THE ACTIVITY OF THE ABCG2/BCRP MEMBRANE CONVEYOR IN RUMINANTS AS A POTENTIAL MARKER FOR MILK QUALITY AND SAFETY (Q3157082): Difference between revisions
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(Created claim: summary (P836): FOOD QUALITY AND SAFETY ARE PRIORITIES IN EUROPEAN SOCIETY AND ARE THEREFORE PART OF THE CHALLENGES PROPOSED BY THE EU UNDER THE HORIZON 2020 PROGRAMME. IN THIS PROJECT WE INTEND TO STUDY HOW THE ACTIVITY OF THE MEMBRANE CONVEYOR ABCG2/BCRP IN RUMINANTS CAN AFFECT THE QUALITY AND SAFETY OF A FUNDAMENTAL FOOD IN OUR DIET SUCH AS MILK. THE TRANSPORTER, INVOLVED IN THE TRANSFER TO MILK OF COMPOUNDS INCLUDING PHARMACOS AND NUTRIENTS, CAN BE MODULABL...) |
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THE ACTIVITY OF THE ABCG2/BCRP MEMBRANE CONVEYOR IN RUMINANTS AS A POTENTIAL MARKER FOR MILK QUALITY AND SAFETY | |||
Revision as of 15:14, 12 October 2021
Project Q3157082 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | THE ACTIVITY OF THE ABCG2/BCRP MEMBRANE CONVEYOR IN RUMINANTS AS A POTENTIAL MARKER FOR MILK QUALITY AND SAFETY |
Project Q3157082 in Spain |
Statements
51,425.0 Euro
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102,850.0 Euro
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50.0 percent
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1 January 2016
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31 December 2018
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UNIVERSIDAD DE LEON
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42°38'2.94"N, 5°58'17.11"W
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24089
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LA CALIDAD Y SEGURIDAD ALIMENTARIAS CONSTITUYEN PRIORIDADES EN LA SOCIEDAD EUROPEA Y POR TANTO FORMAN PARTE DE LOS RETOS PROPUESTOS POR LA UE DENTRO DEL PROGRAMA HORIZONTE 2020. EN EL PRESENTE PROYECTO PRETENDEMOS ESTUDIAR COMO LA ACTIVIDAD DEL TRANSPORTADOR DE MEMBRANA ABCG2/BCRP EN RUMIANTES PUEDE AFECTAR A LA CALIDAD Y SEGURIDAD DE UN ALIMENTO FUNDAMENTAL EN NUESTRA DIETA COMO ES LA LECHE. EL TRANSPORTADOR, IMPLICADO EN LA TRANSFERENCIA A LECHE DE COMPUESTOS ENTRE LOS QUE SE INCLUYEN FARMACOS Y NUTRIENTES, PUEDE SER MODULABLE Y, PRESENTA UN POLIMORFISMO EN GANADO VACUNO (Y581S) QUE DA LUGAR A UNA MAYOR SECRECION DE ANTIMICROBIANOS EN LOS ANIMALES PORTADORES. PARA LOGRAR NUESTRO OBJETIVO, REALIZAREMOS UN ANALISIS METABOLOMICO EXHAUSTIVO DE LA LECHE PROCEDENTE DE ANIMALES PORTADORES Y NO PORTADORES CON EL FIN DE ENCONTRAR NUEVOS COMPUESTOS, SUSTRATOS DEL TRANSPORTADOR. LA INTERACCION Y MODULACION DE ABCG2 SE ESTUDIARA IN VITRO