HIGH-PERFORMANCE DEVICE DESIGN BASED ON LIGHT SHEET FLUORESCENCE MICROSOPIA FOR BIOMEDICAL APPLICATIONS (Q3208014): Difference between revisions
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(Created claim: summary (P836): HIGH PERFORMANCE ANALYSIS (HAR) IS A PHARMACO DISCOVERY PROCESS WIDELY USED IN THE PHARMACEUTICAL INDUSTRY. BY AUTOMATING THE PROCESSES IT IS POSSIBLE TO ANALYSE QUICKLY THE BIOLOGICAL OR BIOCHEMICAL ACTIVITY OF A LARGE NUMBER OF PHARMACOLOGICAL COMPOUNDS. HOWEVER, TODAY THERE IS A NEED TO IMPROVE THE MODELS AND TOOLS AVAILABLE TO PREDICT THE EFFICACY AND SAFETY OF NEW MEDICINES. In this connection, additions of the ANIMAL MODEL CLASSICS, new re...) |
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HIGH-PERFORMANCE DEVICE DESIGN BASED ON LIGHT SHEET FLUORESCENCE MICROSOPIA FOR BIOMEDICAL APPLICATIONS | |||
Revision as of 01:04, 13 October 2021
Project Q3208014 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | HIGH-PERFORMANCE DEVICE DESIGN BASED ON LIGHT SHEET FLUORESCENCE MICROSOPIA FOR BIOMEDICAL APPLICATIONS |
Project Q3208014 in Spain |
Statements
8,827,198.0 Euro
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17,654,396.0 Euro
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50.0 percent
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1 October 2015
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30 September 2018
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INSTITUTO DE CIENCIAS FOTONICAS
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41°17'9.96"N, 1°58'56.71"E
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08056
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EL ANALISIS DE ALTO RENDIMIENTO (AAR) ES UN PROCESO DE DESCUBRIMIENTO DE FARMACOS AMPLIAMENTE UTILIZADO EN LA INDUSTRIA FARMACEUTICA. MEDIANTE LA AUTOMATIZACION DE LOS PROCESOS SE CONSIGUE ANALIZAR RAPIDAMENTE LA ACTIVIDAD BIOLOGICA O BIOQUIMICA DE UN GRAN NUMERO DE COMPUESTOS FARMACOLOGICOS. SIN EMBARGO, HOY EN DIA EXISTE UNA NECESIDAD DE MEJORA DE LOS MODELOS Y LAS HERRAMIENTAS DISPONIBLES PARA PREDECIR LA EFICACIA Y SEGURIDAD DE NUEVOS MEDICAMENTOS. EN ESE CONTEXTO, ADEMAS DE LOS CLASICOS MODELOS ANIMALES, APARECEN NUEVAS HERRAMIENTAS DE INVESTIGACION COMO LOS MODELOS DE CULTIVO MULTICELULAR 3D, QUE IMITAN MODELOS DE TEJIDO EN CONDICIONES MAS FISIOLOGICAS._x000D_ SIN EMBARGO LA FALTA DE METODOS ANALITICOS DIFICULTA LA UTILIZACION GENERALIZADA DE ESTOS COMPLEJOS MODELOS. A FIN DE ALCANZAR EL PLENO SU POTENCIAL EN EL DESCUBRIMIENTO DE FARMACOS, EXISTE UNA NECESIDAD URGENTE DE DESARROLLO DE NUEVAS HERRAMIENTAS DE AAR DE ALTA RESOLUCION DURANTE LOS PROCESOS DE TRANSFECCION / TRANSDUCCION, LA DIFERENCIACION Y DE TINCION. ESTOS ANALISIS EXIGEN LA UTILIZACION MILES DE MUESTRAS BAJO DIFERENTES CONDICIONES, TIPOS DE PRUEBA Y CONTROLES. ES CRUCIAL LA AUTOMATIZACION DE ESTOS PROCESOS, LLEVANDOLOS