PATHOGENICITY OF BETANODAVIRUS IN CULTURED SOLE (SOLEA SENEGALENSIS) AND ITS RELATION TO HOST IMMUNE RESPONSE (Q3134591): Difference between revisions
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(Created claim: summary (P836): VIRAL NERVE NECROSIS (NNV) IS A DISEASE THAT AFFECTS A VARIETY OF FISH WORLDWIDE, CAUSING HIGH MORTALITY RATES, ESPECIALLY IN LARVAE AND JUVENILES. THE ETHIOLOGIC AGENT OF THE DISEASE IS A BETANODAVIRUS, WHICH HAS A BISEGMENTED MONOCATENARY RNA GENOME COMPOSED OF RNA1, WHICH ENCODES VIRAL POLYMERASE, AND RNA2, WHICH ENCODES THE CAPSIDE PROTEIN. IN ADDITION, THEY SYNTHESISE A SUBGENOMIC RNA, RNA3, WHICH ENCODES PROTEINS B1 (ANTI-NECROTIC FACTOR)...) |
(Changed label, description and/or aliases in en: translated_label) |
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PATHOGENICITY OF BETANODAVIRUS IN CULTURED SOLE (SOLEA SENEGALENSIS) AND ITS RELATION TO HOST IMMUNE RESPONSE | |||
Revision as of 12:23, 12 October 2021
Project Q3134591 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | PATHOGENICITY OF BETANODAVIRUS IN CULTURED SOLE (SOLEA SENEGALENSIS) AND ITS RELATION TO HOST IMMUNE RESPONSE |
Project Q3134591 in Spain |
Statements
135,520.0 Euro
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169,400.0 Euro
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80.0 percent
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1 January 2015
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31 December 2018
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UNIVERSIDAD DE MALAGA
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36°43'16.68"N, 4°25'17.90"W
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29067
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LA NECROSIS NERVIOSA VIRAL (NNV) ES UNA ENFERMEDAD QUE AFECTA A GRAN VARIEDAD DE PECES EN TODO EL MUNDO, CAUSANDO ELEVADAS TASAS DE MORTALIDAD, SOBRE TODO EN LARVAS Y JUVENILES. EL AGENTE ETIOLOLOGICO DE LA ENFERMEDAD ES UN BETANODAVIRUS, QUE POSEE UN GENOMA RNA MONOCATENARIO BISEGMENTADO COMPUESTO POR EL RNA1, QUE CODIFICA LA POLIMERASA VIRAL, Y EL RNA2, QUE CODIFICA LA PROTEINA DE LA CAPSIDE. ADEMAS, SINTETIZAN UN RNA SUBGENOMICO, RNA3, QUE CODIFICA LAS PROTEINAS B1 (FACTOR ANTI-NECROTICO) Y B2 (PROTECCION DE LA REPLICACION VIRAL FRENTE A RNA INTERFERENTES CELULARES). LOS BETANODAVIRUS SE CLASIFICAN EN 4 GENOTIPOS SEGUN LA SECUENCIA T4 DEL RNA2: SJNNV, TPNNV, BFNNV Y RGNNV. SIN EMBARGO EN LOS ULTIMOS AÑOS SE HA DOCUMENTADO EL AISLAMIENTO DE RECOMBINANTES NATURALES RG/SJ (RNA1 TIPO RGNNV Y RNA2 SJNNV) EN EL SUR DE EUROPA. TODOS LOS RECOMBINATES AISLADOS DE LENGUADO Y DORADA CULTIVADOS EN LA PENINSULA