GENOMICS AND EVOLUTION OF HOST SPECIFICITY IN PSEUDOMONAS SAVASTANOI: THE RACE LEVEL (Q3208129): Difference between revisions
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(Created claim: summary (P836): BACTERIAL DISEASES OF PLANTS CAUSE SIGNIFICANT ECONOMIC LOSSES; THEIR CONTROL IS DIFFICULT, AND IS EXPECTED TO BE MORE DIFFICULT IN THE FUTURE DUE TO EU RESTRICTIONS ON THE AUTHORISATION OF ACTIVE SUBSTANCES AVAILABLE FOR INTEGRATED CONTROL. PSEUDOMONAS SAVASTANOI PV. SAVASTANOI (PSV) PRODUCES AIR TUMORS IN THE HOST, CAUSING ONE OF THE MOST IMPORTANT OLIVE DISEASES, ALTHOUGH IT IS ALSO PATHOGENOUS IN A LARGE NUMBER OF PLANT SPECIES. IN ADDITION...) |
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GENOMICS AND EVOLUTION OF HOST SPECIFICITY IN PSEUDOMONAS SAVASTANOI: THE RACE LEVEL | |||
Revision as of 01:26, 13 October 2021
Project Q3208129 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | GENOMICS AND EVOLUTION OF HOST SPECIFICITY IN PSEUDOMONAS SAVASTANOI: THE RACE LEVEL |
Project Q3208129 in Spain |
Statements
114,950.0 Euro
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229,900.0 Euro
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50.0 percent
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1 January 2015
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30 September 2018
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UNIVERSIDAD PUBLICA DE NAVARRA
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42°49'6.42"N, 1°38'39.34"W
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31201
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LAS ENFERMEDADES BACTERIANAS DE LAS PLANTAS CAUSAN IMPORTANTES PERDIDAS ECONOMICAS; SU CONTROL ES DIFICIL, Y SE ESPERA QUE LO SEA MAS EN EL FUTURO DEBIDO A LAS RESTRICCIONES DE LA UE PARA LA AUTORIZACION DE MATERIAS ACTIVAS DISPONIBLES PARA CONTROL INTEGRADO. PSEUDOMONAS SAVASTANOI PV. SAVASTANOI (PSV) PRODUCE TUMORES AEREOS EN EL HUESPED, PROVOCANDO UNA DE LAS ENFERMEDADES MAS IMPORTANTES DE OLIVO, AUNQUE TAMBIEN ES PATOGENO EN UN GRAN NUMERO DE ESPECIES DE PLANTAS. ADEMAS, PSV SE HA REVELADO RECIENTEMENTE COMO UN PATOGENO EMERGENTE EN LA ESPECIE ORNAMENTAL MANDEVILLA SANDERI (DIPLADENIA). P. SAVASTANOI PV. PHASEOLICOLA (PPH) ES UN PATOGENO DE IMPORTANCIA ECONOMICA EN JUDIA Y UNO DE LOS MODELOS DE INVESTIGACION MAS RELEVANTES EN FITOBACTERIOLOGIA. UNA DE LAS ESTRATEGIAS DE CONTROL DE PPH MAS DESEABLES ES EL USO DE VARIEDADES DE JUDIA RESISTENTES. LOS AISLADOS DE PPH SE CLASIFICAN EN 9 RAZAS EN FUNCION A SUS INTERACCIONES CON 8 VARIEDADES DIFERENTES DE JUDIA, QUE SE HAN EXPLICADO POR LA INTERACCION DE 5 PARES EN RELACION GEN A GEN, AUNQUE ES POSIBLE REALIZAR UNA MAYOR DIFERENCIACION EN HASTA 14 RAZAS. AMBOS PATOSISTEMAS OFRECEN UNA