A UNA ESCALA INDUSTRIAL, DE FORMA SENCILLA, MENOS COSTOSA Y MAS FIABLE. POR LO TANTO, EL DESARROLLO DE METODOLOGIAS INNOVADORAS DE ALTA RESOLUCION Y ALTA VELOCIDAD PERMITIRA LECTURAS MAS AMPLIAS, CONTRIBUYENDO A GENERAR MODELOS 3D BASADOS MAS PRECISOS Y FIABLES PARA PRUEBAS DE DETECCION DE DROGAS Y TOXICIDAD._x000D_ LAS TECNICAS BASADAS EN MICROSCOPIA CONFOCAL PARA APLICACIONES DE AAR SON INTRINSECAMENTE LENTAS. COMO ALTERNATIVA, SE HAN PROPUESTO LA MICROSCOPIA DE HOJA DE LUZ (LSFM EN INGLES) PARA SUPERAR ESTAS LIMITACIONES AL PROPORCIONAR VELOCIDADES DE ADQUISICION DE IMAGENES ULTRA-RAPIDAS. ADEMAS, MEDIANTE LA FUSION DE LAS VENTAJAS INHERENTES DE LSFM CON DISPOSITIVOS DE FLUJO, SERA POSIBLE REALIZAR ESTUDIOS MASIVOS DE CULTIVOS DE CELULAS VIVAS EN 3D. LO QUE SE PRETENDE CON ESTE PROYECTO ES EXPLORAR NUEVOS DISEÑOS Y PROTOTIPOS PARA LLEVAR ESTA TECNOLOGIA A SU MAXIMO POTENCIAL. CON ESTE ENFOQUE SEREMOS CAPACES DE HACER CON ALTA RESOLUCION, ALTA VELOCIDAD Y UN MINIMO DE DAÑO AAR CUANTITATIVOS DE LA ORGANIZACION ESPACIO-TEMPORAL DE LAS DIFERENTES LINEAS CELULARES EN ESFEROIDES Y OTROS MODELOS ANIMALES, COMO EL PEZ CEBRA O C. ELEGANS, ASI COMO SU RESPUESTA A DIFERENTES CONDICIONES AMBIENTALES. ESTO PERMITIRA SOFISTICADOS ENSAYOS PARA EL DIAGNOSTICO DE ENFERMEDADES, NUEVAS TERAPIAS, DETECCION DE DROGAS O DIFERENCIACION CELULAR, POR SOLO MENCIONAR ALGUNOS._x000D_ EL USO DE CULTIVOS CELULARES 3D Y LA AUTOMATIZACION COMPLETA DEL SISTEMA AAR PERMITIRA EVALUAR UN GRAN CONJUNTO DE PARAMETROS BIOLOGICOS CON RELEVANCIA ESTADISTICA. ALGUNOS DE LOS RETOS PARA LOS QUE ESTE TIPO DE EQUIPAMIENTO PUEDE CONTRIBUIR INCLUYEN LAS INVESTIGACIONES SOBRE LA RESPUESTA FARMACOLOGICA EN EL SISTEMA NERVIOSO CENTRAL, TERAPIAS CONTRA EL CANCER Y LA DIFERENCIACION CELULAR. NUESTRO OBJETIVO ULTIMO ES EL DESARROLLO DE EQUIPOS Y METODOS EN CAMPOS EN EXPANSION COMO EL DE CULTIVOS CELULARES 3D, LSFM Y LOS NUEVOS METODOS DE ANALISIS DE DROGAS QUE PERMITEN ASEGURAR LA COMPETITIVIDAD EN ESTAS AREAS, CON POSIBILIDADES DE EXPORTAR ESTA TECNOLOGIA A DIFERENTES LABORATORIOS DE INVESTIGACION Y LA INDUSTRIA FARMACEUTICA. LA PRESENTE PROPUESTA CONTRIBUIRA A MANTENER LA INVESTIGACION ESPAÑOLA EN LA VANGUARDIA DE LA CIENCIA BIOMEDICA, Y A SEGUIR APOYANDO A LA COMUNIDAD NACIONAL CON LOS MEJORES METODOS DISPONIBLES (Spanish)
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HIGH PERFORMANCE ANALYSIS (HAR) IS A PHARMACO DISCOVERY PROCESS WIDELY USED IN THE PHARMACEUTICAL INDUSTRY. BY AUTOMATING THE PROCESSES IT IS POSSIBLE TO ANALYSE QUICKLY THE BIOLOGICAL OR BIOCHEMICAL ACTIVITY OF A LARGE NUMBER OF PHARMACOLOGICAL COMPOUNDS. HOWEVER, TODAY THERE IS A NEED TO IMPROVE THE MODELS AND TOOLS AVAILABLE TO PREDICT THE EFFICACY AND SAFETY OF NEW MEDICINES. In this connection, additions of the ANIMAL MODEL CLASSICS, new research tools appear as well as multicellular 3D culture