CON CANDIDATOS "POTENCIALES" PRESENTES EN LA DIETA (ENTEROLIGNANOS Y SUS METABOLITOS, DISTINTOS TIPOS DE FITOESTROGENOS Y FLAVOLIGNANOS COMO LA SILIMARINA, Y LOS TANINOS) Y ENDOGENOS (TRIPTOFANO Y SUS METABOLITOS, Y DERIVADOS CONJUGADOS DE LA ESTRONA Y EL ESTRADIOL). TODOS ESTOS COMPUESTOS SON ESPECIALMENTE RELEVANTES POR EL VALOR AÑADIDO QUE APORTAN A LA LECHE DEBIDO A SU FUNCION ANTIOXIDANTE, HEPATOPROTECTORA Y PREVENTIVA EN EL CANCER COLORECTAL Y, FINALMENTE, LA PRESENCIA EN LA LECHE DE BOVINO DE ALGUNOS FARMACOS ANTINFLAMATORIOS NO ESTEROIDEOS (NSAIDS) REGULADA POR COMMISSION DECISION 2002/657/EC, (OFF J EUR COMMUNITIES L221: 8-36) JUSTIFICA LA IMPORTANCIA DE SU INCLUSION EN EL PRESENTE PROYECTO. SE UTILIZARAN CULTIVOS CELULARES, PREVIAMENTE GENERADOS EN PROYECTOS ANTERIORES, QUE SOBREEXPRESAN LA VARIANTE OVINA Y LAS DOS VARIANTES BOVINAS DEL TRANSPORTADOR, REALIZANDOSE EXPERIMENTOS DE TRANSPORTE TRANSEPITELIAL Y ENSAYOS DE ACUMULACION DE MITOXANTRONA. SERAN ESTUDIADAS, EN CADA CASO, LAS POSIBLES INHIBICIONES E INTERACCIONES FARMACOLOGICAS MEDIADAS POR EL TRANSPORTADOR. LA MAYOR O MENOR ACTIVIDAD DEL TRANSPORTADOR REGULA EL PASO DE COMPUESTOS A LECHE E INFLUYE SOBRE LA CALIDAD Y SEGURIDAD DE LA LECHE DE RUMIANTES; ESTA ACCION FINAL SE COMPROBARA EVALUANDO IN VIVO EL EFECTO DE LOS COMPUESTOS IDENTIFICADOS EN LOS ENSAYOS IN VITRO CON LOS CULTIVOS, Y UTILIZANDO EL MODELO DE RATONES KNOCKOUT EN EL TRANSPORTADOR. EN RUMIANTES, TANTO EN VACAS PORTADORAS Y581S COMO NO PORTADORAS, SE ANALIZARA LA CORRELACION LECHE/PLASMA DE LOS COMPUESTOS ENDOGENOS, DE LOS COMPUESTOS ADMINISTRADOS DIRECTAMENTE A LOS ANIMALES O FORMANDO PARTE DE SU DIETA. SE INCLUIRAN ENSAYOS DE INHIBICION DE SECRECION EN LECHE EN OVINO Y BOVINO, CUANDO SE IDENTIFIQUEN INHIBIDORES DEL TRANSPORTADOR. EL ESFUERZO PARA PONER LOS MEDIOS NECESARIOS EN EL ESTUDIO DE LA CALIDAD Y SEGURIDAD DE LA LECHE ESTA JUSTIFICADO AL SER UN ALIMENTO PRIMORDIAL DE ELEVADO CONSUMO, PERO ESTOS ESTUDIOS TAMBIEN BENEFICIAN, Y DE MANERA IMPORTANTE, A LA INDUSTRIA LACTEA Y A LOS GANADEROS, VINCULANDO LAS MEJORAS EN LA PRODUCCION GANADERA A LOS RETOS DE LA SOCIEDAD. (Spanish)
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FOOD QUALITY AND SAFETY ARE PRIORITIES IN EUROPEAN SOCIETY AND ARE THEREFORE PART OF THE CHALLENGES PROPOSED BY THE EU UNDER THE HORIZON 2020 PROGRAMME. IN THIS PROJECT WE INTEND TO STUDY HOW THE ACTIVITY OF THE MEMBRANE CONVEYOR ABCG2/BCRP IN RUMINANTS CAN AFFECT THE QUALITY AND SAFETY OF A FUNDAMENTAL FOOD IN OUR DIET SUCH AS MILK. THE TRANSPORTER, INVOLVED IN THE TRANSFER TO MILK OF COMPOUNDS INCLUDING PHARMACOS AND