MECHANISMS FOR REGULATING LIPIDIC METABOLISM AND ITS RELATION TO STRESS RESPONSE IN SACCHAROMYCES CEREVISIAE (Q3144507): Difference between revisions
Jump to navigation
Jump to search
(Created claim: summary (P836): THE LIPIDIC COMPOSITION OF THE PLASMATIC MEMBRANE IS KEY TO NUMEROUS CELLULAR PROCESSES THAT DEPEND ON ITS FUNCTIONALITY, IN THE RECEPTION AND TRANSMISSION OF SIGNALS AND IN THE RESPONSE AND ADAPTATION TO ENVIRONMENTAL DISTURBANCES. KNOWLEDGE OF ITS REGULATORY MECHANISMS IS THEREFORE FUNDAMENTAL. THE POSSIBILITY OF REGULATING THE LIPID CONTENT OF S. CEREVISIAE ALSO OPENS UP THE POSSIBILITY OF OBTAINING YEASTS WITH GREATER TOLERANCE TO STRESS AND...) |
(Changed label, description and/or aliases in en: translated_label) |
||
| label / en | label / en | ||
MECHANISMS FOR REGULATING LIPIDIC METABOLISM AND ITS RELATION TO STRESS RESPONSE IN SACCHAROMYCES CEREVISIAE | |||
Revision as of 14:07, 12 October 2021
Project Q3144507 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | MECHANISMS FOR REGULATING LIPIDIC METABOLISM AND ITS RELATION TO STRESS RESPONSE IN SACCHAROMYCES CEREVISIAE |
Project Q3144507 in Spain |
Statements
66,550.0 Euro
0 references
133,100.0 Euro
0 references
50.0 percent
0 references
1 January 2016
0 references
31 December 2019
0 references
AGENCIA CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
0 references
39°30'14.11"N, 0°26'31.56"W
0 references
46190
0 references
LA COMPOSICION LIPIDICA DE LA MEMBRANA PLASMATICA ES CLAVE PARA NUMEROSOS PROCESOS CELULARES QUE DEPENDEN DE SU FUNCIONALIDAD, EN LA RECEPCION Y TRANSMISION DE SEÑALES Y EN LA RESPUESTA Y ADAPTACION A PERTURBACIONES AMBIENTALES. EL CONOCIMIENTO DE SUS MECANISMOS DE REGULACION ES PUES FUNDAMENTAL. LA POSIBILIDAD DE REGULAR EL CONTENIDO EN LIPIDOS DE S. CEREVISIAE ABRE TAMBIEN LA POSIBILIDAD DE OBTENER LEVADURAS CON MAYOR TOLERANCIA A ESTRES Y NUEVOS BIOPRODUCTOS E INGREDIENTES BASADOS EN BIOMASA DE LEVADURA O DE SUS FRACCIONES, DE ACUERDO A LOS RETOS DEL PROGRAMA HORIZONTE 2020. EN ESTE ESCENARIO, NUESTRA PROPUESTA GIRA EN TORNO AL PAPEL DE LA RUTA PKC Y DE ALGUNOS LIPIDOS EN SEÑALIZACION Y HOMEOSTASIS LIPIDICA. RESULTADOS PREVIOS DE NUESTRO GRUPO HAN REVELADO COMO VARIACIONES EN EL CONTENIDO DE PI(4,5)P2 MEDIANTE MUTACION DEL GEN INP51 CAUSAN UNA REDUCCION DE LA ACTIVIDAD DEL COMPLEJO QUINASA PHO80-PHO85 Y EN LA ABUNDANCIA DE UNO DE SUS SUBSTRATOS, LA FOSFATIDATO FOSFATASA DE LEVADURA PAH1, UNA ENZIMA CLAVE EN LA BIOSINTESIS DE TRIGLICERIDOS Y FOSFOLIPIDOS. VARIACIONES EN EL NIVEL DE PI(4,5)P2 REGULAN EL CONTENIDO DE 1-IP7, UNA MOLECULA CONOCIDA POR SU ACTIVIDAD INHIBIDORA DE PHO85, Y ESTIMULAN LA ACTIVIDAD DE LA MAP QUINASA DE LA RUTA PKC, SLT2. NUESTRA HIPOTESIS ES QUE LOS NIVELES DE PI(4,5)P2 SON TAMBIEN SENSIBLES A PERTURBACIONES AMBIENTALES Y QUE EN RESPUESTA A ESTOS CAMBIOS, 1-IP7 Y SLT2 ORQUESTAN UNA