resolution is limited by the principle of diffraction when light passes through optics. Only the fluorescence information contained in the focus plane can be obtained with a confocal microscope. Using this technique, in addition to an improvement in image quality, you can also do a more detailed analysis that allows a 3D reconstruction. The bioanalyser is an indispensable tool in today’s molecular biology laboratories. It can be used for electrophoretic separation (evaluate RNA and DNA quality), or for cell flow cytometry based on fluorescence. (English) | |||||||||||||||
| Property / summary: The application includes two equipment for the study of toxic microalgae at the cellular and molecular level, such as the analysis of images using confocal microscopy and the bioanalyser of nucleic acids with accessories for electrophoresis and flow cytometry. The applicant group has used a confocal microscope from the University of Vigo for the observation of dinoflagellates whose results have been used in the description of new toxic species. In recent years the cytogenetics line is being developed with the use of image cytometry and FISH techniques with epifluorescence microscopy that can be completed with the study of the chromosomes and their three-dimensional structure thanks to the high resolution confocal microscope. Conventional fluorescence microscopy resolution is limited by the principle of diffraction when light passes through optics. Only the fluorescence information contained in the focus plane can be obtained with a confocal microscope. Using this technique, in addition to an improvement in image quality, you can also do a more detailed analysis that allows a 3D reconstruction. The bioanalyser is an indispensable tool in today’s molecular biology laboratories. It can be used for electrophoretic separation (evaluate RNA and DNA quality), or for cell flow cytometry based on fluorescence. (English) / rank | |||||||||||||||
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| Property / summary: The application includes two equipment for the study of toxic microalgae at the cellular and molecular level, such as the analysis of images using confocal microscopy and the bioanalyser of nucleic acids with accessories for electrophoresis and flow cytometry. The applicant group has used a confocal microscope from the University of Vigo for the observation of dinoflagellates whose results have been used in the description of new toxic species. In recent years the cytogenetics line is being developed with the use of image cytometry and FISH techniques with epifluorescence microscopy that can be completed with the study of the chromosomes and their three-dimensional structure thanks to the high resolution confocal microscope. Conventional fluorescence microscopy resolution is limited by the principle of diffraction when light passes through optics. Only the fluorescence information contained in the focus plane can be obtained with a confocal microscope. Using this technique, in addition to an improvement in image quality, you can also do a more detailed analysis that allows a 3D reconstruction. The bioanalyser is an indispensable tool in today’s molecular biology laboratories. It can be used for electrophoretic separation (evaluate RNA and DNA quality), or for cell flow cytometry based on fluorescence. (English) / qualifier | |||||||||||||||
point in time: 12 October 2021
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Revision as of 13:11, 12 October 2021
Project Q3135694 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | No label defined |
Project Q3135694 in Spain |
Statements
2,883,696.8 Euro
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3,604,621.0 Euro
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80.0 percent
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1 January 2016
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30 June 2018
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INSTITUTO ESPAÑOL DE OCEANOGRAFIA. CENTRO OCEANOGRAFICO DE CADIZ
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42°14'15.58"N, 8°43'28.99"W
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36057
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La solicitud incluye dos equipos para el estudio de microalgas tóxicas a nivel celular y molecular, como son el análisis de imágenes mediante microscopía confocal y el bioanalizador de ácidos nucleicos con accesorios para electroforesis y citometría de flujo. El grupo solicitante ha utilizado un microscopio confocal de la Universidade de Vigo para la observación de dinoflagelados cuyos resultados han sido utilizados en la descripción de nuevas especies tóxicas. En los últimos años se está desarrollando la línea de citogenética con el uso de citometría de imagen y técnicas FISH con microscopía de epifluorescencia que podrá ser completada con el estudio de los cromosomas y su estructura tridimensional gracias al microscopio confocal de alta resolución. La resolución en microscopia de fluorescencia convencional esta limitada por el principio de difracción cuando la luz pasa a través de la óptica. Con un microscopio confocal se puede obtener sólo la información de fluorescencia que contiene el plano de foco. Mediante esta técnica, además de una mejora de la calidad de imagen, también se puede hacer un análisis más detallado que permite una reconstrucción en 3D. El bioanalizador es una herramienta indispensable en los laboratorios actuales de biología molecular. Puede emplearse para separación electroforética (evaluar calidad de ARN y ADN), o para citometría de flujo celular en base a fluorescencia. (Spanish)
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The application includes two equipment for the study of toxic microalgae at the cellular and molecular level, such as the analysis of images using confocal microscopy and the bioanalyser of nucleic acids with accessories for electrophoresis and flow cytometry. The applicant group has used a confocal microscope from the University of Vigo for the observation of dinoflagellates whose results have been used in the description of new toxic species. In recent years the cytogenetics line is being developed with the use of image cytometry and FISH techniques with epifluorescence microscopy that can be completed with the study of the chromosomes and their three-dimensional structure thanks to the high resolution confocal microscope. Conventional fluorescence microscopy resolution is limited by the principle of diffraction when light passes through optics. Only the fluorescence information contained in the focus plane can be obtained with a confocal microscope. Using this technique, in addition to an improvement in image quality, you can also do a more detailed analysis that allows a 3D reconstruction. The bioanalyser is an indispensable tool in today’s molecular biology laboratories. It can be used for electrophoretic separation (evaluate RNA and DNA quality), or for cell flow cytometry based on fluorescence. (English)
12 October 2021
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Vigo
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Identifiers
IEOC15-DE-3716
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