MOLECULAR REGULATION OF BIOSYNTHESIS AND TRANSPORT OF ESSENTIAL AMINO ACIDS IN MARITIM PINE (Q3140662): Difference between revisions
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MOLECULAR REGULATION OF BIOSYNTHESIS AND TRANSPORT OF ESSENTIAL AMINO ACIDS IN MARITIM PINE | |||
Revision as of 01:59, 9 October 2021
Project Q3140662 in Spain
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | MOLECULAR REGULATION OF BIOSYNTHESIS AND TRANSPORT OF ESSENTIAL AMINO ACIDS IN MARITIM PINE |
Project Q3140662 in Spain |
Statements
164,560.0 Euro
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205,700.0 Euro
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80.0 percent
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1 January 2019
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31 December 2021
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UNIVERSIDAD DE MALAGA
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29067
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EN ESTE PROYECTO SE ESTUDIARA EL METABOLISMO DEL NITROGENO DE LAS CONIFERAS UTILIZANDO ENFOQUES DE GENOMICA FUNCIONAL Y REPRESENTA LA CONTINUACION DE UN PROGRAMA DE INVESTIGACION EN BIOLOGIA MOLECULAR Y BIOTECNOLOGIA DE ARBOLES FORESTALES PREVIAMENTE FINANCIADO POR EL PROYECTO NACIONAL BIO2015-69285-R. NUESTRAS ACTIVIDADES DE INVESTIGACION SE CENTRAN EN EL PINO MARITIMO (PINUS PINASTER AIT.), UN ARBOL FORESTAL DE GRAN IMPORTANCIA ECONOMICA Y ECOLOGICA Y UNA DE LAS ESPECIES DE CONIFERAS MAS ESTUDIADAS EN INVESTIGACION GENOMICA EN EUROPA. LAS ACTIVIDADES DEL PROYECTO CAPITALIZARAN LOS AVANCES Y LOGROS RECIENTES EN EL MARCO DE LOS PROYECTOS EUROPEOS SUSTAINPINE (PROGRAMA PLANT-KBBE) Y PROCOGEN (7FP-EU) SOBRE LA GENOMICA FUNCIONAL DEL PINO MARITIMO._x000D_ PROPONEMOS ESTUDIAR LA REGULACION MOLECULAR DE LA BIOSINTESIS Y EL TRANSPORTE DE AMINOACIDOS ESENCIALES. EL PLAN DE TRABAJO INCLUYE EN LA PRIMERA TAREA ANALISIS TRANSCRIPTOMICOS Y METABOLOMICOS DE TEJIDOS DE PINO MARITIMO PARA IDENTIFICAR REDES TRANSCRIPCIONES Y RUTAS METABOLICAS REGULADAS DURANTE EL DESARROLLO Y EN RESPUESTA A LA NUTRICION CON AMINOACIDOS MARCADOS. EN LA SEGUNDA TAREA, SE SELECCIONARAN GENES ESTRUCTURALES IMPLICADOS EN LAS VIAS, LAS ENZIMAS CLAVE SE PRODUCIRAN DE FORMA RECOMBINANTE Y SE ANALIZARAN A NIVEL BIOQUIMICO PARA DETERMINAR SUS PROPIEDADES MOLECULARES Y FUNCIONALES. EN LA TERCERA TAREA, SE AISLARAN PROTEINAS DE MEMBRANA IMPLICADAS EN EL TRANSPORTE DE AMINOACIDOS ESENCIALES Y SE CARACTERIZARAN FUNCIONALMENTE. EN LA CUARTA TAREA, SE AISLARAN LOS FACTORES DE TRANSCRIPCION QUE CONTROLAN LA BIOSINTESIS DE S-ADENOSILMETIONINA, FENILALANINA Y