Induction of regulatory T cells as an inflammatory control mechanism during selective intestinal decontamination as bacterial prophylaxis in cirrhosis (Q3180913): Difference between revisions
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(Created claim: summary (P836): Background: Norfloxacin modulates the inflammatory response in cirrhotic patients in selective intestinal decontamination through interleukin 10 (IL10). However, the mechanism by which norfloxacin is able to stimulate the production of this cytokine is unknown. Regulator T lymphocytes exert their modulating action by secreting IL10 and TGF-B and can be induced by dendritic cells activated through different receptors. Scenario: selective intestin...) |
(Changed label, description and/or aliases in en: translated_label) |
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Induction of regulatory T cells as an inflammatory control mechanism during selective intestinal decontamination as bacterial prophylaxis in cirrhosis | |||
Revision as of 19:29, 12 October 2021
Project Q3180913 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | Induction of regulatory T cells as an inflammatory control mechanism during selective intestinal decontamination as bacterial prophylaxis in cirrhosis |
Project Q3180913 in Spain |
Statements
42,750.0 Euro
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85,500.0 Euro
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50.0 percent
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1 January 2014
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31 March 2017
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FUNDACION PARA EL FOMENTO DE LA INV. SANITARIA Y BIOMEDICA DE LA COMUNIDAD VALENCIANA (FISABIO)
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38°20'37.10"N, 0°29'17.41"W
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03014
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Antecedentes: El norfloxacino modula la respuesta inflamatoria en pacientes cirróticos en descontaminación intestinal selectiva a través de la interleucina 10 (IL10). Sin embargo, se desconoce el mecanismo por el cual norfloxacino es capaz de estimular la producción de esta citocina. Los linfocitos T reguladores ejercen su acción moduladora secretando IL10 y TGF-B y pueden ser inducidos por células dendríticas activadas a través de distintos receptores. Hipótesis: la descontaminación intestinal selectiva con norfloxacino estimula la inducción de linfocitos T reguladores como mecanismo modulador de la respuesta inflamatoria en pacientes con cirrosis descompensada. Objetivo: Valorar la expresión y la función de linfocitos T reguladores como mecanismo de modulación inflamatoria durante la descontaminación intestinal selectiva con norfloxacino en la cirrosis descompensada. Diseño: Estudio observacional prospectivo sobre: a) pacientes con cirrosis y ascitis no neutrocítica con cultivo negativo y pacientes con cirrosis en descontaminación intestinal selectiva con norfloxacino como profilaxis secundaria de peritonitis bacteriana espontánea; b) ratones C57Bl/6J wild-type y ratones C57Bl/6J Rag1-/- con cirrosis inducida por CCl4 tratados con placebo, norfloxacino o trimetoprim-sulfametoxazol. Metodología: expresión y funcionalidad de células dendríticas y linfocitos T reguladores mediante cell sorting y citometría de flujo. Perfil de expresión génica de receptores Toll-like y NOD-like en células dendríticas y T reguladoras mediante PCR arrays. Pérdida/restauración de la modulación inflamatoria en ratones C57Bl/6J Rag1-/- con cirrosis mediante transfección con células T naive y controlado con células Treg de bazo de ratones C57Bl/6J wild-type. Cambios de composición en la microbiota intestinal asociados a norfloxacino mediante pirosecuenciación. (Spanish)
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Background: Norfloxacin modulates the inflammatory response in cirrhotic patients in selective intestinal decontamination through interleukin 10 (IL10). However, the mechanism by which norfloxacin is able to stimulate the production of this cytokine is unknown. Regulator T lymphocytes exert their modulating action by secreting IL10 and TGF-B and can be induced by dendritic cells activated through different receptors. Scenario: selective intestinal decontamination with norfloxacin stimulates the induction of regulatory T lymphocytes as a modulating mechanism of the inflammatory response in patients with decompensated cirrhosis. Objective: Assess the expression and function of regulatory T lymphocytes as a mechanism of inflammatory modulation during selective intestinal decontamination with norfloxacin in decompensated cirrhosis. Design: Prospective observational study on: patients with cirrhosis and non-neutralcytic ascites with negative culture and patients with cirrhosis in selective intestinal decontamination with norfloxacin as secondary prophylaxis of spontaneous bacterial peritonitis; C57Bl/6J wild-type mice and C57Bl/6J Rag1-/- mice with CCl4-induced cirrhosis treated with placebo, norfloxacin or trimethoprim-sulfamethoxazole. Methodology: expression and functionality of dendritic cells and T-cell regulators by cell sorting and flow cytometry. Gene expression profile of Toll-like and NOD-like receptors in dendritic and T-cell regulators using PCR arrays. Loss/restoration of inflammatory modulation in mice C57Bl/6J Rag1-/- with cirrhosis by transfection with T naive cells and controlled by C57Bl/6J wild-type mice spleen Treg cells. Changes in composition in the intestinal microbiota associated with norfloxacin by pyrossequencing. (English)
12 October 2021
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Alicante/Alacant
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Identifiers
PI13_01443
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