OPORTUNIDAD SIN PRECEDENTES PARA ESTUDIAR LAS BASES MOLECULARES DE LA ESPECIFICIDAD DE HUESPED. POR UN LADO, AUNQUE PPH ES UN PATOVAR GENETICAMENTE HOMOGENEO, LOS DIFERENTES AISLADOS MUESTRAN DIFERENCIAS EN SU VIRULENCIA, ASPECTO QUE REFLEJA LA ESPECIFICIDAD DE RAZA DE LOS MISMOS. DE OTRO LADO, LOS DIFERENTES PATOVARES DE P. SAVASTANOI INDUCEN SINTOMAS SIMILARES (TUMORES) TANTO EN HUESPEDES LEÑOSOS COMO HERBACEOS. LA DISPONIBILIDAD DE LOS GENOMAS CERRADOS DE LAS CEPAS MODELO PPH 1448A Y PSV NCPPB 3335, ASI COMO LAS HERRAMIENTAS GENETICAS Y FUNCIONALES DESARROLLADAS DURANTE LA EJECUCION DE NUESTROS PROYECTOS ANTERIORES, CONVIERTEN A ESTAS BACTERIAS EN MODELOS IDEALES PARA EL ANALISIS DE LA ESPECIFICIDAD DE HUESPED Y LA EVOLUCION DE LA VIRULENCIA. ASI, EL ANALISIS GENOMICO COMPARATIVO Y FUNCIONAL DE CEPAS BACTERIANAS FILOGENETICAMENTE RELACIONADAS FACILITARA LA IDENTIFICACION DE DETERMINANTES DE PATOGENICIDAD A NIVEL DE PATOVAR Y DE RAZA. LOS OBJETIVOS PRINCIPALES DE ESTE PROYECTO SON: 1) IDENTIFICAR Y CARACTERIZAR FUNCIONALMENTE DIFERENCIAS EN EL CONJUNTO DE GENES DE VIRULENCIA DE LOS PATOVARES DE PSV Y LAS RAZAS DE PPH RELACIONADAS CON LA ESPECIFICIDAD DE HUESPED; Y 2) APLICAR LA INFORMACION OBTENIDA MEDIANTE GENOMICA COMPARATIVA Y FUNCIONAL PARA EL DESARROLLO DE HERRAMIENTAS DE DIAGNOSTICO DE LAS RAZAS DE PPH Y DEL AISLADO EMERGENTE DE PSV EN MANDEVILLA. LOS OBJETIVOS ESPECIFICOS SON: 1) ASIGNAR UN PATOVAR A LAS CEPAS DE P. SAVASTANOI AISLADAS DE M. SANDERI. 2) SECUENCIAR Y ANOTAR LOS GENOMAS DE CEPAS REPRESENTATIVAS DE LOS PATOVARES Y LAS RAZAS DE P. SAVASTANOI. 3) REALIZAR UN ANALISIS GENOMICO COMPARATIVO Y UN ANALISIS FILOGENETICO DE LOS PATOVARES DE PSV Y DE LAS RAZAS DE PPH. 4) ESTUDIAR LA BIOLOGIA Y PAPEL EN LA ESPECIFICIDAD DE HUESPED DE LOS PLASMIDOS DE ESTAS CEPAS BACTERIANAS. 5) PROFUNDIZAR EN EL PAPEL EN LA ESPECIFICIDAD DE HUESPED DE LOS SISTEMAS DE SECRECION, DE LOS EFECTORES DEL SISTEMA DE SECRECION TIPO III Y, 6) DE OTROS FACTORES DE VIRULENCIA. 7) ESTUDIAR EL PAPEL DE LA EXPRESION GENICA EN LA ESPECIFICIDAD DE HUESPED. 8) DESARROLLAR HERRAMIENTAS DE DIAGNOSTICO ESPECIFICAS PARA LOS AISLADOS DE MANDEVILLA Y PARA LAS RAZAS DE PPH. (Spanish)
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BACTERIAL DISEASES OF PLANTS CAUSE SIGNIFICANT ECONOMIC LOSSES; THEIR CONTROL IS DIFFICULT, AND IS EXPECTED TO BE MORE DIFFICULT IN THE FUTURE DUE TO EU RESTRICTIONS ON THE AUTHORISATION OF ACTIVE SUBSTANCES AVAILABLE FOR INTEGRATED CONTROL. PSEUDOMONAS SAVASTANOI PV. SAVASTANOI (PSV) PRODUCES AIR TUMORS IN THE HOST, CAUSING ONE OF THE MOST IMPORTANT OLIVE DISEASES, ALTHOUGH IT IS ALSO PATHOGENOUS IN A LARGE NUMBER OF PLANT SPECIES. IN ADDITION, PSV HAS RECENTLY BEEN REVEALED AS AN EMERGING PATHOGEN IN THE