REGULACION COORDINADA DE LA BIOSINTESIS DE GLICEROLIPIDOS, VIA PHO85-PAH1, QUE INCLUYE TAMBIEN EL METABOLISMO DE ESFINGOLIPIDOS. CREEMOS QUE LA ACTIVACION DE SLT2 PARTICIPA TAMBIEN EN EL CONTROL DE LA RUTA DE HEXOSAMINAS, VIA GFA1, Y PROBABLEMENTE DE OTROS EFECTORES. ESTA REGULACION ES FUNDAMENTAL EN EL APORTE DE N-ACETILGLUCOSAMINA, EL PRECURSOR, ENTRE OTROS, DE QUITINA, ANCLAS DE GPI Y DE GLICOPROTEINAS, Y EN LA ACTIVACION DE MECANISMOS DE RESPUESTA A ESTRES. DE ACUERDO A TODO ESTO, LA PROPUESTA PLANTEA EXPERIMENTOS PARA VALIDAR ESTA HIPOTESIS Y APROXIMACIONES BIOTECNOLOGICAS PARA OBTENER NUEVAS CEPAS DE LEVADURA, PRODUCTOS Y MOLECULAS DE INTERES INDUSTRIAL. (Spanish)
0 references
THE LIPIDIC COMPOSITION OF THE PLASMATIC MEMBRANE IS KEY TO NUMEROUS CELLULAR PROCESSES THAT DEPEND ON ITS FUNCTIONALITY, IN THE RECEPTION AND TRANSMISSION OF SIGNALS AND IN THE RESPONSE AND ADAPTATION TO ENVIRONMENTAL DISTURBANCES. KNOWLEDGE OF ITS REGULATORY MECHANISMS IS THEREFORE FUNDAMENTAL. THE POSSIBILITY OF REGULATING THE LIPID CONTENT OF S. CEREVISIAE ALSO OPENS UP THE POSSIBILITY OF OBTAINING YEASTS WITH GREATER TOLERANCE TO STRESS AND NEW BIOPRODUCTS AND INGREDIENTS BASED ON YEAST BIOMASS OR THEIR FRACTIONS, ACCORDING TO THE CHALLENGES OF THE HORIZON 2020 PROGRAMME. IN THIS SCENARIO, OUR PROPOSAL REVOLVES AROUND THE ROLE OF THE PKC ROUTE AND SOME LIPIDS IN SIGNAGE AND HOMEOSTASIS LIPIDICA. PREVIOUS RESULTS OF OUR GROUP HAVE REVEALED VARIATIONS IN THE CONTENT OF PI(4,5)P2 BY MUTATION OF THE INP51 GENE CAUSE A REDUCTION IN THE ACTIVITY OF THE PHO80-PHO85 KINASE COMPLEX AND IN THE ABUNDANCE OF ONE OF ITS SUBSTRATES, PAH1 YEAST PHOSPHATASE PHOATIDATE, A KEY ENZYME IN THE BIOSYNTHESIS OF TRIGLYCERIDES AND PHOSPHOLIPIDS. VARIATIONS IN THE LEVEL OF PI(4,5)P2 REGULATE THE CONTENT OF 1-IP7, A MOLECULATE KNOWN FOR ITS PHO85 INHIBITORY ACTIVITY, AND STIMULATE THE ACTIVITY OF THE MAP KINASE OF THE PKC ROUTE, SLT2. OUR HYPOTHESIS IS THAT PI(4,5)P2 LEVELS ARE ALSO SENSITIVE TO ENVIRONMENTAL DISTURBANCES AND THAT IN RESPONSE TO THESE CHANGES, 1-IP7 AND SLT2 ORCHESTRATE A COORDINATED REGULATION OF THE BIOSYNTHESIS OF GLICEROLIPIDS, VIA PHO85-PAH1, WHICH ALSO INCLUDES THE METABOLISM OF ESFINGOLIPIDS. WE BELIEVE THAT THE ACTIVATION OF SLT2 IS ALSO INVOLVED IN CONTROLLING THE ROUTE OF HEXOSAMINES, VIA GFA1, AND PROBABLY OTHER EFFECTORS. THIS REGULATION IS FUNDAMENTAL IN THE CONTRIBUTION OF N-ACETYLGLUCOSAMINE, THE PRECURSOR, AMONG OTHERS, OF CHITIN, ANCHORS OF GPI AND GLYCOPROTEINS, AND IN THE ACTIVATION OF MECHANISMS OF RESPONSE TO STRESS. ACCORDING TO ALL THIS, THE PROPOSAL PROPOSES EXPERIMENTS TO VALIDATE THESE BIOTECHNOLOGICAL HYPOTHESES AND APPROACHES TO OBTAIN NEW STRAINS OF YEAST, PRODUCTS AND MOLECULES OF INDUSTRIAL INTEREST. (English)
12 October 2021
0 references
Paterna
0 references
Identifiers
BIO2015-71059-R
0 references