Effect of Holder pasteurisation on the content and speciation of trace elements in breast milk. Study of the bioavailability of dairy supplementation with Fe nanoparticles. (Q3153626): Difference between revisions
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(Created claim: summary (P836): Breast milk or, failing that, donated pasteurised human milk is the best option for feeding premature milk. Our main objective will be to evaluate the effect of Holder pasteurisation on the total content and speciation of the Faith, Se, Cu and Zn, as well as possible changes in protein profile in breast milk after pasteurisation. In other words, it is a question of scientifically investigating whether the necessities of trace elements in the neo...) |
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Effect of Holder pasteurisation on the content and speciation of trace elements in breast milk. Study of the bioavailability of dairy supplementation with Fe nanoparticles. | |||
Revision as of 15:22, 12 October 2021
Project Q3153626 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | Effect of Holder pasteurisation on the content and speciation of trace elements in breast milk. Study of the bioavailability of dairy supplementation with Fe nanoparticles. |
Project Q3153626 in Spain |
Statements
41,200.0 Euro
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51,500.0 Euro
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80.0 percent
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1 January 2017
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31 March 2020
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FUNDACION PARA LA INVESTIGACION E INNOVACION BIOSANITARIA EN EL PRINCIPADO DE ASTURIAS
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43°21'38.16"N, 5°50'41.64"W
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33044
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La leche materna o en su defecto la leche humana pasteurizada donada es la mejor opción para la alimentación del prematuro. Nuestro principal objetivo será evaluar el efecto de la pasteurización Holder en el contenido total y especiación del Fe, Se, Cu y Zn, así como posibles cambios originados en el perfil proteico en la leche materna tras la pasteurización. Es decir se trata de investigar científicamente si las necesidades de oligoelementos en el neonato, especialmente en el prematuro, están garantizadas tras el proceso de pasteurización. Otro objetivo, será evaluar la biodisponibilidad y el metabolismo del Fe administrado en forma de nanopartículas para la fortificación de la leche materna (y/o leche fórmula). Para ello se sintetizarán y caracterizarán nanopartículas (NPs) de oxo-hidróxido de Fe(III), utilizando isótopos estables (no radiactivos) de Fe y se administrarán a ratas lactantes. Finalmente, por técnicas de bioimagen se estudiará la distribución de Fe administrado como NPs en muestras de hígado de rata. La metodología para determinación total de Fe, Se, Cu y Zn, será la Espectrometría de Masas con Fuente de Plasma (ICP-MS). Los estudios de especiación se realizarán por “técnicas híbridas” basadas en el acoplamiento de la Cromatografía de Exclusión por Tamaños (SEC) al ICP-MS como detector. Para los estudios de biodisponibilidad y metabolismo se empleará un isótopo estable del Fe (57Fe) con el que se sintetizarán las NPs de oxo-hidróxido de 57Fe(III), que se administrarán a ratas lactantes como suplemento de la leche fórmula. Así mismo se analizará el contenido de Fe presente en diferentes fluidos y tejidos, y se cuantificará el Fe endógeno (natFe) y exógeno (57Fe) mediante metodología basada en deconvolución de perfiles isotópicos (IPD). Posteriormente, se identificarán los complejos proteína-Fe mayoritarios presentes en eritrocitos, hígado y suero por MALDI-TOF y ESI-TOF y finalmente se estudiará mediante técnica de bio-imagen (ablación laser ac (Spanish)
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Breast milk or, failing that, donated pasteurised human milk is the best option for feeding premature milk. Our main objective will be to evaluate the effect of Holder pasteurisation on the total content and speciation of the Faith, Se, Cu and Zn, as well as possible changes in protein profile in breast milk after pasteurisation. In other words, it is a question of scientifically investigating whether the necessities of trace elements in the neonate, especially in the premature, are guaranteed after the pasteurisation process. Another objective will be to evaluate the bioavailability and metabolism of Fe administered in the form of nanoparticles for the fortification of breast milk (or formula milk). To this end, Fe(III oxo-hydroxide nanoparticles (NPs) will be synthesised and characterised using stable (non-radioactive) Fe isotopes and administered to lactating rats. Finally, using bioimage techniques, the distribution of Fe administered as NPs in rat liver samples will be studied. The methodology for total determination of Fe, Se, Cu and Zn, will be the Plasma Source Mass Spectrometry (ICP-MS). Speciation studies shall be conducted by “hybrid techniques” based on the coupling of Size Exclusion Chromatography (SEC) to ICP-MS as a detector. For bioavailability and metabolism studies, a stable Fe isotope (57Fe) shall be used to synthesise 57Fe(III) oxohydroxide NPs to be administered to lactating rats as a formula supplement. Likewise, the content of Fe present in different fluids and tissues will be analysed, and endogenous faith (natFe) and exogenous (57Fe) will be quantified using a methodology based on the deconvolution of isotopic profiles (IPD). Subsequently, the majority protein-Fe complexes present in erythrocytes, liver and serum by MALDI-TOF and ESI-TOF will be identified and finally studied using bio-image technique (laser ac opening (English)
12 October 2021
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Oviedo
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Identifiers
PI16_02084
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