models, which mimic models of TEJID in more physical conditions._x000D_ WITHOUT THE FALTA OF Analytic Methods DIFFICULT THE GENERALised use of these modelling compounds. IN ORDER TO REACH ITS FULL POTENTIAL IN THE DISCOVERY OF PHARMACOS, THERE IS AN URGENT NEED FOR THE DEVELOPMENT OF NEW HIGH-RESOLUTION AAR TOOLS DURING THE TRANSFECCION/TRANSDUCCION PROCESSES, DIFFERENTIATION AND STAINING. THESE ANALYSES REQUIRE THE USE OF THOUSANDS OF SAMPLES UNDER DIFFERENT CONDITIONS, TEST TYPES AND CONTROLS. IT IS CRUCIAL TO AUTOMATE THESE PROCESSES, TAKING THEM ON AN INDUSTRIAL SCALE, IN A SIMPLE, LESS EXPENSIVE AND MORE RELIABLE WAY. Therefore, the development of innovative, high-resolution and high-velocity methods would allow for more extensive readings, leading to more accurate and reliable 3D models for drug and toxicity detection tests._x000D_ TECNICAL BASED IN MICROSCOPIA confocal FOR APPLICATIONS OF AAR ARE intrinsically slow. ALTERNATIVELY, LIGHT SHEET MICROSCOPY (LSFM) HAS BEEN PROPOSED TO OVERCOME THESE LIMITATIONS BY PROVIDING ULTRA-RAPED IMAGE ACQUISITION SPEEDS. MOREOVER, BY MERGING THE INHERENT ADVANTAGES OF LSFM WITH FLOW DEVICES, IT WILL BE POSSIBLE TO CARRY OUT MASSIVE STUDIES OF 3D LIVE CELL CULTURES. THE AIM OF THIS PROJECT IS TO EXPLORE NEW DESIGNS AND PROTOTYPES TO BRING THIS TECHNOLOGY TO ITS MAXIMUM POTENTIAL. WITH THIS APPROACH WE WILL BE ABLE TO DO WITH HIGH RESOLUTION, HIGH SPEED AND A MINIMUM OF QUANTITATIVE AAR DAMAGE OF THE SPATIAL-TEMPORAL ORGANISATION OF THE DIFFERENT CELL LINES IN SPHEROIDS AND OTHER ANIMAL MODELS, SUCH AS THE ZEBRAFISH OR C. ELEGANS, AS WELL AS ITS RESPONSE TO DIFFERENT ENVIRONMENTAL CONDITIONS. This will allow sophisticated DIAGNOSTIC DISEASE OF DISEASES, NEW TERAPIS, DOWNLOAD DETECTION OR CELL DIFFERENTATION, ONLY MENCING ALGUNOS._x000D_ The use of 3D CELULARY CULTIVES and the complete AAR system automation will allow to evaluate a great association of biologic parametres with statistic RELEVANCY. SOME OF THE CHALLENGES TO WHICH THIS TYPE OF EQUIPMENT CAN CONTRIBUTE INCLUDE RESEARCH ON PHARMACOLOGICAL RESPONSE IN THE CENTRAL NERVOUS SYSTEM, CANCER THERAPIES AND CELL DIFFERENTIATION. OUR LAST OBJECTIVE IS THE DEVELOPMENT OF EQUIPMENT AND METHODS IN FIELDS IN EXPANSION SUCH AS THE 3D CELL CULTURE, LSFM AND THE NEW METHODS OF DRUG ANALYSIS THAT ENSURE COMPETITIVENESS IN THESE AREAS, WITH POSSIBILITIES TO EXPORT THIS TECHNOLOGY TO DIFFERENT RESEARCH LABORATORIES AND THE PHARMACEUTICAL INDUSTRY. THIS PROPOSAL WILL CONTRIBUTE TO KEEPING SPANISH RESEARCH AT THE FOREFRONT OF BIOMEDICAL SCIENCE, AND TO CONTINUING TO SUPPORT THE NATIONAL COMMUNITY WITH THE BEST AVAILABLE METHODS. (English)
13 October 2021
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Castelldefels
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Identifiers
BIO2014-59614-JIN
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