NUTRIENTS, CAN BE MODULABLE AND HAS A POLYMORPHISM IN CATTLE (Y581S) WHICH RESULTS IN GREATER SECRETION OF ANTIMICROBIALS IN CARRIER ANIMALS. TO ACHIEVE OUR GOAL, WE WILL CARRY OUT A THOROUGH METABOLOMIC ANALYSIS OF MILK FROM CARRIER AND NON-CARRYING ANIMALS IN ORDER TO FIND NEW COMPOUNDS, SUBSTRATES OF THE TRANSPORTER. THE INTERACTION AND MODULATION OF ABCG2 WILL BE STUDIED IN VITRO WITH “POTENTIAL” CANDIDATES PRESENT IN THE DIET (ENTEROLIGNANS AND THEIR METABOLITES, DIFFERENT TYPES OF PHYTOESTROGENS AND FLAVOLIGNANS SUCH AS SILYMARIN, TANNINS) AND ENDOGENES (TRYPTOPHAN AND ITS METABOLITES, AND CONJUGATED DERIVATIVES OF ESTRONE AND ESTRADIOL). ALL THESE COMPOUNDS ARE PARTICULARLY RELEVANT FOR THE ADDED VALUE THEY BRING TO MILK DUE TO THEIR ANTIOXIDANT, HEPATOPROTECTIVE AND PREVENTIVE FUNCTION IN COLORECTAL CANCER AND, FINALLY, THE PRESENCE IN BOVINE MILK OF SOME NONSTEROIDAL ANTI-INFLAMMATORY DRUGS (NSAIDS) REGULATED BY COMMISSION DECISION 2002/657/EC (OFF J EUR COMMUNITIES L221: 8-36) JUSTIFIES THE IMPORTANCE OF ITS INCLUSION IN THIS PROJECT. CELL CULTURES, PREVIOUSLY GENERATED IN PREVIOUS PROJECTS, WILL BE USED THAT OVEREXPRESS THE OVINE VARIANT AND THE TWO BOVINE VARIANTS OF THE TRANSPORTER, CARRYING OUT TRANSEPITHELIAL TRANSPORT EXPERIMENTS AND MITOXANTRONE ACCUMULATION TESTS. POSSIBLE INHIBITIONS AND PHARMACOLOGICAL INTERACTIONS MEDIATED BY THE TRANSPORTER SHALL BE STUDIED IN EACH CASE. THE INCREASED OR LESSER ACTIVITY OF THE CONVEYOR REGULATES THE PASSAGE FROM COMPOUNDS TO MILK AND INFLUENCES THE QUALITY AND SAFETY OF RUMINANT MILK; THIS FINAL ACTION WILL BE CHECKED BY ASSESSING IN VIVO THE EFFECT OF THE COMPOUNDS IDENTIFIED IN THE IN VITRO ASSAYS WITH THE CULTURES, AND USING THE KNOCKOUT MICE MODEL ON THE TRANSPORTER. IN RUMINANTS, BOTH IN Y581S AND NON-CARRYING COWS, THE MILK/PLASM CORRELATION OF THE ENDOGENOUS COMPOUNDS, OF THE COMPOUNDS ADMINISTERED DIRECTLY TO THE ANIMALS OR AS PART OF THEIR DIET, WILL BE ANALYSED. TESTS OF INHIBITION OF SECRETION IN MILK IN SHEEP AND BOVINE ANIMALS SHALL BE INCLUDED WHEN INHIBITORS OF THE TRANSPORTER ARE IDENTIFIED. THE EFFORT TO PUT THE NECESSARY RESOURCES INTO THE STUDY OF THE QUALITY AND SAFETY OF MILK IS JUSTIFIED AS IT IS A PRIMARY FOOD OF HIGH CONSUMPTION, BUT THESE STUDIES ALSO BENEFIT, AND IMPORTANTLY, THE LACTEA INDUSTRY AND FARMERS, LINKING IMPROVEMENTS IN LIVESTOCK PRODUCTION TO THE CHALLENGES OF SOCIETY. (English)
12 October 2021
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León
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Identifiers
AGL2015-65626-R
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