High performance and universal system for the digestion of DNA from biological samples (Q78508): Difference between revisions

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(‎Removed claim: summary (P836): The purpose of the project is to create and test (operational conditions) an innovative DNA digestion system from biological samples.A DNAza received in the course of the project will have features that allow it to be easily removed or inactivated after reaction and to be used in solutions with high concentrations of salt.Therefore, the project will respond to the market demand for DNAAs easily and efficiently in application.We will design and...)
Property / summary
The purpose of the project is to create and test (operational conditions) an innovative DNA digestion system from biological samples.A DNAza received in the course of the project will have features that allow it to be easily removed or inactivated after reaction and to be used in solutions with high concentrations of salt.Therefore, the project will respond to the market demand for DNAAs easily and efficiently in application.We will design and receive a fusion protein containing a modified hyperactivity sequence of human primates for the fusion of protein, which will allow protein to be assigned to the deposit after reaction.The original modifications of the sequence will result in efficient exports of the protein to a bacterial perplasma and its release into the breeding medium.The treatment of protein from a medium will enable simple and relatively cheap protein to be obtained.A key step in the research will be the optimisation of the express system in order to get the maximum yield of protein production.In the next steps we will work to improve the scale of the over-expression and purification to develop a method of obtaining a protein on a compound scale.The development will be carried out in order to test the nuclear activities in the targeted applications and to develop protocols for users.The final result of the project will be a complete protocol for obtaining on a scale production and use of the nuclease with a treatment system.Reference_reference_programme_aids:SA.41471 (2015/X) _public:Article 25 of Commission Regulation (EC) No 651/2014 of 17 June 2014 declaring certain categories of aid compatible with the internal market in the application of Article 107 and 108 of the Treaty (OJ(OJ LEU L 187/1, 26.06.2014). (English)
 
Property / summary: The purpose of the project is to create and test (operational conditions) an innovative DNA digestion system from biological samples.A DNAza received in the course of the project will have features that allow it to be easily removed or inactivated after reaction and to be used in solutions with high concentrations of salt.Therefore, the project will respond to the market demand for DNAAs easily and efficiently in application.We will design and receive a fusion protein containing a modified hyperactivity sequence of human primates for the fusion of protein, which will allow protein to be assigned to the deposit after reaction.The original modifications of the sequence will result in efficient exports of the protein to a bacterial perplasma and its release into the breeding medium.The treatment of protein from a medium will enable simple and relatively cheap protein to be obtained.A key step in the research will be the optimisation of the express system in order to get the maximum yield of protein production.In the next steps we will work to improve the scale of the over-expression and purification to develop a method of obtaining a protein on a compound scale.The development will be carried out in order to test the nuclear activities in the targeted applications and to develop protocols for users.The final result of the project will be a complete protocol for obtaining on a scale production and use of the nuclease with a treatment system.Reference_reference_programme_aids:SA.41471 (2015/X) _public:Article 25 of Commission Regulation (EC) No 651/2014 of 17 June 2014 declaring certain categories of aid compatible with the internal market in the application of Article 107 and 108 of the Treaty (OJ(OJ LEU L 187/1, 26.06.2014). (English) / rank
Normal rank
 

Revision as of 12:51, 14 October 2020

Project in Poland financed by DG Regio
Language Label Description Also known as
English
High performance and universal system for the digestion of DNA from biological samples
Project in Poland financed by DG Regio

    Statements

    country
    Poland
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    EU contribution
    1,492,169.25 zloty
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    358,120.62 Euro
    exchange rate to Euro
    0.24 Euro
    point in time
    13 January 2020
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    budget
    2,003,876.6 zloty
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    480,930.38 Euro
    exchange rate to Euro
    0.24 Euro
    point in time
    13 January 2020
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    co-financing rate
    74.46 percent
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    start time
    1 April 2017
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    end time
    31 July 2019
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    beneficiary name (string)
    INSTYTUT BIOINFOBANK SP. Z O.O.
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    beneficiary
    Q2510363
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    coordinate location

    52°8'45.2"N, 17°23'51.7"E
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    summary
    Projekt ma na celu stworzenie i przetestowanie (w warunkach operacyjnych) innowacyjnego systemu wytrawiania DNA z próbek biologicznych. DNaza otrzymana w toku realizacji projektu będzie posiadać właściwości pozwalające na jej łatwe usunięcie lub inaktywację po reakcjach oraz stosowanie w roztworach o wysokim stężeniu soli. Tym samym rezultat projektu będzie odpowiedzią na zapotrzebowanie rynku na DNazy łatwe i wydajne w zastosowaniu. Zaprojektujemy i otrzymamy białko fuzyjne zawierające zmodyfikowaną sekwencję hiperaktywnej ludzkiej DNazy I. Partner fuzyjny stanowiący tag, umożliwi wiązanie białka do złoża, co będzie wykorzystane do usuwania nukleazy po reakcjach. Oryginalne modyfikacje sekwencji spowodują wydajny eksport białka do bakteryjnej periplazmy i jego uwalnianie do medium hodowlanego. Oczyszczanie białka z medium umożliwi proste i stosunkowo tanie otrzymywanie białka. Kluczowym etapem badań będzie optymalizacja systemu ekspresyjnego w celu uzyskania maksymalnej wydajności produkcji białka. W kolejnych etapach pracować będziemy nad podniesieniem skali nadekspresji i oczyszczania tak, aby opracować metodę otrzymywania białka na skalę preparatywną. Prace rozwojowe będą prowadzone celem przetestowania nukleazy w docelowych zastosowaniach i opracowania protokołów dla użytkowników. Efektem końcowym projektu będzie kompletny protokół otrzymywania na skalę produkcyjną i stosowania nukleazy z systemem oczyszczania. Numer_referencyjny_programu_pomocowego: SA.41471(2015/X) Przeznaczenie_pomocy_publicznej: art. 25 rozporządzenia KE nr 651/2014 z dnia 17 czerwca 2014 r. uznające niektóre rodzaje pomocy za zgodne z rynkiem wewnętrznym w stosowaniu art. 107 i 108 Traktatu (Dz. Urz. UE L 187/1 z 26.06.2014). (Polish)
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    Identifiers

    Polish Kohesio ID
    POIR.01.01.01-00-0975/16
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