IBERICA TIENEN UNA PROTEINA DE LA CAPSIDE CON 3 POSICIONES AMINOACIDICAS DIFERENTES RESPECTO A LA DE SJNNV, 2 DE ELLAS EN EL EXTREMO C-TERMINAL, REGION DETERMINANTE DE ESPECIFICIDAD DE HOSPEDADOR, QUE SE HA DEMOSTRADO JUEGAN UN PAPEL IMPORTANTE EN LA VIRULENCIA, Y UNA EN LA REGION N-TERMINAL. ASIMISMO, ESTOS RECOMBINANTES PRESENTAN EN LA POLIMERASA 19 RESIDUOS AMIOACIDICOS DIFERENTES CON RESPECTO A LA POLIMERASA DE LA CEPA TIPO RG, Y EN LAS REGIONES NO CODIFICANTES DE AMBOS SEGMENTOS GENOMICOS UN TOTAL DE 4 NUCLEOTIDOS QUE NO COINCIDEN CON LAS CEPAS TIPO RG (RNA1) Y SJ (RNA2). TODOS ESTOS CAMBIOS PUEDEN DESEMPEÑAR UN PAPEL IMPORTANTE EN LA VIRULENCIA Y EN LA CAPACIDAD REPLICATIVA. EL ESTUDIO DE LOS DETERMINANTES DE VIRULENCIA (RELACIONADA ADEMAS, CON LA POLIMERASA Y CON LOS ORF B1 Y B2) ES FUNDAMENTAL PARA ENTENDER Y CONTROLAR LA ENFERMEDAD, ASPECTOS QUE SERAN ABORDADOS POR EL EQUIPO DE INVESTIGACION DE LA USC. ES BIEN SABIDO QUE EL DESARROLLO DE LA ENFERMEDAD NO DEPENDE SOLO DEL PATOGENO, ESTANDO DETERMINADO POR LA INTERACCION VIRUS-HOSPEDADOR. LA IMPLICACION DEL HOSPEDADOR ESTA BASADA EN LA ESTIMULACION DEL SISTEMA INMUNE, QUE SERA CARACTERIZADO POR EL EQUIPO DE INVESTIGACION DE LA UMA. LA INFECCION VIRICA INDUCE LA ACTIVACION TANTO DEL SISTEMA INMUNE INNATO COMO DEL ADAPTATIVO. LOS ESTUDIOS SOBRE LA ACTIVACION DEL SISTEMA INMUNE QUE SE LLEVARAN A CABO INCLUYEN LA REALIZACION DE UNA SECUENCIACION MASIVA TRANSCRIPTOMICA (RNA-SEQ) EN RESPUESTA A INFECCIONES POR VIRUS DE DISTINTA VIRULENCIA PARA LENGUADO. ESTE ESTUDIO GENERARA UNA INFORMACION VALIOSISIMA SOBRE LOS INMUNOGENES IMPLICADOS EN RUTAS DE DEFENSA ANTIVIRICAS, AL TIEMPO QUE PERMITIRA EL DISEÑO DE OPENARRAYS BASADOS EN PCR A TIEMPO REAL CON SONDAS TAQMAN QUE SE UTILIZARAN PARA ESTUDIAR, A NIVEL DE EXPESION GENICA, LA RESPUESTA DEL SISTEMA INMUNE DE LENGUADO FRENTE A VIRUS RECOMBINANTES, OBTENIDOS POR GENETICA INVERSA, CON DISTINTO NIVEL DE VIRULENCIA._x000D_ ATENDIENDO A ESTA HIPOTESIS, EL OBJETIVO PRINCIPAL DEL PRESENTE PROYECTO ES ANALIZAR LOS FACTORES IMPLICADOS EN LA VIRULENCIA DE BETANODAVIRUS EN LENGUADO Y SU RELACION CON LA RESPUESTA INMUNE DEL HOSPEDADOR. (Spanish)
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VIRAL NERVE NECROSIS (NNV) IS A DISEASE THAT AFFECTS A VARIETY OF FISH WORLDWIDE, CAUSING HIGH MORTALITY RATES, ESPECIALLY IN LARVAE AND JUVENILES. THE ETHIOLOGIC AGENT OF THE DISEASE IS A BETANODAVIRUS, WHICH HAS A BISEGMENTED MONOCATENARY RNA GENOME COMPOSED OF RNA1, WHICH ENCODES VIRAL POLYMERASE, AND RNA2, WHICH ENCODES THE CAPSIDE PROTEIN. IN ADDITION, THEY SYNTHESISE A SUBGENOMIC RNA, RNA3, WHICH ENCODES PROTEINS B1 (ANTI-NECROTIC