ORNAMENTAL SPECIES MANDEVILLA SANDERI (DIPLADENIA). P. SAVASTANOI PV. PHASEOLICOLA (PPH) IS A PATHOGEN OF ECONOMIC IMPORTANCE IN JUDIA AND ONE OF THE MOST RELEVANT RESEARCH MODELS IN PHYTOBACTERIOLOGY. ONE OF THE MOST DESIRABLE PPH CONTROL STRATEGIES IS THE USE OF RESISTANT JEWISH VARIETIES. PPH ISOLATES ARE CLASSIFIED INTO 9 BREEDS ACCORDING TO THEIR INTERACTIONS WITH 8 DIFFERENT JEWISH VARIETIES, WHICH HAVE BEEN EXPLAINED BY THE INTERACTION OF 5 PAIRS IN GENE-TO-GEN RELATIONSHIP, ALTHOUGH IT IS POSSIBLE TO MAKE A GREATER DIFFERENTIATION IN UP TO 14 BREEDS. BOTH PATHOSYSTEMS OFFER AN UNPRECEDENTED OPPORTUNITY TO STUDY THE MOLECULAR BASES OF GUEST SPECIFICITY. ON THE ONE HAND, ALTHOUGH PPH IS A GENETICALLY HOMOGENOUS PATOVAR, THE DIFFERENT ISOLATES SHOW DIFFERENCES IN THEIR VIRULENCE, AN ASPECT THAT REFLECTS THEIR RACE SPECIFICITY. ON THE OTHER HAND, THE DIFFERENT PATOVARES OF P. SAVASTANOI INDUCE SIMILAR SYMPTOMS (TUMORS) IN BOTH WOODY AND HERBACEOUS HOSTS. THE AVAILABILITY OF THE CLOSED GENOMES OF THE PPH 1448A AND PSV NCPPB 3335 STRAINS, AS WELL AS THE GENETIC AND FUNCTIONAL TOOLS DEVELOPED DURING THE EXECUTION OF OUR PREVIOUS PROJECTS, MAKE THESE BACTERIA IDEAL MODELS FOR THE ANALYSIS OF GUEST SPECIFICITY AND THE EVOLUTION OF VIRULENCE. THUS, THE COMPARATIVE AND FUNCTIONAL GENOMIC ANALYSIS OF PHYLOGENETICALLY RELATED BACTERIAL STRAINS WILL FACILITATE THE IDENTIFICATION OF PATHOGENICITY DETERMINANTS AT THE PATOVAR AND BREED LEVEL. THE MAIN OBJECTIVES OF THIS PROJECT ARE: 1) FUNCTIONALLY IDENTIFY AND CHARACTERISE DIFFERENCES IN THE SET OF VIRULENCE GENES OF PSV PATHOVARS AND PPH BREEDS RELATED TO HOST SPECIFICITY; AND 2) APPLY THE INFORMATION OBTAINED BY COMPARATIVE AND FUNCTIONAL GENOMIC FOR THE DEVELOPMENT OF DIAGNOSTIC TOOLS FOR THE PPH BREEDS AND THE EMERGING ISOLATED PSV IN MANDEVILLA. THE SPECIFIC OBJECTIVES ARE: 1) ASSIGN A PATOVAR TO P. SAVASTANOI STRAINS ISOLATED FROM M. SANDERI. 2) SEQUENCE AND RECORD GENOMES OF REPRESENTATIVE STRAINS OF PATOVARS AND BREEDS OF P. SAVASTANOI. 3) PERFORM A COMPARATIVE GENOMIC ANALYSIS AND A PHYLOGENETIC ANALYSIS OF PSV PATHOVARS AND PPH BREEDS. 4) STUDY THE BIOLOGY AND ROLE IN THE SPECIFICITY OF HOST OF THE PLASMAIDS OF THESE BACTERIAL STRAINS. 5) TO DEEPEN THE ROLE IN THE GUEST SPECIFICITY OF THE SECRETION SYSTEMS, OF THE EFFECTORS OF THE TYPE III SECRETION SYSTEM AND, 6) OF OTHER VIRULENCE FACTORS. 7) TO STUDY THE ROLE OF GENIC EXPRESSION IN THE SPECIFICITY OF GUESTS. 8) DEVELOP SPECIFIC DIAGNOSTIC TOOLS FOR MANDEVILLA ISOLATES AND PPH BREEDS. (English)
13 October 2021
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Pamplona/Iruña
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Identifiers
AGL2014-53242-C2-2-R
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