ARGININA, Y SE INVESTIGARA LA REGULACION TRANSCRIPCIONAL DE ESTAS VIAS METABOLICAS. FINALMENTE, TAMBIEN SE PROPONEN ANALISIS FUNCIONALES DE POSIBLES REGULADORES PRINCIPALES PARA DESCIFRAR LA COMPLEJIDAD DE LA RED REGULADORA DE GENES EN EL PINO MARITIMO. LOS RESULTADOS ESPERADOS PROPORCIONARAN NUEVOS CONOCIMIENTOS Y HERRAMIENTAS MOLECULARES PARA MEJORAR LA MULTIPLICACION VEGETATIVA Y LA PRODUCCION DE BIOMASA EN EL PINO MARITIMO. (Spanish)
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THIS PROJECT WILL STUDY NITROGEN METABOLISM OF CONIFERS USING APPROACHES OF FUNCTIONAL GENOMICS AND REPRESENTS THE CONTINUATION OF A RESEARCH PROGRAMME IN MOLECULAR BIOLOGY AND BIOTECHNOLOGY OF FOREST TREES PREVIOUSLY FUNDED BY THE NATIONAL PROJECT BIO2015-69285-R. OUR RESEARCH ACTIVITIES ARE FOCUSED IN MARITIME PINE (PINUS PINASTER AIT.), A FOREST TREE OF GREAT ECONOMICAL AND ECOLOGICAL IMPORTANCE AND ONE OF THE MOST STUDIED CONIFER SPECIES FOR GENOMIC RESEARCH IN EUROPE. THE PROJECT ACTIVITIES WILL CAPITALIZE RECENT ADVANCES AND ACHIEVEMENTS IN THE FRAME OF THE EUROPEAN PROJECTS SUSTAINPINE (PLANT-KBBE PROGRAM), AND PROCOGEN (7FP-EU) ON THE FUNCTIONAL GENOMICS OF MARITIME PINE. _x000D_ WE PROPOSE TO STUDY THE MOLECULAR REGULATION OF THE BIOSYNTHESIS AND TRANSPORT OF ESSENTIAL AMINO ACIDS IN CONIFERS. THE WORKPLAN INCLUDE IN THE FIRST TASK TRANSCRIPTOMIC AND METABOLOMIC ANALYSES OF MARITIME PINE TISSUES TO IDENTIFY ESSENTIAL TRANSCRIPTS AND METABOLIC PATHWAYS REGULATED DURING DEVELOPMENT AND IN RESPONSE TO NUTRITION WITH LABELLED AMINO ACIDS. IN THE SECOND TASK, STRUCTURAL GENES INVOLVED IN THE PATHWAYS WILL BE SELECTED AND KEY ENZYMES WILL BE RECOMBINANTLY PRODUCED AND ANALYZED AT BIOCHEMICAL LEVEL TO DETERMINE THEIR MOLECULAR AND FUNCTIONAL PROPERTIES. IN THE THIRD TASK, MEMBRANE PROTEINS INVOLVED IN THE TRANSPORT OF ESSENTIAL AMINO ACIDS WILL BE ISOLATED AND FUNCTIONALLY CHARACTERIZED. IN THE FOURTH TASK, TRANSCRIPTION FACTORS CONTROLLING THE BIOSYNTHESIS OF S-ADENOSYLMETHIONINE, PHENYLALANINE AND ARGININE WILL BE ISOLATED AND THE TRANSCRIPTIONAL REGULATION OF THESE METABOLIC PATHWAYS WILL BE INVESTIGATED. FINALLY, FUNCTIONAL ANALYSES OF POTENTIAL MASTER REGULATORS ARE ALSO PROPOSED TO DECIPHERING THE COMPLEXITY OF THE GENE REGULATORY NETWORK IN MARITIME PINE. THE EXPECTED RESULTS WILL PROVIDE NEW KNOWLEDGE AND MOLECULAR TOOLS TO IMPROVE VEGETATIVE MULTIPLICATION AND BIOMASS PRODUCTION IN MARITIME PINE. (English)
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Málaga
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Identifiers
RTI2018-094041-B-I00
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