FACTOR) AND B2 (PROTECTION OF VIRAL REPLICATION AGAINST CELLULAR INTERFERING RNA). BETANODAVIRUSES ARE CLASSIFIED INTO 4 GENOTYPES ACCORDING TO THE T4 SEQUENCE OF RNA2: SJNNV, TPNNV, BFNNV AND RGNNV. HOWEVER, IN RECENT YEARS THE ISOLATION OF NATURAL RECOMBINANTS RG/SJ (RNA1 TYPE RGNNV AND RNA2 SJNNV) HAS BEEN DOCUMENTED IN SOUTHERN EUROPE. ALL ISOLATED RECOMBINANTS OF SOLE AND GOLD GROWN IN THE IBERIAN PENINSULA HAVE A CAPSIDE PROTEIN WITH 3 DIFFERENT AMINOACIDIC POSITIONS COMPARED TO THAT OF SJNNV, 2 OF THEM AT THE END C-TERMINAL, REGION DETERMINING HOST SPECIFICITY, WHICH HAS BEEN SHOWN TO PLAY AN IMPORTANT ROLE IN VIRULENCE, AND ONE IN THE N-TERMINAL REGION. IN ADDITION, THESE RECOMBINANTS PRESENT IN POLYMERASE 19 DIFFERENT AMIOACIDIC RESIDUES WITH RESPECT TO POLYMERASE OF THE RG-TYPE STRAIN, AND IN THE NON-CODING REGIONS OF BOTH GENOMIC SEGMENTS A TOTAL OF 4 NUCLEOTIDES THAT DO NOT COINCIDE WITH THE RG (RNA1) AND SJ (RNA2) TYPE STRAINS. ALL THESE CHANGES CAN PLAY AN IMPORTANT ROLE IN VIRULENCE AND REPLICATIVE CAPACITY. THE STUDY OF VIRULENCE DETERMINANTS (ADDITIONALLY RELATED, WITH POLYMERASE AND WITH ORF B1 AND B2) IS FUNDAMENTAL TO UNDERSTANDING AND CONTROLLING THE DISEASE, ASPECTS THAT WILL BE ADDRESSED BY THE USC RESEARCH TEAM. IT IS WELL KNOWN THAT THE DEVELOPMENT OF THE DISEASE DOES NOT DEPEND ONLY ON THE PATHOGEN, BEING DETERMINED BY VIRUS-HOST INTERACTION. THE IMPLICATION OF THE HOST IS BASED ON THE STIMULATION OF THE IMMUNE SYSTEM, WHICH WILL BE CHARACTERISED BY THE RESEARCH TEAM OF THE UMA. THE VIRIC INFECTION INDUCES THE ACTIVATION OF BOTH THE INNATE IMMUNE SYSTEM AND THE ADAPTIVE IMMUNE SYSTEM. STUDIES ON THE ACTIVATION OF THE IMMUNE SYSTEM TO BE CARRIED OUT INCLUDE PERFORMING A MASSIVE TRANSCRIPTOMIC SEQUENCING (RNA-SEQ) IN RESPONSE TO VIRUS INFECTIONS OF DIFFERENT VIRULENCE FOR SOLE. This STUDY will generate a VALIOSISIM INFORMATION ON Immunogens IMPLICED IN ANTIVIRIC RUTS OF DEFENSA, THE TIME that will allow the design of OPENARRAYS BASED IN PCR REAL TIME WITH TaqMan SOUNDS to be used to study, a LEVEL OF GENICAL EXPENDITURE, THE RESPONSE OF THE IMMUNE SYSTEM OF LINKING TO RECOMMENDANT VIRUS, OBTENTED BY GENETICA INVERSA, WITH DYSTEM LIVEL OF VIRULENCE._x000D_ Attention to this hypothesis, the PRINCIPAL OBJECTIVE OF THE PRESENT PROJECT is to analyse the FACTORS IMPLICED IN THE VIRULENCE OF Betanodavirus IN LENGUATED AND RELATION TO THE IMMUNE RESPONSE OF THE host. (English)
12 October 2021
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Málaga
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Identifiers
AGL2014-54532-C2-1-R
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